MiRNA detargeting system for tissue specific interference

The present disclosure relates to a tissue-specific promoter system for expressing microRNA (miRNA) for RNA interference-based methods of gene therapy. In these systems, the miRNA will inhibit gene expression or replace natural miRNA expression using microRNA.

1. 11. A method of inhibiting expression of the double homeobox 4 (DUX4) gene in a cell comprising contacting the cell with a recombinant adeno-associated virus comprising a nucleic acid comprising (a) a nucleotide sequence encoding a U6 promoter; (b) a nucleotide sequence encoding a mature guide strand of a miRNA targeting DUX4 mRNA, wherein the mature guide strand of the miRNA comprises the nucleotide sequence of SEQ ID NO: 7397 or 8147; (c) a nucleotide sequence comprising at least one copy of a detargeting sequence comprising the mir-122 binding site having the nucleotide sequence of SEQ ID NO: 5 or 66, and/or at least one copy of a detargeting sequence comprising the miR-208 binding site having the nucleotide sequence of SEQ ID NO: 6 or 67; and (d) 5-6 thymidines at the 5′ end.
2. 22. A method of delivering double homeobox 4 (DUX4) miRNA-encoding DNA to the skeletal muscle of an animal in need thereof, comprising contacting the skeletal muscle with a recombinant adeno-associated virus comprising a nucleic acid comprising (a) a nucleotide sequence encoding a U6 promoter; (b) a nucleotide sequence encoding a mature guide strand of a miRNA targeting DUX4 mRNA, wherein the mature guide strand of the miRNA comprises the nucleotide sequence of SEQ ID NO: 7397 or 8147; (c) a nucleotide sequence comprising at least one copy of a detargeting sequence comprising the mir-122 binding site having the nucleotide sequence of SEQ ID NO: 5 or 66, and/or at least one copy of a detargeting sequence comprising the miR-208 binding site having the nucleotide sequence of SEQ ID NO: 6 or 67; and (d) 5-6 thymidines at the 5′ end.
3. 33. A method of treating facioscapulohumeral muscular dystrophy in a subject comprising administering to the subject an effective amount of a recombinant adeno-associated virus comprising (a) a nucleotide sequence encoding a U6 promoter; (b) a nucleotide sequence encoding a mature guide strand of a miRNA targeting DUX4 mRNA, wherein the mature guide strand of the miRNA comprises the nucleotide sequence of SEQ ID NO: 7397 or 8147; (c) a nucleotide sequence comprising at least one copy of a detargeting sequence comprising the mir-122 binding site having the nucleotide sequence of SEQ ID NO: 5 or 66, and/or at least one copy of a detargeting sequence comprising the miR-208 binding site having the nucleotide sequence of SEQ ID NO: 6 or 67; and (d) 5-6 thymidines at the 5′ end.
4. 4

This application claims priority benefit of U.S. Provisional Patent Application No. 62/566,966, filed Oct. 2, 2017, which is incorporated by reference herein in its entirety.


FIELD OF THE INVENTION
The present disclosure relates to a tissue-specific promoter system for expressing microRNA (miRNA) for RNA interference-based methods of gene therapy. In these systems, the miRNA will inhibit gene expression or replace natural miRNA expression using microRNA.
INCORPORATION BY REFERENCE OF THE SEQUENCE LISTING
This application contains, as a separate part of disclosure, a Sequence Listing in computer-readable form (Filename: 52375A_SeqListing.txt; 1,684,382 bytes—ASCII text file, created Oct. 1, 2018) which is incorporated by reference herein in its entirety.
BACKGROUND
RNA interference (RNAi) is a mechanism of gene regulation in eukaryotic cells that has been considered for the treatment of various diseases. RNAi refers to post-transcriptional control of gene expression mediated by microRNAs (miRNAs). Natural miRNAs are small (21-25 nucleotides), noncoding RNAs that share sequence homology and base-pair with 3′ untranslated regions of cognate messenger RNAs (mRNAs), although regulation in coding regions may also occur. The interaction between the miRNAs and mRNAs directs cellular gene silencing machinery to degrade target mRNA and/or prevent the translation of the mRNAs. The RNAi pathway is summarized in Duan (Ed.), Section 7.3 of Chapter 7 in Muscle Gene Therapy, Springer Science+Business Media, LLC (2010).
As an understanding of natural RNAi pathways has developed, researchers have designed artificial miRNAs for use in regulating expression of target genes for treating disease. As described in Section 7.4 of Duan, supra, artificial miRNAs can be transcribed from DNA expression cassettes. The miRNA sequence specific for a target gene is transcribed along with sequences required to direct processing of the miRNA in a cell. Viral vectors such as adeno-associated virus have been used to deliver miRNAs to muscle [Fechner et al., J. Mol. Med., 86: 987-997 (2008)].
Adeno-associated virus (AAV) is a replication-deficient parvovirus, the single-stranded DNA genome of which is about 4.7 kb in length including two 145 nucleotide inverted terminal repeat (ITRs). There are multiple serotypes of AAV. The nucleotide sequences of the genomes of the AAV serotypes are known. For example, the complete genome of AAV-1 is provided in GenBank Accession No. NC_002077; the complete genome of AAV-2 is provided in GenBank Accession No. NC_001401 and Srivastava et al., J. Virol., 45: 555-564 {1983); the complete genome of AAV-3 is provided in GenBank Accession No. NC_1829; the complete genome of AAV-4 is provided in GenBank Accession No. NC_001829; the AAV-5 genome is provided in GenBank Accession No. AF085716; the complete genome of AAV-6 is provided in GenBank Accession No. NC_00 1862; at least portions of AAV-7 and AAV-8 genomes are provided in GenBank Accession Nos. AX753246 and AX753249, respectively; the AAV -9 genome is provided in Gao et al., J. Virol., 78: 6381-6388 (2004); the AAV-10 genome is provided in Mol. Ther., 13(1): 67-76 (2006); and the AAV-11 genome is provided in Virology, 330(2): 375-383 (2004). Cloning of the AAVrh.74 serotype is described in Rodino-Klapac., et al. Journal of Translational Medicine 5, 45 (2007). Isolation of the AAV-B 1 serotype is described in Choudhury et al., Mol. Therap. 24(7): 1247-57, 2016. Cis-acting sequences directing viral DNA replication (rep), encapsidation/packaging and host cell chromosome integration are contained within the AAV ITRs. Three AAV promoters (named p5, p19, and p40 for their relative map locations) drive the expression of the two AAV internal open reading frames encoding rep and cap genes. The two rep promoters (p5 and p19), coupled with the differential splicing of the single AAV intron (at nucleotides 2107 and 2227), result in the production of four rep proteins (rep 78, rep 68, rep 52, and rep 40) from the rep gene. Rep proteins possess multiple enzymatic properties that are ultimately responsible for replicating the viral genome. The cap gene is expressed from the p40 promoter and it encodes the three capsid proteins VP1, VP2, and VP3. Alternative splicing and non-consensus translational start sites are responsible for the production of the three related capsid proteins. A single consensus polyadenylation site is located at map position 95 of the AAV genome. The life cycle and genetics of AAV are reviewed in Muzyczka, Current Topics in Microbiology and Immunology, 158: 97-129 (1992).
AAV possesses unique features that make it attractive as a vector for delivering foreign DNA to cells, for example, in gene therapy. AAV infection of cells in culture is noncytopathic, and natural infection of humans and other animals is silent and asymptomatic. Moreover, AAV infects many mammalian cells allowing the possibility of targeting many different tissues in vivo. Moreover, AAV transduces slowly dividing and non-dividing cells, and can persist essentially for the lifetime of those cells as a transcriptionally active nuclear episome (extrachromosomal element). The AAV proviral genome is inserted as cloned DNA in plasmids, which makes construction of recombinant genomes feasible. Furthermore, because the signals directing AAV replication and genome encapsidation are contained within the ITRs of the AAV genome, some or all of the internal approximately 4.3 kb of the genome (encoding replication and structural capsid proteins, rep-cap) may be replaced with foreign DNA. To generate AAV vectors, the rep and cap proteins may be provided in trans. Another significant feature of AAV is that it is an extremely stable and hearty virus. It easily withstands the conditions used to inactivate adenovirus (56° to 65° C. for several hours), making cold preservation of AAV less critical. AAV may even be lyophilized. Finally, AAV-infected cells are not resistant to superinfection.
miRNA-based therapies, including miRNA inhibition and miRNA replacement, may be used to treat many diseases such as hepatitis C viral infection, muscular dystrophies, neurodegenerative diseases, peripheral neuropathies, chronic heart failure and post-myocardial infarction remodeling and cancers. In addition, miRNA directed regulation of gene expression may improve traditional gene therapy approaches in which the vector payload is a protein coding gene. Systemically delivered AAV vectors preferentially transduce the liver, resulting in high-level transgene expression in that organ if a liver-active promoter is used. As described in detail herein, the insertion of liver-specific miR-122 binding sites reduce transgene expression in the liver when a liver-specific promoter is used.
SUMMARY
The present disclosure provides for a system for tissue-specific expression of miRNA in vectors comprising detargeting miRNA binding sequences placed at various locations within the miRNA mature guide strand. The nucleic acid molecules of the disclosure comprise a tissue specific promoter and a miRNA mature guide strand comprising the corresponding binding site to detarget expression of the miRNA in a specific tissue. In particular, the disclosure provides for nucleic acid molecules comprising a tissue specific promoter sequence, mature guide strand of a miRNA comprising at least one detargeting sequence and 5-6 thymidines at the 5′ end. In one example, the nucleic acid molecules have U6 promoter and a miRNA mature guide strand containing the miR-122 and miR-208 binding site to detarget expression of the miRNA in the liver and heart, respectively. In a proof-of-concept study, liver-specific miR-122 target sequences were inserted into AAV vectors carrying luciferase or LacZ reporter genes. In these vectors, ubiquitously active U6 promoters were used to drive transcription of both genes. AAV vectors lacking miR-122 sites resulted in extremely high levels of luciferase or LacZ expression in mouse livers, while transcription of the same genes was reduced 50- and 70-fold, respectively, when delivered by vectors carrying miR-122 binding sites in each respective coding gene. Such systems have not been employed for microRNA expression vectors (Reference PMID: 21150938).
The nucleic acid molecules of the disclosure comprise any tissue specific promoter. Exemplary tissue specific promoters include U6 promoter sequence, MHCK7 promoter sequence, CK6 promoter sequence, tMCK promoter sequence, CK5 promoter sequence, MCK promoter sequence, HAS promoter sequence, MPZ promoter sequence, desmin promoter sequence, APOA2 promoter sequence, hAAT promoter sequence, INS promoter sequence, IRS2 promoter sequence, MYH6 promoter sequence, MYL2 promoter sequence, TNNI3 promoter sequence, SYN1 promoter sequence, GFAP promoter sequence, NES promoter sequence, MBP promoter sequence, or TH promoter sequence.
The disclosure provides for nucleic acid molecules comprising an U6 promoter sequence and miRNA mature guide strand sequence comprising at least one detargeting sequence and 5-6 thymidines at the 5′ end. The nucleic acid molecules of the disclosure comprise at least two detargeting sequences, at least three detargeting sequences, at least four detargeting sequences, at least five detargeting sequences or more. In addition to the tissue specific miRNA binding sites, the DNA nucleic acid sequence comprises a transcription termination signal for RNA polymerase III, which comprises five thymidines at the 5′ end or comprises six thymidines at the 5′ end. When transcribed into RNA, these thymidines are added to the transcript as uracils.
The “detargeting sequence” is the binding site for any tissue-specific miRNA that is desired to be inhibited in a tissue. For example, the disclosure provides nucleic acid sequences wherein the detargeting sequence is the binding site for any natural miRNA, for example miR-122, miR-208, miR-1, miR-206, miR-133, miR-29a, miR-29b or miR-29c. Exemplary miRNA sequences are provided in Table 2.
The nucleic acid molecules of the disclosure comprise any miRNA mature guide strand that will inhibit expression of a gene of interest. For example, the nucleic acid molecules comprise the miRNA mature guide strand of miDUX4, miRNA-92, miRNA-17, miRNA-18a, miRNA-19a, miRNA-20a, miRNA-19b-1, mi-RNA-26a, miRNA-122, miRNA-126, miRNA-335, let-7a and let-7b, miRNA-34 (miR-34a), miRNA-10b, miRNA-208, miRNA-499, miRNA-195, miRNA-29a, miRNA-29b, or miRNA-29c. The nucleic acid molecules comprise any of the miRNA mature guide strands set out as SEQ ID NOS: 10-10912. The nucleic acid molecules of the disclosure comprise the mature guide strand of miDUX4 having the nucleic acid sequence of SEQ ID NO: 1 (miDUX4-1; mi405) or SEQ ID NO: 2 (miDUX-4-2; mi1155).
The nucleic acid molecule of the disclosure comprise the mature guide stand of a miRNA comprising a nucleotide sequence of any human miRNA such as those set out in the miRBase: the microRNA database websites (miRBase.org) or Table 2. The sequences provided in the miRBase: the microRNA database are incorporated by references herein. Exemplary miRNA include but are not limited to mir450 (SEQ ID NO: 10973), mi1155mi70 (SEQ ID NO: 8482), mi180 (SEQ ID NO: 8372), mi181(SEQ ID NO: 8371), mi182 (SEQ ID NO: 8370), mi185 (SEQ ID NO: 8367), mi186 (SEQ ID NO: 8366), mi187 (SEQ ID NO: 8365), mi333 (SEQ ID NO: 8219), mi334 (SEQ ID NO: 8218), mi400 (SEQ ID NO: 8152), mi405 (SEQ ID NO: 8147), mi407 (SEQ ID NO: 8145), mi1155 (SEQ ID NO: 7397), mi1156 (SEQ ID NO: 7396), mi1157 (SEQ ID NO: 7395), mi1308 (SEQ ID NO: 7108), mi1309 (SEQ ID NO: 7107), mi1310 (SEQ ID NO: 7106), mi1420 (SEQ ID NO: 6633), mi1422 (SEQ ID NO: 6631), mi1431 (SEQ ID NO: 6622), mi1434 (SEQ ID NO: 6619), mi1444 (SEQ ID NO: 6609), mi1445 (SEQ ID NO: 6608), mi1485 (SEQ ID NO: 6568), mi1492 (SEQ ID NO: 6561), mi1493 (SEQ ID NO: 6560), mi1519 ((SEQ ID NO: 10971) or mi1520 (SEQ ID NO: 10972). These sequences fold similarly to mature guide stands of mi405 and mi1155. Therefore, the disclosure provides for nucleic acid molecules in which the mir-208 bind site sequence (SEQ ID NO: 5 or SEQ ID NO: 66) and/or the mir-122 binding site sequence (SEQ ID NO: 6 or SEQ ID NO: 67) may be inserted into the loop of any of the foregoing mature guide strand at locations similar to those set out in the sequences in Table 1.
In an exemplary embodiment, the nucleic acid molecules of the disclosure comprise a U6 promoter having the nucleic acid sequence of SEQ ID NO: 3, the mature guide strand of miDUX4 with the miR-122 and/or miR-208 binding site within the loop of the mature guide strand or at 5′ or 3′ end of the mature guide strand and 5-6 thymidines. Exemplary nucleic acid molecules comprise the miRNA mature guide strand of miDUX4 and at least one detargeting sequence, e.g. miR-122 (SEQ ID NO: 5) or miR-208 (SEQ ID NO: 6) binding sites inserted within the loop of the mature guide strand, at the 5′ end of the mature guide strand or at the 3′ end of the mature guide strand, such as the nucleic acid sequence set out as any one of SEQ ID NOS: 10913-10968.
The disclosure provides for nucleic acid molecule comprising the nucleic acid sequence of any one of SEQ ID NOS: 1, 2 or 10913-10968.
In another embodiment, the disclosure provides for recombinant adeno-associated virus (AAV) comprising any of the nucleic acid molecules of the disclosure . The AAV can be any serotype, for example AAV-B1, AAVrh.74, AAV1, AAV2, AAV3, AAV4, AAVS, AAV6, AAV7, AAV8,AAV9, AAV-10, AAV-11, AAV-12 and AAV-13. Production of pseudotyped rAAV is disclosed in, for example, WO 01/83692. Other types of rAAV variants, for example rAAV with capsid mutations, are also contemplated. See, for example, Marsic et al., Molecular Therapy, 22(11): 1900-1909 (2014). The disclosure also provides for compositions comprising any of the disclosed AAV . In addition, the disclosure provides for recombinant AAV vectors that are self-complementary AAV vectors.
In another embodiment, the disclosure provides for methods of inhibiting expression of a gene in a cell comprising contacting the cell with a vector comprising the any of the nucleic acid molecules of the. For example, the disclosure provide for methods of inhibiting expression of a gene in a cell comprising contacting the cell with a recombinant AAV comprising any of the nucleic acid molecules of the disclosure. Other embodiments of the disclosure utilize other vectors or plasmids to deliver the disclosed nucleic acid molecules, e.g. other viral vectors such as adenovirus, retrovirus, lentivirus, equine-associated virus, alphavirus, pox viruses, herpes virus, polio virus, sindbis virus and vaccinia viruses, to deliver the nucleic acid molecules of the disclosure.
The disclosure also provides for methods of inhibiting expression of the DUX4 gene in a cell comprising contacting the cell with a recombinant AAV comprising any of the disclosed nucleic acid molecules or any of the disclosed compositions. For example, the method is carried out with a nucleic acid molecule of the disclosure comprising the U6 promoter, the mature guide strand of miDUX4 with the miR-122 and/or miR-208 binding site inserted within the loop of the mature guide strand or at 5′ or 3′ end of the mature guide strand and 5-6 thymidines or the nucleic acid molecule of the disclosure comprises the nucleic acid sequence of any one of SEQ ID NOS: 10913-10968.
The disclosure provides for a use of a recombinant AAV comprising any of the disclosed nucleic acid molecules or a disclosed composition for the preparation of a medicament for inhibiting expression of the DUX4 gene in a cell. The AAV or compositions utilized to prepare the medicament comprise a nucleic acid molecule comprising the U6 promoter, the mature guide strand of miDUX4 with the miR-122 and/or miR-208 binding site inserted within the loop of the mature guide strand or at 5′ or 3′ end of the mature guide strand and 5-6 thymidines or the nucleic acid molecule comprises the nucleic acid sequence of any one of SEQ ID NOS: 10913-10968.
The disclosure also provides for a composition for the use of a recombinant AAV comprising any of the disclosed nucleic acid molecules or a disclosed composition for inhibiting expression of the DUX4 gene in a cell. The AAV or compositions utilized to prepare the medicament comprise a nucleic acid molecule comprising the U6 promoter, the mature guide strand of miDUX4 with the miR-122 and/or miR-208 binding site inserted within the loop of the mature guide strand or at 5′ or 3′ end of the mature guide strand and 5-6 thymidines or the nucleic acid molecule comprises the nucleic acid sequence of any one of SEQ ID NOS: 10913-10968.
The disclosure further provides for methods of delivering DUX4 miRNA-encoding DNA to the skeletal muscle of an animal in need thereof, comprising administering to the animal a recombinant AAV comprising the nucleic acid sequence of any one of SEQ ID NOS: 10913-10968.
The disclosure also provides for use of a recombinant AAV comprising a disclosed nucleic acid sequence for delivering DUX4 miDNA-encoding nucleic acid molecule to the skeletal muscle of an animal in need thereof. The disclosure provides for compositions comprising the nucleic acid sequence of any one of SEQ ID NOS: 10913-10968 for delivering DUX4 miDNA-encoding nucleic acid molecule to the skeletal muscle of an animal in need thereof.
In another embodiment, the disclosure provides for methods of treating facioscapulohumeral muscular dystrophy comprising administering a recombinant adeno-associated virus comprising any of the nucleic acid molecules or any of the compositions of the disclosure. For example, the method is carried out with a disclosed nucleic acid molecule comprising the U6 promoter, the mature guide strand of miDUX4 with the miR-122 and/or miR-208 binding site inserted within the loop of the mature guide strand or at 5′ or 3′ end of the mature guide strand and 5-6 thymidines or the nucleic acid molecule having the nucleic acid sequence of any one of SEQ ID NOS: 10913-10968.
In any of the methods of the disclosure, the recombinant AAV is administered by intramuscular injection, transdermal transport or injection into the blood stream
The disclosure provides for a use of a recombinant AAV comprising any disclosed nucleic acid molecule or a disclosed composition for the preparation of a medicament for treating facioscapulohumeral muscular dystrophy. The AAV or compositions utilized to prepare the medicament comprise a nucleic acid molecule of the comprising the U6 promoter, the mature guide strand of miDUX4 with the miR-122 and/or miR-208 binding site inserted within the loop of the mature guide strand or at 5′ or 3′ end of the mature guide strand and 5-6 thymidines or the nucleic acid molecule having the nucleic acid sequence of any one of SEQ ID NOS: 10913-10968.
In any of the uses of the disclosure, the medicament is formulated for administration by intramuscular injection, transdermal transport or injection into the blood stream.
The disclosure also provides for a composition for the use of a recombinant AAV comprising any disclosed nucleic acid molecules or a disclosed composition for treating facioscapulohumeral muscular dystrophy. The AAV or compositions utilized to prepare the medicament comprise a nucleic acid molecule comprising the U6 promoter, the mature guide strand of miDUX4 with the miR-122 and/or miR-208 binding site inserted within the loop of the mature guide strand or at 5′ or 3′ end of the mature guide strand and 5-6 thymidines or the nucleic acid molecule having the nucleic acid sequence of any one of SEQ ID NOS: 10913-10968 The compositions of the disclosure are formulated for administration by intramuscular injection, transdermal transport or injection into the blood stream.
DETAILED DESCRIPTION
The present disclosure provides for a system for tissue-specific specific expression of miRNA that results in tissue-specific inhibition of a gene of interest. The system comprises a promoter for tissue specific expression of a mature guide strand of a miRNA comprising detargeting sequences inserted within the mature guide strand to detarget expression of the that miRNA or gene of interest.
One example of the detargeting systems of the disclosure is the U6 promoter system for tissue-specific specific expression of miRNA. The U6 promoter system is a nucleic acid molecule comprising the U6 promoter sequence, the mature guide strand of a miRNA with detargeting sequences inserted within the mature guide strand sequence. For example, the binding site for the liver specific miR-122 and/or the binding site for the heart specific miR-208 inserted within the loop of mature guide strand of a miRNA or at the 5′ or 3′ end of the mature guide strand of a miRNA to detarget expression of the miRNA in the liver and heart, respectively.
The U6 promoter system provides, miRNA-guided inhibition of a target gene or replacement of a miRNA which may result in inhibition of a target gene or replacement of an under-transcribed miRNA. The wild type U6 promoter (U6-1) is set out as SEQ ID NO: 3.
Detargeting miRNA Sequence Expression
The promoter system of the disclosure is a nucleic acid molecule comprising a mature guide strand of a miRNA in which binding sites for detargeting miRNAs are inserted within the loop of the mature guide strand of the miRNA or at the 5′ or 3′ end of the mature guide stand of the miRNA. This system may be used with any tissue specific promoter or gene expression control element and any miRNA sequences. For example, in order to promote expression of miRNA sequence in skeletal muscle and to detarget expression of the miRNA in liver and heart tissue, the nucleic acid molecule comprises the mature guide stand of the miRNA in which the binding sites for liver specific miR-122 and/or the binding site for heart specific miR-208 are inserted within the loop of the mature guide strand or at the 5′ or 3′ end of the mature guide strand of the miRNA . The nucleotide sequence of the binding site for miRNA-122 is set out as SEQ ID NO: 5, and the nucleotide sequence of the binding site for miRNA-208 is set out as SEQ ID NO: 6.
Examples of muscle-specific promoters or muscle-specific control elements include a human skeletal actin gene element, cardiac actin gene element, myocyte-specific enhancer binding factor mef, muscle creatine kinase (MCK), truncated MCK (tMCK), myosin heavy chain (MHC), hybrid α-myosin heavy chain enhancer-/MCK enhancer-promoter (MHCK7), creatine kinase 5 (CK5) promoter, creatine kinase 6 (CK6) promoter, C5-12, murine creatine kinase enhancer element, skeletal fast-twitch troponin c gene element, desmin promoter sequence, myosin heavy chain 6 (MYH6) promoter, myosin light chain 2 (MYL2) promoter, slow-twitch cardiac troponin c gene element, the slow-twitch troponin i gene element, hypoxia-inducible nuclear factors, steroid-inducible element and glucocorticoid response element (gre).
Examples of cardiac-specific promoters include myosin heavy chain 6, cardiac muscle, alpha promoter (Myh6 or αMHC), myosin light chain 2, regulatory cardiac, slow promoter (MYL2 or MLC-2v), troponin I type 3 promoter (TNNI3 or cTnI), natriuretic peptide precursor A promoter or atrial natriuretic factor promoter (NPPA or ANF) and solute carrier family 8 (sodium/calcium exchanger) member 1 promoter (Slc8a1 or Ncx1).
Examples of liver-specific promoters include apolipoprotein A-II promoter (APOA2), Serpin peptidase inhibitor promoter, clade A (alpha-1 antiproteinase, antitrypsin), member 1 promoter, human α1 anti-trypsin promoter (hAAT), apolipoprotein A-I (APO-A1), and cytochrome P450 promoter.
Examples of pancreas-specific promoters include insulin promoter (INS), insulin receptor substrate 2 promoter (IRS2), pancreatic and duodenal homeobox 1 promoter (Pdx1), aristaless-like homoeobox 3 promoter (Alx3), and pancreatic polypeptide promoter (Ppy).
Examples of central nervous system specific promoters include synapsin I promoter (SYN1 or hSYN), glial fibrillary acidic protein promoter (GFAP), internexin neuronal intermediate filament protein, alpha promoter or α-internexin promoter (INA), nestin promoter (NES), myelin-associated oligodendrocyte basic protein promoter (MOBP), myelin basic protein promoter (MBP), myelin protein zero (MPZ) promoter, tyrosine hydroxylase promoter (TH) and forkhead box A2 promoter (FOXA2).
Examples of skin cell specific promoters include filaggrin promoter (FLG), kerarin 14 promoter (K14), and transglataminase 3 promoter (TGM3).
Examples of immune system cell specific promoters include integrin, alpha M promoter (ITGAM) and complement component 3 receptor 3 subunit promoter (CD11B)
Examples of urogenital cell specific promoters include probasin promoter (Pbsn), uroplakin 2 promoter (Upk2), spermine binding protein promoter (Sbp) and Fer-1-like 4 promoter (Fer114). An example of an endothelial cell specific promoter is endoglin promoter (ENG)
If detargeting expression of a miRNA in skeletal muscle is desired, binding sites for miR-1, miR-206 or miR-133 are inserted within the loop of the mature guide strand of the miRNA or at the 5′ or 3′ end of the mature guide strand of the miRNA.
If detargeting expression in tissues other than skeletal muscle, liver and/or heart is desired, binding sites for different miRNA transcripts may be inserted within the mature guide strand of the miRNA. For example, the miR-142 binding site may be used to detarget transcript expression in hematopoietic cells. Binding sites for miR-29a, miR-29b, and/or miR-29c may be used to detarget miRNA expression in normal tissues and to target miRNA expression in tumor tissue.
The miRNA binding sequences that may be used for detargeting miRNA expression in a tissue are collectively denoted herein as “detargeting sequences.” The nucleic acid sequence of the disclosure comprises at least one copy of a detargeting sequence, or at least two copies of a detargeting sequence, or at least three copies of a detargeting sequence, or at least four copies of the detargeting sequence or at least five copies of a detargeting sequence. The nucleic acid molecule of the disclosure comprises 1-5 copies of a detargeting sequence, or 1-4 copies of a detargeting sequence, or 1-3 copies of the detargeting sequence or 1-2 copies of the detargeting sequence, or 2-5 copies of the detargeting sequence, 2-4 copies of a detargeting sequence, or 2-3 copies of a detargeting sequence, or 3-5 copies of a detargeting sequence, or 3-4 copies of a detargeting sequence, or 4-5 copies of a detargeting sequence.
The detargeting sequences may be inserted within the loop of the mature guide strand of the miRNA or at the 5′ or 3′ end of the mature guide strand of the miRNA. Exemplary locations for insertion of the detargeting sequences are set out in FIG. 4, and exemplary nucleic acids comprising the mature guide strand of miDUX4 (mi405 (SEQ ID NO: 10973) or mi1155 (SEQ ID NO: 10974) and the miR-122 binding site (SEQ ID NO: 5 or SEQ ID NO: 66) or the miR-208 binding site (SEQ ID NO: 6 or SEQ ID NO: 67) are provided in Table 1 below.
There are two miR-208 sequences in the human and mouse genome (miR-208a and miR-208b). To avoid a run of 5 U's (pol III promoter termination sequence), in the following exemplary sequences, a single base in the binding site was mutated to a “c” (lower-case bolded “c”). This change was included because it creates a perfect binding site for mir-208b, but will have a mismatch with mir-208a.











TABLE 1








Location
Nucleotide Sequence




miR binding
of
(lower case letter -


SEQ ID

site
binding
spacers to facilitate proper


NO:
miDUX4
(underlined)
site
folding of the pre-miRNA stem)







10913
mi405
miR-122
Loop
CTCGAGTGAGCGATCCAGGATTCAGATCTGGTTTC 






 AAACCAGATCTGAATCCTGG






ACTGCCTACTAGT


 


10914
mi1155
miR-122
Loop
CTCGAGTGAGCGACAGGCGCAACCTCTCCTAGAA 






 TTCTAGGAGAGGTTGCGCCTGC






TGCCTACTAGT


 


10915
mi405
miR-122
5'end
CTCGAG TGAGC






GATCCAGGATTCAGATCTGGTTTCTGAAAGCCACAGATG






GGAAACCAGATCTGAATCCTGGACTGCCTACTAGT


 


10916
mi1155
miR-122
5'end
CTCGAG  TGAGC






GACAGGCGCAACCTCTCCTAGAACTGTAAAGCCACAGAT






GGGTTCTAGGAGAGGTTGCGCCTGCTGCCTACTAGT


 


10917
mi405
miR-122
3' end
CTCGAGTGAGCGATCCAGGATTCAGATCTGGTTTCTGAA






AGCCACAGATGGGAAACCAGATCTGAATCCTGGACTGC






CT  ACTAGT


 


10918
mi1155
miR-122
3' end
CTCGAGTGAGCGACAGGCGCAACCTCTCCTAGAACTGTA






AAGCCACAGATGGGTTCTAGGAGAGGTTGCGCCTGCTG






CCT  ACTAGT


 


10919
mi405
miR-208
Loop
CTCGAGTGAGCGATCCAGGATTCAGATCTGGTTTCT 






 GGAAACCAGATCTGAATCCT






GG ACTGCCTACTAGT


 


10920
mi1155
miR-208
Loop
CTCGAGTGAGCGACAGGCGCAACCTCTCCTAGAACT 






 GGTTCTAGGAGAGGTTGCG






CCTGCTGCCTACTAGT


 


10921
mi405
miR-208
5'end
CTCGAG  TGAGCG






ATCCAGGATTCAGATCTGGTTTCTGTAAAGCCACAGATG






GGAAACCAGATCTGAATCCTGGACTGCCTACTAGT


 


10922
mi1155
miR-208
5'end
CTCGAG  TGAGCG






ACAGGCGCAACCTCTCCTAGAACTGTAAAGCCACAGATG






GGTTCTAGGAGAGGTTGCGCCTGCTGCCTACTAGT


 


10923
mi405
miR-208
3' end
CTCGAGTGAGCGATCCAGGATTCAGATCTGGTTTCTGTA






AAGCCACAGATGGGAAACCAGATCTGAATCCTGGACTG






CCT  ACTAGT


 


10924
mi1155
miR-208
3' end
CTCGAGTGAGCGACAGGCGCAACCTCTCCTAGAACTGTA






AAGCCACAGATGGGTTCTAGGAGAGGTTGCGCCTGCTG






CCT  ACTAGT


 










10925
mi405
5' miR-122,
CTCGAG  TGAGC




3' miR-208
GATCCAGGATTCAGATCTGGTTTCTGTAAAGCCACAGAT





GGGAAACCAGATCTGAATCCTGGACTGCCT 





 ACTAGT


 


10926
mi1155
5' miR-122,
CTCGAG  GAGC




3' miR-208
GACAGGCGCAACCTCTCCTAGAACTGTAAAGCCACAGAT





GGGTTCTAGGAGAGGTTGCGCCTGCTGCCT 





 ACTAGT


 











10927
mi405
miR-122,
5' (both)
CTCGAG  




miR-208

 TGAGCGATCCAGGATTCAGATCT






GGTTTCTGTAAAGCCACAGATGGGAAACCAGATCTGAA






TCCTGGACTGCCTACTAGT


 


10928
mi1155
miR-122,
5' (both)
CTCGAG  




miR-208

 TGAGCGACAGGCGCAACCTCTC






CTAGAACTGTAAAGCCACAGATGGGTTCTAGGAGAGGT






TGCGCCTGCTGCCTACTAGT


 


10929
mi405
miR-122,
3' (both)
CTCGAGTGAGCGATCCAGGATTCAGATCTGGTTTCTGTA




miR-208

AAGCCACAGATGGGAAACCAGATCTGAATCCTGGACTG






CCT  






 ACTAGT


 


10930
mi1155
miR-122,
3' (both)
CTCGAGTGAGCGACAGGCGCAACCTCTCCTAGAACTGTA




miR-208

AAGCCACAGATGGGTTCTAGGAGAGGTTGCGCCTGCTG






CCT  






 ACTAGT


 










10931
mi405
miR-122 loop,
CTCGAG  TGAGCG




5' miR-208
ATCCAGGATTCAGATCTGGTTTCT 





 GGAAACCAGATCTGAATCCTGGACTGCCTAC





TAGT


 


10932
mi1155
miR-122 loop,
CTCGAG  TGAGCG




5' miR-208
ACAGGCGCAACCTCTCCTAGAACT 





 GGTTCTAGGAGAGGTTGCGCCTGCTGCCTAC





TAGT


 


10933
mi405
miR-122 Loop,
CTCGAGTGAGCGATCCAGGATTCAGATCTGGTTTCT 




3' miR-208
 GGAAACCAGATCTGAATCC





TGGACTGCCT  ACT





AGT


 


10934
mi1155
miR-122 Loop,
CTCGAGTGAGCGACAGGCGCAACCTCTCCTAGAACT 




3' miR-208
 GGTTCTAGGAGAGGTTGC





GCCTGCTGCCT  AC





TAGT


 


10935
mi405
miR-208 loop,
CTCGAG  T TGAGC




5' miR-122
GATCCAGGATTCAGATCTGGTTTCT 





 GGAAACCAGATCTGAATCCTGGACTGCCTACT





AGT


 


10936
mi1155
miR-208 loop,
CTCGAG  TGAGC




5' miR-122
GACAGGCGCAACCTCTCCTAGAACT 





 GGTTCTAGGAGAGGTTGCGCCTGCTGCCTAC





TAGT


 


10937
mi405
miR-208 loop,
CTCGAGTGAGCGATCCAGGATTCAGATCTGGTTTCT 




3' miR-122
 GGAAACCAGATCTGAATCCT





GGACTGCCT  AC





TAGT


 


10938
mi1155
miR-208 loop,
CTCGAGTGAGCGACAGGCGCAACCTCTCCTAGAACT 




3' miR-122
 GGTTCTAGGAGAGGTTGCG





CCTGCTGCCT TATTTAGTGTGATAATGGTGTTTA





CTAGT


 











10939
mi405
miR-122
Loop
CTCGAGTGAGCGATCCAGGATTCAGATCTGGTTTCT 




miR-122
(both)
  






 GGAAACCAGATCTGAATCCTGGACTGCCTACTAGT


 


10940
mi1155
miR-122
Loop
CTCGAGTGAGCGACAGGCGCAACCTCTCCTAGAACT 




miR-122
(both)
  






 GGTTCTAGGAGAGGTTGCGCCTGCTGCCTACTAGT


 


10941
mi405
miR-122
Loop
CUCGAGUGAGCGAUCCAGGAUUCAGAUCUGGUUUC 






  AAACCAGAUCUGA






AUCCUGGACUGCCUACUAGU


 


10942
mi1155
miR-122
Loop
CUCGAGUGAGCGACAGGCGCAACCUCUCCUAGAA 






 UUCUAGGAGAGGUUGCG






CCUGCUGCCUACUAGU


 


10943
mi405
miR-122
5'end
CUCGAG  U






GAGCGAUCCAGGAUUCAGAUCUGGUUUCUGAAAGCC






ACAGAUGGGAAACCAGAUCUGAAUCCUGGACUGCCU






ACUAGU


 


10944
mi1155
miR-122
5'end
CUCGAG  U






GAGCGACAGGCGCAACCUCUCCUAGAACUGUAAAGCC






ACAGAUGGGUUCUAGGAGAGGU UGCGCCUGCUGCCU






ACUAGU


 


10945
mi405
miR-122
3' end
CUCGAGUGAGCGAUCCAGGAUUCAGAUCUGGUUUCU






GAAAGCCACAGAUGGGAAACCAGAUCUGAAUCCUGGA






CUGCCU  A






CUAGU


 


10946
mi1155
miR-122
3' end
CUCGAGUGAGCGACAGGCGCAACCUCUCCUAGAACUG






UAAAGCCACAGAUGGGUUCUAGGAGAGGUUGCGCCU






GCUGCCU  






ACUAGU


 


10947
mi405
miR-208
Loop
CUCGAGUGAGCGAUCCAGGAUUCAGAUCUGGUUUCU






  GGAAACCAGAUCUG






AAUCCUGGACUGCCUACUAGU


 


10948
mi1155
miR-208
Loop
CUCGAGUGAGCGACAGGCGCAACCUCUCCUAGAACU 






 GGUUCUAGGAGAGG






UUGCGCCUGCUGCCUACUAGU


 


10949
mi405
m i R-208
5'end
CUCGAG  UGA






GCGAUCCAGGAUUCAGAUCUGGUUUCUGUAAAGCCA






CAGAUGGGAAACCAGAUCUGAAUCCUGGACUGCCUAC






UAGU


 


10950
mi1155
miR-208
5'end
CUCGAG  UGA






GCGACAGGCGCAACCUCUCCUAGAACUGUAAAGCCAC






AGAUGGGUUCUAGGAGAGGUUGCGCCUGCUGCCUAC






UAGU


 


10951
mi405
miR-208
3' end
CUCGAGUGAGCGAUCCAGGAUUCAGAUCUGGUUUCU






GUAAAGCCACAGAUGGGAAACCAGAUCUGAAUCCUG






GACUGCCU  A






CUAGU


 


10952
mi1155
miR-208
3' end
CUCGAGUGAGCGACAGGCGCAACCUCUCCUAGAACUG






UAAAGCCACAGAUGGGUUCUAGGAGAGGUUGCGCCU






GCUGCCU  AC






UAGU


 










10953
mi405
5' miR-122,
CUCGAG  U




3' miR-208
GAGCGAUCCAGGAUUCAGAUCUGGUUUCUGUAAAGC





CACAGAUGGGAAACCAGAUCUGAAUCCUGGACUGCCU





  ACUAGU


 


10954
mi1155
5' miR-122,
CUCGAG  U




3' miR-208
GAGCGACAGGCGCAACCUCUCCUAGAACUGUAAAGCC





ACAGAUGGGUUCUAGGAGAGGUUGCGCCUGCUGCCU





  ACUAGU


 











10955
mi405
miR-122,
5' (both)
CUCGAG  




miR-208

 UGAGCGAUCCAGGAU






UCAGAUCUGGUUUCUGUAAAGCCACAGAUGGGAAAC






CAGAUCUGAAUCCUGGACUGCCUACUAGU


 


10956
mi1155
miR-122,
5' (both)
CUCGAG  




miR-208

  UGAGCGACAGGCGCA






ACCUCUCCUAGAACUGUAAAGCCACAGAUGGGUUCUA






GGAGAGGUUGCGCCUGCUGCCUACUAGU


 


10957
mi405
miR-122,
3' (both)
CUCGAGUGAGCGAUCCAGGAUUCAGAUCUGGUUUCU




miR-208

GUAAAGCCACAGAUGGGAAACCAGAUCUGAAUCCUG






GACUGCCU  






  ACUAGU


 


10958
mi1155
miR-122,
3' (both)
CUCGAGUGAGCGACAGGCGCAACCUCUCCUAGAACUG




miR-208

UAAAGCCACAGAUGGGUUCUAGGAGAGGUUGCGCCU






GCUGCCU  






  ACUAGU


 










10959
mi405
miR-122 Loop,
CUCGAG  UGA




5' miR-208
GCGAUCCAGGAUUCAGAUCUGGUUUCU 





 GGAAACCAGAUCUGAAUCCU





GGACUGCCUACUAGU


 


10960
mi1155
miR-122 Loop,
CUCGAG  UGA




5' miR-208
GCGACAGGCGCAACCUCUCCUAGAACU 





 GGUUCUAGGAGAGGUUGCGCC





UGCUGCCUACUAGU


 


10961
mi405
miR-122 Loop,
CUCGAGUGAGCGAUCCAGGAUUCAGAUCUGGUUUCU




3' miR-208
  GGAAACCAGAUC





UGAAUCCUGGACUGCCU 





 ACUAGU


 


10962
mi1155
miR-122 Loop,
CUCGAGUGAGCGACAGGCGCAACCUCUCCUAGAACU 




3' miR-208
  GGUUCUAGGAGA





GGUUGCGCCUGCUGCCU 





 ACUAGU


 


10963
mi405
miR-208 Loop,
CUCGAG  U




5' miR-122
GAGCGAUCCAGGAUUCAGAUCUGGUUUCU 





 GGAAACCAGAUCUGAAUCCUG





GACUGCCUACUAGU


 


10964
mi1155
miR-208 loop,
CUCGAG  U




5' miR-122
GAGCGACAGGCGCAACCUCUCCUAGAACU 





 GGUUCUAGGAGAGGUUGCGCC





UGCUGCCUACUAGU


 


10965
mi405
miR-208 loop,
CUCGAGUGAGCGAUCCAGGAUUCAGAUCUGGUUUCU




3' miR-122
  GGAAACCAGAUCUG





AAUCCUGGACUGCCU  





 ACUAGU


 


10966
mi1155
miR-208 loop,
CUCGAGUGAGCGACAGGCGCAACCUCUCCUAGAACU 




3' miR-122
  GGUUCUAGGAGAGG





UUGCGCCUGCUGCCU  





 ACUAGU


 











10967
mi405
miR-122
Loop
CUCGAGUGAGCGAUCCAGGAUUCAGAUCUGGUUUCU




miR-122
(both)
  






 GGAAACCAGAUCUGAAUCCUGGACUG






CCUACUAGU


 


10968
mi1155
miR-122
Loop
CUCGAGUGAGCGACAGGCGCAACCUCUCCUAGAACU 




miR-122
(both)
  






 GGUUCUAGGAGAGGUUGCGCCUGCUG






CCUACUAGU









The mature guide stand of a miRNA comprising a nucleotide sequence of mi70 (SEQ ID NO: 8482), mi180 (SEQ ID NO: 8372), mi181(SEQ ID NO: 8371), mi182 (SEQ ID NO: 8370), mi185 (SEQ ID NO: 8367), mi186 (SEQ ID NO: 8366), mi187 (SEQ ID NO: 8365), mi333 (SEQ ID NO: 8219), mi334 (SEQ ID NO: 8218), mi400 (SEQ ID NO: 8152), mi405 (SEQ ID NO: 8147), mi407 (SEQ ID NO: 8145), mi1155 (SEQ ID NO: 7397), mi1156 (SEQ ID NO: 7396), mi1157 (SEQ ID NO: 7395), mi1308 (SEQ ID NO: 7108), mi1309 (SEQ ID NO: 7107), mi1310 (SEQ ID NO: 7106), mi1420 (SEQ ID NO: 6633), mi1422 (SEQ ID NO: 6631), mi1431 (SEQ ID NO: 6622), mi1434 (SEQ ID NO: 6619), mi1444 (SEQ ID NO: 6609), mi1445 (SEQ ID NO: 6608), mi1485 (SEQ ID NO: 6568), mi1492 (SEQ ID NO: 6561), mi1493 (SEQ ID NO: 6560), mi1519 ((SEQ ID NO: 10971) or mi1520 (SEQ ID NO: 10972) may be used. These sequences fold similarly to mature guide stands of mi405 and mi1155 fold similarly to the mature guide strands of mir405 and mir1155. Therefore, the disclosure provides for nucleic acid molecules in which the mir-208 bind site sequence (SEQ ID NO: 5 or SEQ ID NO: 66) and/or the mir-122 binding site sequence (SEQ ID NO: 6 or SEQ ID NO: 67) may be inserted into the loop of any of the foregoing mature guide strands at locations similar to those set out in the sequences in Table 1.
miRNA of Interest
The nucleic acid molecules of the disclosure may comprise the sequence of the mature guide strand of any miRNA transcript sequence desired to have tissue-specific expression. For example, in one embodiment, skeletal expression of DUX4 miRNA is contemplated. Exemplary DUX4 miRNA sequences are provided in International Patent Application No. PCT/US2012/047999 (WO 2013/016352) and US patent publication no. US 201220225034 incorporated by reference herein in their entirety.
Two examples of miDUX4 are miDUX4-1 (miDux405; SEQ ID NO: 1): and miDUX4-2 (miDux1155; SEQ ID NO: 2). Exemplary nucleotide sequences comprising the DUX4 miRNA and the binding site for either miR-122 or miR-208 are provided in Table 1 and SEQ ID NOS: 10913-10968.
Any human miRNA may be expressed using the nucleic acid molecules of the disclosure , including those set out in the miRBase: the microRNA database websites (miRBase.org), which are incorporated by reference herein in its entirety. Examples include but not limited to the following: miR-122, miR-124, miR-142, miR-155, miR-21, miR-17-92, miR-17, miR-18a, miR-19a, miR-20a, miR-19b-1, miR-26a, miR-126, miR-335, let-7 family: let-7a and let-7b, miR-34 (miR-34a), miR-10b, miR-208, miR-499, miR-195, miR-29a, miR-29b, and miR-29c. Additional exemplary miRNA are set out below in Table 2. Any of these miRNA may be used with different detargeting sequences, depending of the desired tissue specificity and desired detargeting.














TABLE 2





miRNA ID
Accession
RPM
Chromosome
Start
End
Strand
Confidence






















hsa-mir-99b
MI0000746
1742
chr19
51692612
51692681
+
✓


 


hsa-mir-99a
MI0000101
26393
chr21
16539089
16539169
+
✓


 


hsa-mir-9986
MI0032316
—
chr2
132433711
132433820
+
—


 


hsa-mir-9985
MI0032313
1873
chrY
4606120
4606228
+
✓


 


hsa-mir-9983
MI0033670
3
chr6
39074314
39074381
+
—


 


hsa-mir-9903
MI0031831
1
chr8
98393645
98393738
−
✓


 


hsa-mir-9902-2
MI0041071
406
chr12
122709332
122709424
−
—


 


hsa-mir-9902-1
MI0031830
406
chr12
122695907
122695999
−
—


 


hsa-mir-9901
MI0031829
13
chr16
34163578
34163673
+
—


 


hsa-mir-9900
MI0031828
—
chr3
112737089
112737181
+
—


 


hsa-mir-9899
MI0031827
—
chr2
149330278
149330373
−
—


 


hsa-mir-9898
MI0031826
3
chr12
52898889
52898984
−
—


 


hsa-mir-9851
MI0039502
—
chr10
116827488
116827568
−
—


 


hsa-mir-98
MI0000100
45139
chrX
53556223
53556341
−
✓


 


hsa-mir-9718
MI0030983
—
chr14
60648648
60648711
+
—


 


hsa-mir-96
MI0000098
483
chr7
129774692
129774769
−
✓


 


hsa-mir-9500
MI0029185
—
chr2
218823090
218823154
+
—


 


hsa-mir-95
MI0000097
164
chr4
8005301
8005381
−
—


 


hsa-mir-944
MI0005769
80
chr3
189829922
189830009
+
—


 


hsa-mir-943
MI0005768
—
chr4
1986384
1986477
−
—


 


sa-mir-942
MI0005767
2
chr1
117094643
117094728
+
✓


 


hsa-mir-941-5
MI0031520
57
chr20
63919868
63919939
+
✓


 


hsa-mir-941-4
MI0005766
57
chr20
63919756
63919827
+
✓


 


hsa-mir-941-3
MI0005765
57
chr20
63919561
63919632
+
✓


 


hsa-mir-941-2
MI0005764
57
chr20
63919505
63919576
+
✓


 


hsa-mir-941-1
MI0005763
57
chr20
63919449
63919520
+
✓


 


hsa-mir-940
MI0005762
—
chr16
2271747
2271840
+
—


 


hsa-mir-939
MI0005761
—
chr8
144394149
144394230
−
—


 


hsa-mir-938
MI0005760
53
chr10
29602264
29602346
−
—


 


hsa-mir-937
MI0005759
18
chr8
143812957
143813042
−
—


 


hsa-mir-936
MI0005758
—
chr10
104048089
104048186
−
—


 


hsa-mir-935
MI0005757
11
chr19
53982307
53982397
+
—


 


hsa-mir-934
MI0005756
5
chrX
136550878
136550960
+
—


 


hsa-mir-933
MI0005755
—
chr2
175167633
175167709
−
—


 


hsa-mir-93
MI0000095
2001
chr7
100093768
100093847
−
✓


 


hsa-mir-92b
MI0003560
4600
chr1
155195177
155195272
+
✓


 


hsa-mir-92a-2
MI0000094
7185
chrX
134169538
134169612
−
✓


 


hsa-mir-92a-1
MI0000093
8600
chr13
91351314
91351391
+
✓


 


hsa-mir-924
MI0005716
100
chr18
39622123
39622175
−
—


 


hsa-mir-922
MI0005714
17
chr3
197674496
197674576
−
—


 


hsa-mir-921
MI0005713
—
chr1
166154743
166154798
−
—


 


hsa-mir-920
MI0005712
—
chr12
24212421
24212495
+
—


 


hsa-mir-9-3
MI0000468
1073
chr15
89368017
89368106
+
✓


 


hsa-mir-9-2
MI0000467
1073
chr5
88666853
88666939
−
✓


 


hsa-mir-9-1
MI0000466
1073
chr1
156420341
156420429
−
✓


 


hsa-mir-892c
MI0022560
2
chrX
145992750
145992826
−
—


 


hsa-mir-892b
MI0005538
—
chrX
145997198
145997274
−
—


 


hsa-mir-892a
MI0005528
—
chrX
145996669
145996743
−
—


 


hsa-mir-891b
MI0005534
—
chrX
146001053
146001131
−
—


 


hsa-mir-891a
MI0005524
—
chrX
146027794
146027872
−
—


 


hsa-mir-890
MI0005533
2
chrX
145994275
145994351
−
—


 


hsa-mir-889
MI0005540
26
chr14
101047901
101047979
+
✓


 


hsa-mir-888
MI0005537
784
chrX
145994784
145994860
−
✓


 


hsa-mir-887
MI0005562
6
chr5
15935182
15935260
+
✓


 


hsa-mir-885
MI0005560
4
chr3
10394489
10394562
−
✓


 


hsa-mir-877
MI0005561
70
chr6
30584332
30584417
+
—


 


hsa-mir-876
MI0005542
28
chr9
28863626
28863706
−
✓


 


hsa-mir-875
MI0005541
—
chr8
99536786
99536861
−
—


 


hsa-mir-874
MI0005532
23
chr5
137647572
137647649
−
✓


 


hsa-mir-873
MI0005564
8
chr9
28888879
28888955
−
✓


 


hsa-mir-8485
MI0027288
1000
chr2
50696172
50696262
−
—


 


hsa-mir-8089
MI0025925
28
chr5
181043403
181043484
−
—


 


hsa-mir-8088
MI0025924
—
chrX
52336557
52336642
−
—


 


hsa-mir-8087
MI0025923
65
chr11
27514970
27515047
−
—


 


hsa-mir-8086
MI0025922
209
chr10
28289258
28289350
−
—


 


hsa-mir-8085
MI0025921
—
chr19
44758657
44758721
+
—


 


hsa-mir-8084
MI0025920
—
chr8
93029751
93029839
+
—


 


hsa-mir-8083
MI0025919
—
chr1
153689705
153689793
−
—


 


hsa-mir-8082
MI0025918
—
chr4
113152282
113152362
+
—


 


hsa-mir-8081
MI0025917
472
chr9
106600928
106601022
+
—


 


hsa-mir-8080
MI0025916
15
chr2
79866495
79866583
−
—


 


hsa-mir-8079
MI0025915
—
chr13
44196129
44196200
−
—


 


hsa-mir-8078
MI0025914
—
chr18
112256
112339
−
—


 


hsa-mir-8077
MI0025913
—
chr19
42351131
42351205
+
—


 


hsa-mir-8076
MI0025912
—
chr3
113432118
113432200
−
—


 


hsa-mir-8075
MI0025911
—
chr13
113262920
113262999
+
—


 


hsa-mir-8074
MI0025910
—
chr19
51206929
51207009
−
—


 


hsa-mir-8073
MI0025909
—
chr13
110340958
110341029
+
—


 


hsa-mir-8072
MI0025908
5
chr12
123364764
123364843
−
—


 


hsa-mir-8071-2
MI0026417
—
chr14
105640168
105640232
+
—


 


hsa-mir-8071-1
MI0025907
—
chr14
105621116
105621180
+
—


 


hsa-mir-8070
MI0025906
—
chr11
11783135
11783222
−
—


 


hsa-mir-8069-2
MI0031519
12
chr21
13724189
13724274
+
—


 


hsa-mir-8069-1
MI0025905
12
chr21
6859171
6859256
+
—


 


hsa-mir-8068
MI0025904
—
chr11
28477481
28477548
−
—


 


hsa-mir-8067
MI0025903
—
chr15
62304658
62304734
−
—


 


hsa-mir-8066
MI0025902
—
chr4
101240795
101240872
−
—


 


hsa-mir-8065
MI0025901
180
chr16
5632467
5632566
+
—


 


hsa-mir-8064
MI0025900
3
chr3
52846463
52846552
−
—


 


hsa-mir-8063
MI0025899
—
chr15
36972821
36972901
−
—


 


hsa-mir-8062
MI0025898
—
chr20
7371608
7371692
+
—


 


hsa-mir-8061
MI0025897
—
chr19
54645309
54645383
+
—


 


hsa-mir-8060
MI0025896
—
chr3
96359964
96360039
+
—


 


hsa-mir-8059
MI0025895
—
chr17
50768650
50768730
+
—


 


hsa-mir-8058
MI0025894
8
chr16
82688931
82689019
+
—


 


hsa-mir-8057
MI0025893
5
chr18
26591467
26591535
−
—


 


hsa-mir-8056
MI0025892
—
chr5
173347455
173347536
+
—


 


hsa-mir-8055
MI0025891
—
chr8
6622124
6622220
−
—


 


hsa-mir-8054
MI0025890
—
chr11
23419105
23419190
−
—


 


hsa-mir-8053
MI0025889
—
chr4
47652669
47652743
+
—


 


hsa-mir-8052
MI0025888
—
chr11
130666735
130666803
+
—


 


hsa-mir-802
MI0003906
266
chr21
35720715
35720808
+
—


 


hsa-mir-7978
MI0025754
—
chr4
21464700
21464758
−
—


 


hsa-mir-7977
MI0025753
58
chr3
176515103
176515151
+
—


 


hsa-mir-7976
MI0025752
—
chr3
127587111
127587176
−
✓


 


hsa-mir-7975
MI0025751
121
chr19
55123225
55123292
−
—


 


hsa-mir-7974
MI0025750
21
chr19
11495544
11495622
−
—


 


hsa-mir-7973-2
MI0025749
—
chr15
51314032
51314107
−
—


 


hsa-mir-7973-1
MI0025748
—
chr15
51314034
51314109
+
—


 


hsa-mir-7856
MI0025526
—
chr1
86357632
86357687
−
—


 


hsa-mir-7855
MI0025525
35
chr14
64785626
64785686
−
—


 


hsa-mir-7854
MI0025524
—
chr16
81533902
81533966
+
—


 


hsa-mir-7853
MI0025523
11
chr6
6169304
6169435
−
—


 


hsa-mir-7852
MI0025522
—
chr1
107897223
107897304
+
—


 


hsa-mir-7851
MI0025521
837
chr12
42323700
42323859
−
—


 


hsa-mir-7850
MI0025520
64
chr19
2630715
2630793
+
—


 


hsa-mir-7849
MI0025519
—
chr4
146408583
146408688
+
—


 


hsa-mir-7848
MI0025518
—
chr8
133046481
133046581
−
—


 


hsa-mir-7847
MI0025517
24
chr11
1880045
1880147
+
—


 


hsa-mir-7846
MI0025516
4
chr1
12166943
12167038
+
—


 


hsa-mir-7845
MI0025515
4
chr2
207166400
207166498
+
—


 


hsa-mir-7844
MI0025514
—
chr12
94571231
94571352
−
—


 


hsa-mir-7843
MI0025510
—
chr14
72516820
72516898
+
—


 


hsa-mir-7706
MI0025242
1
chr15
85380596
85380662
+
—


 


hsa-mir-7705
MI0025241
—
chr8
100702968
100703024
−
—


 


hsa-mir-7704
MI0025240
29
chr2
176188843
176188901
+
—


 


hsa-mir-7703
MI0025239
4
chr14
24143489
24143565
−
—


 


hsa-mir-7702
MI0025238
—
chr9
111271156
111271214
−
—


 


hsa-mir-770
MI0005118
—
chr14
100852390
100852487
+
✓


 


hsa-mir-769
MI0003834
120
chr19
46018932
46019049
+
✓


 


hsa-mir-767
MI0003763
30
chrX
152393421
152393529
−
✓


 


hsa-mir-766
MI0003836
10
chrX
119646738
119646848
−
✓


 


hsa-mir-765
MI0005116
8
chr1
156936131
156936244
−
—


 


hsa-mir-764
MI0003944
—
chrX
114639435
114639519
+
—


 


hsa-mir-762
MI0003892
—
chr16
30893903
30893985
+
—


 


hsa-mir-761
MI0003941
40
chr1
51836344
51836402
−
—


 


hsa-mir-760
MI0005567
9
chr1
93846832
93846911
+
—


 


hsa-mir-759
MI0004065
—
chr13
52810050
52810140
+
—


 


hsa-mir-758
MI0003757
2
chr14
101026020
101026107
+
✓


 


hsa-mir-7515
MI0024354
20
chr2
6650373
6650439
+
—


 


hsa-mir-744
MI0005559
324
chr17
12081899
12081996
+
✓


 


hsa-mir-718
MI0012489
2
chrX
154019920
154019989
−
—


 


hsa-mir-7162
MI0023623
—
chr10
30368597
30368665
−
—


 


hsa-mir-7161
MI0023619
107
chr6
158609707
158609790
+
—


 


hsa-mir-7160
MI0023621
—
chr8
2076589
2076640
+
—


 


hsa-mir-7159
MI0023620
50
chr6
33899135
33899200
+
—


 


hsa-mir-7158
MI0023618
—
chr2
5974662
5974732
+
—


 


hsa-mir-7157
MI0023617
—
chr2
140586626
140586685
−
—


 


hsa-mir-7156
MI0023616
—
chr1
77060143
77060202
+
—


 


hsa-mir-7155
MI0023615
—
chr11
64341849
64341904
−
—


 


hsa-mir-7154
MI0023614
—
chr11
45691704
45691776
−
✓


 


hsa-mir-7153
MI0023613
—
chr18
11654885
11654941
−
—


 


hsa-mir-7152
MI0023612
—
chr10
71790747
71790800
+
—


 


hsa-mir-7151
MI0023611
—
chr10
67403351
67403410
−
—


 


hsa-mir-7150
MI0023610
—
chr9
123485529
123485622
−
—


 


hsa-mir-7114
MI0022965
—
chr9
137450026
137450086
−
—


 


hsa-mir-7113
MI0022964
—
chr11
68032864
68032922
+
—


 


hsa-mir-7112
MI0022963
—
chr8
144262673
144262737
−
—


 


hsa-mir-7111
MI0022962
—
chr6
35470508
35470579
+
—


 


hsa-mir-7110
MI0022961
578
chr3
123161794
123161879
+
—


 


hsa-mir-711
MI0012488
—
chr3
48578902
48578977
−
—


 


hsa-mir-7109
MI0022960
2
chr22
31621467
31621531
−
—


 


hsa-mir-7108
MI0022959
100
chr19
2434914
2435000
−
—


 


hsa-mir-7107
MI0022958
13
chr12
121444273
121444352
−
—


 


hsa-mir-7106
MI0022957
—
chr12
113159113
113159177
−
—


 


hsa-mir-708
MI0005543
184
chr11
79402022
79402109
−
✓


 


hsa-mir-7-3
MI0000265
3279
chr19
4770670
4770779
+
—


 


hsa-mir-7-2
MI0000264
3265
chr15
88611825
88611934
+
✓


 


hsa-mir-7-1
MI0000263
3664
chr9
83969748
83969857
−
✓


 


hsa-mir-6895
MI0022742
4
chrX
53195411
53195488
−
—


 


hsa-mir-6894
MI0022741
1
chrX
53198889
53198945
−
—


 


hsa-mir-6893
MI0022740
—
chr8
144435551
144435619
−
—


 


hsa-mir-6892
MI0022739
35
chr7
143382686
143382800
+
—


 


hsa-mir-6891
MI0022738
12
chr6
31355224
31355316
−
—


 


hsa-mir-6890
MI0022737
—
chr3
49099854
49099914
−
—


 


hsa-mir-6889
MI0022736
—
chr22
41252992
41253050
−
—


 


hsa-mir-6888
MI0022735
2
chr2
159186835
159186901
+
—


 


hsa-mir-6887
MI0022734
—
chr19
35122700
35122764
+
—


 


hsa-mir-6886
MI0022733
—
chr19
11113474
11113534
+
—


 


hsa-mir-6885
MI0022732
—
chr19
6389638
6389703
−
—


 


hsa-mir-6884
MI0022731
—
chr17
40026332
40026409
−
—


 


hsa-mir-6883
MI0022730
—
chr17
8144994
8145071
−
—


 


hsa-mir-6882
MI0022729
29
chr15
74840642
74840707
−
—


 


hsa-mir-6881
MI0022728
—
chr15
74411357
74411432
−
—


 


hsa-mir-6880
MI0022727
2
chr12
124337181
124337242
−
—


 


hsa-mir-6879
MI0022726
162
chr11
65018505
65018570
+
—


 


hsa-mir-6878
MI0022725
69
chr1
150492345
150492410
+
—


 


hsa-mir-6877
MI0022724
—
chr9
133051996
133052059
+
—


 


hsa-mir-6876
MI0022723
12
chr8
25345402
25345474
+
—


 


hsa-mir-6875
MI0022722
49
chr7
100868036
100868107
+
—


 


hsa-mir-6874
MI0022721
18
chr7
5711840
5711910
−
—


 


hsa-mir-6873
MI0022720
72
chr6
33287227
33287289
−
—


 


hsa-mir-6872
MI0022719
—
chr3
50273236
50273297
+
—


 


hsa-mir-6871
MI0022718
—
chr20
41169023
41169078
+
—


 


hsa-mir-6870
MI0022717
2
chr20
10649636
10649695
−
—


 


hsa-mir-6869
MI0022716
—
chr20
1392900
1392961
−
—


 


hsa-mir-6868
MI0022715
—
chr17
76098019
76098076
−
—


 


hsa-mir-6867
MI0022714
4
chr17
40193597
40193663
+
—


 


hsa-mir-6866
MI0022713
—
chr17
40161933
40162001
+
—


 


hsa-mir-6865
MI0022712
—
chr17
4970086
4970150
−
—


 


hsa-mir-6864
MI0022711
—
chr17
4969702
4969771
−
—


 


hsa-mir-6863
MI0022710
—
chr16
56904264
56904353
+
—


 


hsa-mir-6862-2
MI0026415
—
chr16
28724252
28724321
+
—


 


hsa-mir-6862-1
MI0022709
—
chr16
28390982
28391051
−
—


 


hsa-mir-6861
MI0022708
6
chr12
112163258
112163321
−
—


 


hsa-mir-6860
MI0022707
—
chr11
67045643
67045708
+
—


 


hsa-mir-6859-4
MI0031521
—
chr16
17052
17119
−
—


 


hsa-mir-6859-3
MI0026421
—
chr15
101973524
101973591
+
—


 


hsa-mir-6859-2
MI0026420
—
chr1
187891
187958
−
—


 


hsa-mir-6859-1
MI0022705
—
chr1
17369
17436
−
—


 


hsa-mir-6858
MI0022704
19
chrX
154450320
154450386
+
—


 


hsa-mir-6857
MI0022703
—
chrX
53405673
53405765
−
—


 


hsa-mir-6856
MI0022702
47
chr9
130626297
130626363
+
—


 


hsa-mir-6855
MI0022701
—
chr9
129869605
129869671
+
✓


 


hsa-mir-6854
MI0022700
—
chr9
98229149
98229217
−
—


 


hsa-mir-6853
MI0022699
—
chr9
35732922
35732995
+
—


 


hsa-mir-6852
MI0022698
—
chr9
35710676
35710741
−
—


 


hsa-mir-6851
MI0022697
2
chr9
33467869
33467935
−
—


 


hsa-mir-6850
MI0022696
—
chr8
144791931
144791991
−
—


 


hsa-mir-6849
MI0022695
—
chr8
144400277
144400345
−
—


 


hsa-mir-6848
MI0022694
—
chr8
144317246
144317315
−
—


 


hsa-mir-6847
MI0022693
—
chr8
144079874
144079942
+
—


 


hsa-mir-6846
MI0022692
20
chr8
144057321
144057380
−
—


 


hsa-mir-6845
MI0022691
—
chr8
143837756
143837816
−
—


 


hsa-mir-6844
MI0022690
—
chr8
124508515
124508576
−
—


 


hsa-mir-6843
MI0022689
—
chr8
27610601
27610751
−
—


 


hsa-mir-6842
MI0022688
34
chr8
27433370
27433434
+
—


 


hsa-mir-6841
MI0022687
—
chr8
24953796
24953867
−
—


 


hsa-mir-6840
MI0022686
—
chr7
100356651
100356721
+
—


 


hsa-mir-6839
MI0022685
—
chr7
64679064
64679176
+
—


 


hsa-mir-6838
MI0022684
—
chr7
44073378
44073433
−
—


 


hsa-mir-6837
MI0022683
—
chr7
44051766
44051829
+
—


 


hsa-mir-6836
MI0022682
10
chr7
2257515
2257577
−
—


 


hsa-mir-6835
MI0022680
—
chr6
34240673
34240736
+
—


 


hsa-mir-6834
MI0022679
—
chr6
33290245
33290325
+
—


 


hsa-mir-6833
MI0022678
1
chr6
32179816
32179876
+
—


 


hsa-mir-6832
MI0022677
12
chr6
31633787
31633858
+
—


 


hsa-mir-6831
MI0022676
—
chr5
140563671
140563751
−
—


 


hsa-mir-6830
MI0022675
—
chr5
132217849
132217918
−
—


 


hsa-mir-6829
MI0022674
—
chr3
195882329
195882395
−
—


 


hsa-mir-6828
MI0022673
—
chr3
170423103
170423162
+
—


 


hsa-mir-6827
MI0022672
—
chr3
134367804
134367862
−
—


 


hsa-mir-6826
MI0022671
3
chr3
129272146
129272243
+
—


 


hsa-mir-6825
MI0022670
—
chr3
127575266
127575331
−
—


 


hsa-mir-6824
MI0022669
—
chr3
48633636
48633698
−
—


 


hsa-mir-6823
MI0022668
—
chr3
48549961
48550021
−
—


 


hsa-mir-6822
MI0022667
3
chr3
39138206
39138266
+
—


 


hsa-mir-6821
MI0022666
5
chr22
49962866
49962939
+
—


 


hsa-mir-6820
MI0022665
—
chr22
37967563
37967624
+
✓


 


hsa-mir-6819
MI0022664
—
chr22
36286847
36286907
−
—


 


hsa-mir-6818
MI0022663
5
chr22
30007049
30007113
+
—


 


hsa-mir-6817
MI0022662
—
chr22
25455646
25455711
+
—


 


hsa-mir-6816
MI0022661
—
chr22
20114686
20114751
−
—


 


hsa-mir-6815
MI0022660
—
chr21
45478266
45478326
+
—


 


hsa-mir-6814
MI0022659
—
chr21
41746772
41746841
−
—


 


hsa-mir-6813
MI0022658
—
chr20
64076955
64077010
−
—


 


hsa-mir-6812
MI0022657
90
chr20
45425510
45425573
+
—


 


hsa-mir-6811
MI0022656
—
chr2
237510931
237510988
+
—


 


hsa-mir-6810
MI0022655
—
chr2
218341911
218341980
+
—


 


hsa-mir-6809
MI0022654
2
chr2
217900513
217900628
−
—


 


hsa-mir-6808
MI0022653
—
chr1
1339650
1339708
−
—


 


hsa-mir-6807
MI0022652
8
chr19
58550285
58550376
+
—


 


hsa-mir-6806
MI0022651
—
chr19
58334688
58334751
+
—


 


hsa-mir-6805
MI0022650
28
chr19
55388181
55388242
+
—


 


hsa-mir-6804
MI0022649
—
chr19
55230885
55230952
−
—


 


hsa-mir-6803
MI0022648
9
chr19
55245186
55245250
−
—


 


hsa-mir-6802
MI0022647
—
chr19
55239912
55239976
−
—


 


hsa-mir-6801
MI0022646
—
chr19
52222020
52222098
+
—


 


hsa-mir-6800
MI0022645
1
chr19
49832018
49832099
+
—


 


hsa-mir-6799
MI0022644
—
chr19
49791866
49791934
+
—


 


hsa-mir-6798
MI0022643
—
chr19
49009906
49009972
+
—


 


hsa-mir-6797
MI0022642
6
chr19
41869627
41869698
+
—


 


hsa-mir-6796
MI0022641
2
chr19
40369846
40369907
+
—


 


hsa-mir-6795
MI0022640
3
chr19
15179283
15179350
−
—


 


hsa-mir-6794
MI0022639
26
chr19
12852260
12852327
+
—


 


hsa-mir-6793
MI0022638
—
chr19
10828973
10829035
+
—


 


hsa-mir-6792
MI0022637
7
chr19
7617439
7617505
+
—


 


hsa-mir-6791
MI0022636
5
chr19
6736712
6736778
−
—


 


hsa-mir-6790
MI0022635
—
chr19
6392921
6392983
−
—


 


hsa-mir-6789
MI0022634
51
chr19
2235829
2235926
−
—


 


hsa-mir-6788
MI0022633
65
chr18
10759584
10759649
−
—


 


hsa-mir-6787
MI0022632
—
chr17
82236668
82236728
+
—


 


hsa-mir-6786
MI0022631
13
chr17
81693757
81693869
+
—


 


hsa-mir-6785
MI0022630
2
chr17
75498548
75498628
+
—


 


hsa-mir-6784
MI0022629
—
chr17
45114367
45114433
−
—


 


hsa-mir-6783
MI0022628
—
chr17
44934618
44934681
−
—


 


hsa-mir-6782
MI0022627
—
chr17
44207771
44207839
−
—


 


hsa-mir-6781
MI0022626
—
chr17
42823880
42823943
−
—


 


hsa-mir-6780b
MI0022681
—
chr6
43434542
43434620
+
—


 


hsa-mir-6780a
MI0022625
—
chr17
42708084
42708151
−
—


 


hsa-mir-6779
MI0022624
6
chr17
38914979
38915042
+
—


 


hsa-mir-6778
MI0022623
5
chr17
18340814
18340886
−
—


 


hsa-mir-6777
MI0022622
—
chr17
17813480
17813545
−
—


 


hsa-mir-6776
MI0022621
—
chr17
2692861
2692919
−
—


 


hsa-mir-6775
MI0022620
—
chr16
87834592
87834660
−
—


 


hsa-mir-6774
MI0022619
—
chr16
85918347
85918416
+
—


 


hsa-mir-6773
MI0022618
—
chr16
68233426
68233499
−
—


 


hsa-mir-6772
MI0022617
—
chr16
57772289
57772352
−
—


 


hsa-mir-6771
MI0022616
5
chr16
50292616
50292675
+
—


 


hsa-mir-6770-3
MI0026419
—
chr16
18379351
18379410
−
✓


 


hsa-mir-6770-2
MI0026418
—
chr16
16329305
16329364
+
✓


 


hsa-mir-6770-1
MI0022615
—
chr16
14930820
14930879
+
✓


 


hsa-mir-6769b
MI0022706
—
chr1
206474803
206474864
+
—


 


hsa-mir-6769a
MI0022614
—
chr16
4671318
4671390
+
—


 


hsa-mir-6768
MI0022613
—
chr16
2463967
2464038
+
—


 


hsa-mir-6767
MI0022612
—
chr16
2445392
2445457
+
—


 


hsa-mir-6766
MI0022611
—
chr15
89326739
89326810
−
—


 


hsa-mir-6765
MI0022610
9
chr14
105150778
105150864
−
—


 


hsa-mir-6764
MI0022609
2
chr14
100277357
100277417
+
—


 


hsa-mir-6763
MI0022608
5
chr12
132581997
132582061
+
—


 


hsa-mir-6762
MI0022607
—
chr12
113291523
113291608
+
—


 


hsa-mir-6761
MI0022606
1
chr12
111799834
111799905
+
—


 


hsa-mir-6760
MI0022605
—
chr12
111304142
111304209
+
—


 


hsa-mir-676
MI0016436
—
chrX
70022857
70022923
+
✓


 


hsa-mir-6759
MI0022604
—
chr12
57748618
57748682
−
—


 


hsa-mir-6758
MI0022603
—
chr12
57512688
57512750
+
✓


 


hsa-mir-6757
MI0022602
3
chr12
53056944
53057012
+
—


 


hsa-mir-6756
MI0022601
21
chr11
119312950
119313012
−
—


 


hsa-mir-6755
MI0022600
—
chr11
86278333
86278398
+
—


 


hsa-mir-6754
MI0022599
—
chr11
71473503
71473568
+
—


 


hsa-mir-6753
MI0022598
13
chr11
68044794
68044957
+
—


 


hsa-mir-6752
MI0022597
17
chr11
67490245
67490315
+
—


 


hsa-mir-6751
MI0022596
41
chr11
65129916
65129978
−
—


 


hsa-mir-6750
MI0022595
2
chr11
64898363
64898437
−
—


 


hsa-mir-675
MI0005416
20
chr11
1996759
1996831
−
✓


 


hsa-mir-6749
MI0022594
7
chr11
64902387
64902455
−
—


 


hsa-mir-6748
MI0022593
—
chr11
62789815
62789885
+
—


 


hsa-mir-6747
MI0022592
5
chr11
62567011
62567071
−
—


 


hsa-mir-6746
MI0022591
2
chr11
61878216
61878278
−
—


 


hsa-mir-6745
MI0022590
—
chr11
47179611
47179737
−
—


 


hsa-mir-6744
MI0022589
—
chr11
1256605
1256670
+
—


 


hsa-mir-6743
MI0022588
—
chr11
209336
209406
+
—


 


hsa-mir-6742
MI0022587
—
chr1
228397048
228397109
−
—


 


hsa-mir-6741
MI0022586
—
chr1
225922080
225922142
−
—


 


hsa-mir-6740
MI0022585
4
chr1
202003124
202003236
+
—


 


hsa-mir-6739
MI0022584
—
chr1
201863373
201863447
+
—


 


hsa-mir-6738
MI0022583
—
chr1
155951273
155951336
−
—


 


hsa-mir-6737
MI0022582
—
chr1
153962351
153962420
−
—


 


hsa-mir-6736
MI0022581
6
chr1
145850587
145850645
−
—


 


hsa-mir-6735
MI0022580
1
chr1
43448539
43448611
+
—


 


hsa-mir-6734
MI0022579
1
chr1
43364648
43364715
−
—


 


hsa-mir-6733
MI0022578
—
chr1
43171652
43171712
−
—


 


hsa-mir-6732
MI0022577
2
chr1
37480230
37480289
+
—


 


hsa-mir-6731
MI0022576
2
chr1
24919345
24919416
−
—


 


hsa-mir-6730
MI0022575
51
chr1
12578957
12579023
−
—


 


hsa-mir-6729
MI0022574
—
chr1
12029158
12029222
+
—


 


hsa-mir-6728
MI0022573
84
chr1
8866502
8866590
−
—


 


hsa-mir-6727
MI0022572
5
chr1
1312502
1312566
−
—


 


hsa-mir-6726
MI0022571
—
chr1
1296110
1296170
−
—


 


hsa-mir-6724-4
MI0031518
1
chr21
8432530
8432621
+
—


 


hsa-mir-6724-3
MI0031517
1
chr21
8388362
8388453
+
—


 


hsa-mir-6724-2
MI0031516
1
chr21
8249505
8249596
+
—


 


hsa-mir-6724-1
MI0022559
1
chr21
8205315
8205406
+
—


 


hsa-mir-6722
MI0022557
—
chr9
136746893
136746970
−
—


 


hsa-mir-6721
MI0022556
2
chr6
32170030
32170116
−
—


 


hsa-mir-6720
MI0022555
1
chr6
1390314
1390411
−
✓


 


hsa-mir-6719
MI0022554
33
chr19
39829716
39829802
−
—


 


hsa-mir-6718
MI0022553
—
chr18
3885353
3885432
+
—


 


hsa-mir-6717
MI0022551
—
chr14
21023314
21023386
−
—


 


hsa-mir-6716
MI0022550
13
chr11
118644000
118644079
+
—


 


hsa-mir-6715b
MI0022549
—
chr10
112299612
112299688
−
—


 


hsa-mir-6715a
MI0022548
24
chr10
112299612
112299690
+
—


 


hsa-mir-671
MI0003760
77
chr7
151238421
151238538
+
✓


 


hsa-mir-670
MI0003933
—
chr11
43559656
43559753
+
—


 


hsa-mir-668
MI0003761
7
chr14
101055258
101055323
+
—


 


hsa-mir-665
MI0005563
14
chr14
100875033
100875104
+
—


 


hsa-mir-664b
MI0019134
2
chrX
154768596
154768656
+
✓


 


hsa-mir-664a
MI0006442
64
chr1
220200538
220200619
−
✓


 


hsa-mir-663b
MI0006336
9
chr2
132256966
132257080
−
—


 


hsa-mir-663a
MI0003672
10
chr20
26208186
26208278
−
—


 


hsa-mir-662
MI0003670
—
chr16
770183
770277
+
—


 


hsa-mir-661
MI0003669
130
chr8
143945191
143945279
−
—


 


hsa-mir-660
MI0003684
510
chrX
50013241
50013337
+
✓


 


hsa-mir-659
MI0003683
1
chr22
37847678
37847774
−
—


 


hsa-mir-658
MI0003682
1
chr22
37844272
37844371
−
—


 


hsa-mir-657
MI0003681
7
chr17
81125276
81125373
−
—


 


hsa-mir-656
MI0003678
—
chr14
101066724
101066801
+
—


 


hsa-mir-655
MI0003677
6
chr14
101049550
101049646
+
—


 


hsa-mir-654
MI0003676
5
chr14
101040219
101040299
+
✓


 


hsa-mir-653
MI0003674
—
chr7
93482760
93482855
−
✓


 


hsa-mir-6529
MI0039501
—
chr3
120084564
120084648
−
—


 


hsa-mir-652
MI0003667
189
chrX
110055329
110055426
+
✓


 


hsa-mir-6516
MI0025513
2
chr17
77089417
77089497
+
—


 


hsa-mir-6515
MI0022227
4
chr19
12940484
12940540
+
—


 


hsa-mir-6514
MI0022226
—
chr11
62792702
62792771
−
—


 


hsa-mir-6513
MI0022225
2
chr2
218280125
218280188
−
—


 


hsa-mir-6512
MI0022224
2
chr2
177313806
177313882
−
—


 


hsa-mir-6511b-2
MI0023431
6
chr16
15134075
15134145
−
—


 


hsa-mir-6511b-1
MI0022552
5
chr16
2106669
2106753
−
—


 


hsa-mir-6511a-4
MI0023566
3
chr16
18344013
18344079
−
✓


 


hsa-mir-6511a-3
MI0023565
3
chr16
16368876
16368942
+
✓


 


hsa-mir-6511a-2
MI0023564
3
chr16
16324588
16324654
+
✓


 


hsa-mir-6511a-1
MI0022223
3
chr16
14925937
14926003
+
✓


 


hsa-mir-6510
MI0022222
5
chr17
41517164
41517217
−
—


 


hsa-mir-651
MI0003666
7
chrX
8126965
8127061
+
✓


 


hsa-mir-6509
MI0022221
—
chr7
135206994
135207078
−
—


 


hsa-mir-6508
MI0022220
—
chr21
39447010
39447069
+
—


 


hsa-mir-6507
MI0022219
2
chr10
98924499
98924568
−
—


 


hsa-mir-6506
MI0022218
—
chr16
15611030
15611095
−
—


 


hsa-mir-6505
MI0022217
1
chr12
48132797
48132867
+
—


 


hsa-mir-6504
MI0022216
—
chr16
81611348
81611408
+
—


 


hsa-mir-6503
MI0022215
—
chr11
60209071
60209156
−
—


 


hsa-mir-6502
MI0022214
—
chr12
66251082
66251157
+
—


 


hsa-mir-6501
MI0022213
—
chr21
33550662
33550728
+
—


 


hsa-mir-6500
MI0022211
58
chr1
51060018
51060103
+
—


 


hsa-mir-650
MI0003665
—
chr22
22822776
22822871
+
—


 


hsa-mir-6499
MI0022209
7
chr5
151522087
151522148
−
—


 


hsa-mir-649
MI0003664
—
chr22
21034176
21034272
−
—


 


hsa-mir-648
MI0003663
31
chr22
17980868
17980961
−
—


 


hsa-mir-647
MI0003662
42
chr20
63942631
63942726
−
—


 


hsa-mir-646
MI0003661
—
chr20
60308474
60308567
+
—


 


hsa-mir-645
MI0003660
21
chr20
50585786
50585879
+
—


 


hsa-mir-644a
MI0003659
—
chr20
34466325
34466418
+
—


 


hsa-mir-643
MI0003658
216
chr19
52281797
52281893
+
✓


 


hsa-mir-642b
MI0016685
—
chr19
45674932
45675008
−
✓


 


hsa-mir-642a
MI0003657
—
chr19
45674928
45675024
+
✓


 


hsa-mir-641
MI0003656
4
chr19
40282543
40282641
−
—


 


hsa-mir-640
MI0003655
36
chr19
19435063
19435158
+
—


 


hsa-mir-639
MI0003654
78
chr19
14529543
14529640
+
—


 


hsa-mir-638
MI0003653
9
chr19
10718404
10718503
+
—


 


hsa-mir-637
MI0003652
37
chr19
3961414
3961512
−
—


 


hsa-mir-636
MI0003651
37
chr17
76736450
76736548
−
—


 


hsa-mir-635
MI0003650
—
chr17
68424451
68424548
−
—


 


hsa-mir-634
MI0003649
—
chr17
66787072
66787168
+
—


 


hsa-mir-633
MI0003648
—
chr17
62944215
62944312
+
—


 


hsa-mir-632
MI0003647
10
chr17
32350109
32350202
+
—


 


hsa-mir-631
MI0003645
—
chr15
75353611
75353685
−
—


 


hsa-mir-630
MI0003644
3442
chr15
72587217
72587313
+
—


 


hsa-mir-629
MI0003643
99
chr15
70079372
70079468
−
✓


 


hsa-mir-628
MI0003642
4
chr15
55372940
55373034
−
—


 


hsa-mir-627
MI0003641
3
chr15
42199570
42199666
−
✓


 


hsa-mir-626
MI0003640
—
chr15
41691585
41691678
+
—


 


hsa-mir-625
MI0003639
24
chr14
65471102
65471186
+
✓


 


hsa-mir-624
MI0003638
—
chr14
31014646
31014742
−
—


 


hsa-mir-623
MI0003637
—
chr13
99356131
99356228
+
—


 


hsa-mir-622
MI0003636
5
chr13
90231182
90231277
+
—


 


hsa-mir-621
MI0003635
11
chr13
40810766
40810861
+
—


 


hsa-mir-620
MI0003634
20
chr12
116148560
116148654
−
—


 


hsa-mir-619
MI0003633
201
chr12
108836908
108837006
−
—


 


hsa-mir-618
MI0003632
13
chr12
80935736
80935833
−
—


 


hsa-mir-617
MI0003631
38
chr12
80832533
80832629
−
—


 


hsa-mir-6165
MI0021472
39
chr17
49510817
49510900
+
—


 


hsa-mir-616
MI0003629
1
chr12
57519163
57519259
−
✓


 


hsa-mir-615
MI0003628
117
chr12
54033950
54034045
+
✓


 


hsa-mir-614
MI0003627
—
chr12
12915829
12915918
+
—


 


hsa-mir-6134
MI0021279
38
chrX
28495555
28495663
−
—


 


hsa-mir-6133
MI0021278
1
chr7
133290881
133290988
+
—


 


hsa-mir-6132
MI0021277
—
chr7
117020211
117020319
+
—


 


hsa-mir-6131
MI0021276
—
chr5
10478037
10478145
+
—


 


hsa-mir-6130
MI0021275
335
chr21
23079284
23079392
+
—


 


hsa-mir-613
MI0003626
24
chr12
12764649
12764743
+
—


 


hsa-mir-6129
MI0021274
8
chr17
49288346
49288454
−
—


 


hsa-mir-6128
MI0021272
20
chr11
56743873
56743981
+
—


 


hsa-mir-6127
MI0021271
87
chr1
22633258
22633366
−
—


 


hsa-mir-6126
MI0021260
68
chr16
3485381
3485469
−
—


 


hsa-mir-6125
MI0021259
4
chr12
62260359
62260454
+
—


 


hsa-mir-6124
MI0021258
214
chr11
12163683
12163767
+
—


 


hsa-mir-612
MI0003625
45
chr11
65444458
65444557
+
—


 


hsa-mir-611
MI0003624
4
chr11
61792495
61792561
−
—


 


hsa-mir-610
MI0003623
13
chr11
28056815
28056910
+
—


 


hsa-mir-6090
MI0020367
—
chr11
128522390
128522449
+
—


 


hsa-mir-609
MI0003622
—
chr10
104218789
104218883
−
—


 


hsa-mir-6089-2
MI0023563
2
chrY
2609191
2609254
+
—


 


hsa-mir-6089-1
MI0020366
2
chrX
2609191
2609254
+
—


 


hsa-mir-6088
MI0020365
24
chr19
45436654
45436704
+
—


 


hsa-mir-6086
MI0020363
83
chrX
13590292
13590346
+
—


 


hsa-mir-6085
MI0020362
—
chr15
62343029
62343138
+
—


 


hsa-mir-6084
MI0020361
—
chr1
20633679
20633788
+
—


 


hsa-mir-6083
MI0020360
6
chr3
124374332
124374437
+
—


 


hsa-mir-6082
MI0020359
22
chr4
171186184
171186292
+
—


 


hsa-mir-6081
MI0020358
—
chr9
95065350
95065446
+
—


 


hsa-mir-6080
MI0020357
—
chr17
64780759
64780824
+
—


 


hsa-mir-608
MI0003621
—
chr10
100974985
100975084
+
—


 


hsa-mir-6079
MI0020356
—
chr1
43838622
43838683
+
—


 


hsa-mir-6078
MI0020355
—
chr10
3991160
3991259
+
—


 


hsa-mir-6077
MI0020354
—
chr1
148388282
148388363
+
—


 


hsa-mir-6076
MI0020353
21
chr14
49966399
49966511
+
—


 


hsa-mir-6075
MI0020352
24
chr5
1510762
1510856
−
—


 


hsa-mir-6074
MI0020351
49
chr12
66023620
66023726
−
—


 


hsa-mir-6073
MI0020350
4
chr11
15969533
15969621
−
—


 


hsa-mir-6072
MI0020349
—
chr10
2076019
2076089
−
—


 


hsa-mir-6071
MI0020348
—
chr2
85783600
85783677
−
—


 


hsa-mir-6070
MI0020347
—
chr21
43609887
43609989
−
—


 


hsa-mir-607
MI0003620
—
chr10
96828669
96828764
−
—


 


hsa-mir-6069
MI0020346
244
chr22
35336721
35336799
−
—


 


hsa-mir-6068
MI0020345
—
chr1
63326925
63326984
−
—


 


hsa-mir-606
MI0003619
—
chr10
75552458
75552553
+
—


 


hsa-mir-605
MI0003618
20
chr10
51299573
51299655
+
✓


 


hsa-mir-604
MI0003617
—
chr10
29545004
29545097
−
—


 


hsa-mir-603
MI0003616
1
chr10
24275685
24275781
+
—


 


hsa-mir-602
MI0003615
4
chr9
137838419
137838516
+
—


 


hsa-mir-601
MI0003614
—
chr9
123402525
123402603
−
—


 


hsa-mir-600
MI0003613
—
chr9
123111546
123111643
−
—


 


hsa-mir-599
MI0003611
3
chr8
99536636
99536730
−
—


 


hsa-mir-598
MI0003610
138
chr8
11035206
11035302
−
✓


 


hsa-mir-597
MI0003609
—
chr8
9741672
9741768
+
✓


 


hsa-mir-596
MI0003608
—
chr8
1817231
1817307
+
—


 


hsa-mir-595
MI0003607
—
chr7
158532718
158532813
−
—


 


hsa-mir-593
MI0003605
—
chr7
128081861
128081960
+
—


 


hsa-mir-592
MI0003604
—
chr7
127058088
127058184
−
—


 


hsa-mir-591
MI0003603
—
chr7
96219662
96219756
−
—


 


hsa-mir-590
MI0003602
270
chr7
74191198
74191294
+
✓


 


hsa-mir-589
MI0003599
8
chr7
5495819
5495917
−
✓


 


hsa-mir-588
MI0003597
4
chr6
126484631
126484713
+
—


 


hsa-mir-587
MI0003595
—
chr6
106784125
106784220
+
—


 


hsa-mir-586
MI0003594
—
chr6
45197674
45197770
−
—


 


hsa-mir-585
MI0003592
5
chr5
169263601
169263694
−
✓


 


hsa-mir-584
MI0003591
97
chr5
149062313
149062409
−
✓


 


hsa-mir-583
MI0003590
—
chr5
96079138
96079212
+
—


 


hsa-mir-582
MI0003589
151
chr5
59703606
59703703
−
✓


 


hsa-mir-581
MI0003588
3
chr5
53951504
53951599
−
—


 


hsa-mir-580
MI0003587
—
chr5
36147892
36147988
−
—


 


hsa-mir-579
MI0003586
4
chr5
32394378
32394475
−
✓


 


hsa-mir-5787
MI0019797
—
chr3
50227436
50227490
+
—


 


hsa-mir-578
MI0003585
—
chr4
165386242
165386337
+
—


 


hsa-mir-577
MI0003584
13
chr4
114656759
114656854
+
✓


 


hsa-mir-576
MI0003583
15
chr4
109488698
109488795
+
✓


 


hsa-mir-575
MI0003582
81
chr4
82753337
82753430
−
—


 


hsa-mir-574
MI0003581
780
chr4
38868032
38868127
+
✓


 


hsa-mir-5739
MI0019412
50
chr22
28459869
28459948
+
—


 


hsa-mir-573
MI0003580
—
chr4
24520192
24520290
−
—


 


hsa-mir-572
MI0003579
6
chr4
11368827
11368921
+
—


 


hsa-mir-571
MI0003578
—
chr4
350157
350252
+
—


 


hsa-mir-5708
MI0019316
55
chr8
80241389
80241473
+
—


 


hsa-mir-5707
MI0019315
—
chr7
158591616
158591696
+
—


 


hsa-mir-5706
MI0019314
—
chr5
119154637
119154716
+
—


 


hsa-mir-5705
MI0019313
—
chr4
87300495
87300583
−
—


 


hsa-mir-5704
MI0019312
—
chr3
131985855
131985931
−
—


 


hsa-mir-5703
MI0019310
6
chr2
227472132
227472187
+
—


 


hsa-mir-5702
MI0019309
—
chr2
226658710
226658793
−
—


 


hsa-mir-5701-3
MI0031522
—
chr15
21951242
21951323
+
—


 


hsa-mir-5701-2
MI0019593
—
chr15
21513959
21514040
+
—


 


hsa-mir-5701-1
MI0019308
—
chr15
20940252
20940333
+
—


 


hsa-mir-5700
MI0019307
—
chr12
94561789
94561859
+
—


 


hsa-mir-570
MI0003577
4
chr3
195699401
195699497
+
—


 


hsa-mir-5699
MI0019306
6
chr10
641689
641778
−
—


 


hsa-mir-5698
MI0019305
—
chr1
154104521
154104592
−
—


 


hsa-mir-5697
MI0019304
—
chr1
9967381
9967458
+
—


 


hsa-mir-5696
MI0019303
—
chr2
101309450
101309534
+
—


 


hsa-mir-5695
MI0019302
1
chr19
12920320
12920404
+
—


 


hsa-mir-5694
MI0019301
2
chr14
67441855
67441930
−
—


 


hsa-mir-5693
MI0019300
4
chr13
51348567
51348639
−
—


 


hsa-mir-5692c-2
MI0019289
3
chr7
97964405
97964481
−
—


 


hsa-mir-5692c-1
MI0019288
—
chr5
135802985
135803075
−
—


 


hsa-mir-5692b
MI0019311
—
chr21
42950928
42951014
−
—


 


hsa-mir-5692a-2
MI0019298
—
chr8
12719132
12719190
+
—


 


hsa-mir-5692a-1
MI0019297
—
chr7
97963658
97963726
+
—


 


hsa-mir-5691
MI0019296
—
chr11
9090312
9090379
−
—


 


hsa-mir-5690
MI0019295
—
chr6
35664717
35664789
−
—


 


hsa-mir-569
MI0003576
—
chr3
171106664
171106759
−
—


 


hsa-mir-5689
MI0019294
9
chr6
10439717
10439794
+
—


 


hsa-mir-5688
MI0019292
—
chr3
85385710
85385792
+
—


 


hsa-mir-5687
MI0019291
—
chr5
55508850
55508926
−
—


 


hsa-mir-5685
MI0019287
21
chr6
53276993
53277071
+
—


 


hsa-mir-5684
MI0019285
46
chr19
12787128
12787192
+
—


 


hsa-mir-5683
MI0019284
—
chr6
6169334
6169409
+
—


 


hsa-mir-5682
MI0019282
—
chr3
121049640
121049715
+
✓


 


hsa-mir-5681b
MI0019293
—
chr8
74548550
74548609
−
—


 


hsa-mir-5681a
MI0019281
—
chr8
74548543
74548617
+
—


 


hsa-mir-5680
MI0019280
—
chr8
102125432
102125515
+
—


 


hsa-mir-568
MI0003574
—
chr3
114316475
114316569
−
—


 


hsa-mir-567
MI0003573
70
chr3
112112801
112112898
+
—


 


hsa-mir-564
MI0003570
—
chr3
44861888
44861981
+
—


 


hsa-mir-563
MI0003569
—
chr3
15873771
15873849
+
—


 


hsa-mir-562
MI0003568
—
chr2
232172653
232172747
+
—


 


hsa-mir-561
MI0003567
32
chr2
188297492
188297588
+
✓


 


hsa-mir-5591
MI0019151
—
chr4
39411910
39411974
+
—


 


hsa-mir-5590
MI0019150
—
chr2
134857820
134857873
+
—


 


hsa-mir-559
MI0003565
9
chr2
47377675
47377770
+
—


 


hsa-mir-5589
MI0019148
—
chr19
10038354
10038413
+
—


 


hsa-mir-5588
MI0019147
97
chr3
185253210
185253272
−
—


 


hsa-mir-5587
MI0019144
3
chr16
535316
535368
+
—


 


hsa-mir-5586
MI0019143
—
chr14
59646962
59647020
−
—


 


hsa-mir-5585
MI0019142
320
chr1
32086949
32087007
+
—


 


hsa-mir-5584
MI0019141
—
chr1
44545493
44545552
+
—


 


hsa-mir-5583-2
MI0019140
—
chr18
39676719
39676777
−
✓


 


hsa-mir-5583-1
MI0019139
—
chr18
39676721
39676779
+
✓


 


hsa-mir-5582
MI0019138
—
chr11
46753125
46753192
−
—


 


hsa-mir-5581
MI0019136
—
chr1
37500935
37500994
−
—


 


hsa-mir-5580
MI0019135
—
chr14
53948427
53948484
−
—


 


hsa-mir-558
MI0003564
134
chr2
32532153
32532246
+
—


 


hsa-mir-5579
MI0019133
—
chr11
79422169
79422226
−
—


 


hsa-mir-5572
MI0019117
94
chr15
80581103
80581239
+
—


 


hsa-mir-5571
MI0019115
22
chr22
22886267
22886379
+
—


 


hsa-mir-557
MI0003563
—
chr1
168375524
168375621
+
—


 


hsa-mir-556
MI0003562
3
chr1
162342546
162342640
+
✓


 


hsa-mir-555
MI0003561
—
chr1
155346350
155346445
−
—


 


hsa-mir-554
MI0003559
—
chr1
151545796
151545891
+
—


 


hsa-mir-553
MI0003558
—
chr1
100281241
100281308
+
—


 


hsa-mir-552
MI0003557
—
chr1
34669599
34669694
−
✓


 


hsa-mir-551b
MI0003575
70
chr3
168551854
168551949
+
✓


 


hsa-mir-551a
MI0003556
7
chr1
3560695
3560790
−
—


 


hsa-mir-550b-2
MI0016687
2
chr7
32732981
32733077
−
—


 


hsa-mir-550b-1
MI0016686
2
chr7
30289794
30289890
−
—


 


hsa-mir-550a-3
MI0003762
16
chr7
29680734
29680828
−
✓


 


hsa-mir-550a-2
MI0003601
16
chr7
32732981
32733077
+
✓


 


hsa-mir-550a-1
MI0003600
16
chr7
30289794
30289890
+
✓


 


hsa-mir-549a
MI0003679
—
chr15
80841978
80842073
−
—


 


hsa-mir-548z
MI0016688
3
chr12
64622509
64622605
−
—


 


hsa-mir-548y
MI0016595
1
chr14
47760995
47761104
−
✓


 


hsa-mir-548x-2
MI0016833
8
chr13
65966330
65966429
−
—


 


hsa-mir-548x
MI0014244
3
chr21
18686090
18686164
−
✓


 


hsa-mir-548w
MI0014222
3
chr16
26025237
26025310
+
—


 


hsa-mir-548v
MI0014174
9
chr8
17681578
17681657
−
✓


 


hsa-mir-548u
MI0014168
17
chr6
57390132
57390212
+
—


 


hsa-mir-548t
MI0014164
2
chr4
173268160
173268233
+
—


 


hsa-mir-548s
MI0014141
5
chr2
11767444
11767525
+
—


 


hsa-mir-548q
MI0010637
5
chr10
12725254
12725353
−
—


 


hsa-mir-548p
MI0006420
7
chr5
100816482
100816565
−
—


 


hsa-mir-548o-2
MI0016746
15
chr20
38516563
38516632
+
✓


 


hsa-mir-548o
MI0006402
5
chr7
102405742
102405855
−
—


 


hsa-mir-548n
MI0006399
7
chr7
34940760
34940834
−
—


 


hsa-mir-548m
MI0006400
—
chrX
95063141
95063226
−
—


 


hsa-mir-5481
MI0006361
8
chr11
94466495
94466580
−
—


 


hsa-mir-548k
MI0006354
15
chr11
70283955
70284070
+
✓


 


hsa-mir-548j
MI0006345
1
chr22
26555212
26555323
−
✓


 


hsa-mir-5481-4
MI0006424
16
chrX
84225752
84225828
−
—


 


hsa-mir-5481-3
MI0006423
17
chr8
8088941
8089089
−
—


 


hsa-mir-5481-2
MI0006422
17
chr4
9556168
9556316
−
—


 


hsa-mir-5481-1
MI0006421
17
chr3
125790404
125790552
−
—


 


hsa-mir-548h-5
MI0016751
2
chr6
131792172
131792231
+
—


 


hsa-mir-548h-4
MI0006414
4
chr8
27048853
27048963
−
✓


 


hsa-mir-548h-3
MI0006413
6
chr17
13543529
13543646
−
✓


 


hsa-mir-548h-2
MI0006412
3
chr16
11306440
11306527
−
✓


 


hsa-mir-548h-1
MI0006411
2
chr14
64095024
64095125
−
—


 


hsa-mir-548g
MI0006395
—
chr4
147344629
147344717
−
—


 


hsa-mir-548f-5
MI0006378
—
chrX
32641474
32641559
−
—


 


hsa-mir-548f-4
MI0006377
8
chr7
147378017
147378121
−
—


 


hsa-mir-548f-3
MI0006376
3
chr5
110513829
110513915
−
—


 


hsa-mir-548f-2
MI0006375
2
chr2
212426263
212426360
−
—


 


hsa-mir-548f-1
MI0006374
3
chr10
54607874
54607957
−
—


 


hsa-mir-548e
MI0006344
14
chr10
110988926
110989013
+
✓


 


hsa-mir-548d-2
MI0003671
15
chr17
67471489
67471585
−
✓


 


hsa-mir-548d-1
MI0003668
12
chr8
123348034
123348130
−
✓


 


hsa-mir-548c
MI0003630
13
chr12
64622509
64622605
+
—


 


hsa-mir-548bc
MI0032315
1
chr17
39297400
39297509
+
—


 


hsa-mir-548bb
MI0029321
—
chr3
60617805
60617870
−
—


 


hsa-mir-548ba
MI0025747
8
chr2
49059603
49059658
+
—


 


hsa-mir-548b
MI0003596
35
chr6
119069047
119069143
−
✓


 


hsa-mir-548az
MI0022212
8
chr8
119325171
119325265
+
—


 


hsa-mir-548ay
MI0022210
8
chr3
32506283
32506389
−
✓


 


hsa-mir-548ax
MI0019286
1
chrX
11318614
11318686
−
—


 


hsa-mir-548aw
MI0019283
—
chr9
132945707
132945771
+
—


 


hsa-mir-548av
MI0019152
25
chr18
72853321
72853382
−
—


 


hsa-mir-548au
MI0019145
8
chr9
93594841
93594894
+
—


 


hsa-mir-548at
MI0019137
—
chr17
42494773
42494830
+
✓


 


hsa-mir-548as
MI0019132
1
chr13
92490163
92490220
+
—


 


hsa-mir-548ar
MI0019131
—
chr13
114244505
114244561
+
✓


 


hsa-mir-548aq
MI0019130
3
chr3
185767847
185767904
−
✓


 


hsa-mir-548ap
MI0017875
2
chr15
85825635
85825730
+
✓


 


hsa-mir-548ao
MI0017871
—
chr8
41271048
41271143
−
—


 


hsa-mir-548an
MI0016907
—
chrX
106639814
106639896
+
✓


 


hsa-mir-548am
MI0016904
16
chrX
16627012
16627085
−
✓


 


hsa-mir-548a1
MI0016851
1
chr11
74399237
74399333
+
—


 


hsa-mir-548ak
MI0016840
3
chr10
12130760
12130816
−
—


 


hsa-mir-548aj-2
MI0016815
2
chrX
38023895
38023986
−
—


 


hsa-mir-548aj-1
MI0016814
2
chr6
132115192
132115263
−
—


 


hsa-mir-548a1
MI0016813
—
chr6
99124609
99124696
+
—


 


hsa-mir-548ah
MI0016796
7
chr4
76575551
76575626
+
—


 


hsa-mir-548ag-2
MI0016794
4
chr20
60564562
60564625
+
✓


 


hsa-mir-548ag-1
MI0016793
3
chr4
60922619
60922684
+
—


 


hsa-mir-548ae-2
MI0016780
10
chr5
58530043
58530109
−
✓


 


hsa-mir-548ae-1
MI0016779
15
chr2
184378975
184379044
+
—


 


hsa-mir-548ad
MI0016770
8
chr2
35471405
35471486
+
—


 


hsa-mir-548ac
MI0016762
6
chr1
116560024
116560111
−
—


 


hsa-mir-548ab
MI0016752
7
chr3
103524033
103524116
−
—


 


hsa-mir-548aa-2
MI0016690
1
chr17
67471489
67471585
+
—


 


hsa-mir-548aa-1
MI0016689
1
chr8
123348034
123348130
+
—


 


hsa-mir-548a-3
MI0003612
18
chr8
104484369
104484465
−
✓


 


hsa-mir-548a-2
MI0003598
25
chr6
135239160
135239256
+
—


 


hsa-mir-548a-1
MI0003593
21
chr6
18571784
18571880
+
—


 


hsa-mir-545
MI0003516
11
chrX
74287104
74287209
−
✓


 


hsa-mir-544b
MI0014159
1
chr3
124732439
124732516
+
—


 


hsa-mir-544a
MI0003515
2
chr14
101048658
101048748
+
—


 


hsa-mir-543
MI0005565
6
chr14
101031987
101032064
+
—


 


hsa-mir-542
MI0003686
174
chrX
134541341
134541437
−
✓


 


hsa-mir-541
MI0005539
28
chr14
101064495
101064578
+
✓


 


hsa-mir-539
MI0003514
4
chr14
101047321
101047398
+
✓


 


hsa-mir-532
MI0003205
322
chrX
50003148
50003238
+
✓


 


hsa-mir-527
MI0003179
27
chr19
53754018
53754102
+
—


 


hsa-mir-526b
MI0003150
1
chr19
53694393
53694475
+
—


 


hsa-mir-526a-2
MI0003168
—
chr19
53726922
53726986
+
—


 


hsa-mir-526a-1
MI0003157
23
chr19
53706252
53706336
+
—


 


hsa-mir-525
MI0003152
8
chr19
53697533
53697617
+
—


 


hsa-mir-524
MI0003160
2
chr19
53711002
53711088
+
—


 


hsa-mir-523
MI0003153
3
chr19
53698385
53698471
+
—


 


hsa-mir-522
MI0003177
8
chr19
53751211
53751297
+
✓


 


hsa-mir-521-2
MI0003163
13
chr19
53716594
53716680
+
—


 


hsa-mir-521-1
MI0003176
—
chr19
53748636
53748722
+
—


 


hsa-mir-520h
MI0003175
18
chr19
53742512
53742599
+
—


 


hsa-mir-520g
MI0003166
17
chr19
53722166
53722255
+
—


 


hsa-mir-520f
MI0003146
4
chr19
53682159
53682245
+
—


 


hsa-mir-520e
MI0003143
33
chr19
53675711
53675797
+
—


 


hsa-mir-520d
MI0003164
—
chr19
53720096
53720182
+
—


 


hsa-mir-520c
MI0003158
6
chr19
53707453
53707539
+
✓


 


hsa-mir-520b
MI0003155
9
chr19
53701227
53701287
+
—


 


hsa-mir-520a
MI0003149
4
chr19
53690881
53690965
+
✓


 


hsa-mir-519e
MI0003145
10
chr19
53679940
53680023
+
—


 


hsa-mir-519d
MI0003162
3
chr19
53713347
53713434
+
—


 


hsa-mir-519c
MI0003148
14
chr19
53686469
53686555
+
—


 


hsa-mir-519b
MI0003151
8
chr19
53695213
53695293
+
✓


 


hsa-mir-519a-2
MI0003182
8
chr19
53762344
53762430
+
✓


 


hsa-mir-519a-1
MI0003178
11
chr19
53752397
53752481
+
✓


 


hsa-mir-5197
MI0018176
—
chr5
143679860
143679971
+
—


 


hsa-mir-5196
MI0018175
20
chr19
35345513
35345627
+
—


 


hsa-mir-5195
MI0018174
—
chr14
106850885
106850999
−
—


 


hsa-mir-5194
MI0018173
—
chr8
130008334
130008453
−
—


 


hsa-mir-5193
MI0018172
2
chr3
49806137
49806245
−
—


 


hsa-mir-5192
MI0018171
4
chr2
62205826
62205917
+
—


 


hsa-mir-5191
MI0018170
64
chr1
201719508
201719627
+
—


 


hsa-mir-5190
MI0018169
—
chr18
13459947
13460026
+
—


 


hsa-mir-518f
MI0003154
6
chr19
53700015
53700101
+
—


 


hsa-mir-518e
MI0003169
3
chr19
53729838
53729925
+
—


 


hsa-mir-518d
MI0003171
—
chr19
53734877
53734963
+
—


 


hsa-mir-518c
MI0003159
5
chr19
53708735
53708835
+
—


 


hsa-mir-518b
MI0003156
6
chr19
53702737
53702819
+
—


 


hsa-mir-518a-2
MI0003173
—
chr19
53739333
53739419
+
—


 


hsa-mir-518a-1
MI0003170
—
chr19
53731006
53731090
+
—


 


hsa-mir-5189
MI0018168
—
chr16
88468918
88469031
+
—


 


hsa-mir-5188
MI0018167
—
chr12
124915547
124915659
+
—


 


hsa-mir-5187
MI0018166
1
chr1
161227186
161227261
+
—


 


hsa-mir-5186
MI0018165
13
chr3
151565876
151565995
−
—


 


hsa-mir-517c
MI0003174
1
chr19
53741313
53741407
+
—


 


hsa-mir-517b
MI0003165
1
chr19
53721076
53721142
+
—


 


hsa-mir-517a
MI0003161
1
chr19
53712268
53712354
+
—


 


hsa-mir-516b-2
MI0003167
—
chr19
53725442
53725526
+
—


 


hsa-mir-516b-1
MI0003172
—
chr19
53736845
53736934
+
—


 


hsa-mir-516a-2
MI0003181
32
chr19
53761133
53761222
+
—


 


hsa-mir-516a-1
MI0003180
31
chr19
53756741
53756830
+
—


 


hsa-mir-515-2
MI0003147
7
chr19
53685009
53685091
+
—


 


hsa-mir-515-1
MI0003144
7
chr19
53679003
53679085
+
—


 


hsa-mir-514b
MI0014251
573
chrX
147250151
147250230
−
✓


 


hsa-mir-514a-3
MI0003200
434
chrX
147284641
147284728
−
✓


 


hsa-mir-514a-2
MI0003199
434
chrX
147281943
147282030
−
✓


 


hsa-mir-514a-1
MI0003198
431
chrX
147279247
147279344
−
✓


 


hsa-mir-513c
MI0006649
23
chrX
147189704
147189787
−
✓


 


hsa-mir-513b
MI0006648
24
chrX
147199044
147199127
−
—


 


hsa-mir-513a-2
MI0003192
20
chrX
147225826
147225952
−
✓


 


hsa-mir-513a-1
MI0003191
23
chrX
147213463
147213591
−
✓


 


hsa-mir-512-2
MI0003141
—
chr19
53669157
53669254
+
✓


 


hsa-mir-512-1
MI0003140
—
chr19
53666679
53666762
+
✓


 


hsa-mir-511
MI0003127
—
chr10
17845107
17845193
+
✓


 


hsa-mir-5100
MI0019116
107
chr10
42997563
42997681
+
—


 


hsa-mir-510
MI0003197
20
chrX
147272335
147272408
−
✓


 


hsa-mir-5094
MI0017983
—
chr15
89850637
89850721
−
—


 


hsa-mir-5093
MI0017982
38
chr16
85306226
85306325
−
—


 


hsa-mir-5092
MI0017981
—
chr3
125151465
125151552
−
—


 


hsa-mir-5091
MI0017980
6
chr4
13627865
13627957
+
—


 


hsa-mir-5090
MI0017979
2
chr7
102465742
102465826
+
—


 


hsa-mir-509-3
MI0005717
352
chrX
147259652
147259726
−
✓


 


hsa-mir-509-2
MI0005530
259
chrX
147258760
147258850
−
✓


 


hsa-mir-509-1
MI0003196
259
chrX
147260532
147260625
−
✓


 


hsa-mir-5089
MI0017978
34
chr17
46973017
46973100
+
—


 


hsa-mir-5088
MI0017977
—
chr19
49682117
49682195
+
—


 


hsa-mir-5087
MI0017976
—
chr1
148334479
148334554
−
—


 


hsa-mir-508
MI0003195
347
chrX
147236913
147237027
−
✓


 


hsa-mir-507
MI0003194
20
chrX
147230984
147231077
−
—


 


hsa-mir-506
MI0003193
55
chrX
147230720
147230843
−
✓


 


hsa-mir-505
MI0003190
86
chrX
139924148
139924231
−
—


 


hsa-mir-5047
MI0017932
—
chr17
64501214
64501313
−
—


 


hsa-mir-504
MI0003189
4
chrX
138667711
138667793
−
✓


 


hsa-mir-503
MI0003188
132
chrX
134546328
134546398
−
✓


 


hsa-mir-502
MI0003186
58
chrX
50014598
50014683
+
✓


 


hsa-mir-5011
MI0017879
—
chr18
67081584
67081686
+
—


 


hsa-mir-5010
MI0017878
1
chr17
42514188
42514307
+
✓


 


hsa-mir-501
MI0003185
53
chrX
50009722
50009805
+
✓


 


hsa-mir-500b
MI0015903
13
chrX
50010672
50010750
+
—


 


hsa-mir-500a
MI0003184
63
chrX
50008431
50008514
+
✓


 


hsa-mir-5009
MI0017877
—
chr15
89883931
89884030
−
—


 


hsa-mir-5008
MI0017876
1
chr1
227941590
227941683
−
—


 


hsa-mir-5007
MI0017874
—
chr13
55174454
55174548
+
—


 


hsa-mir-5006
MI0017873
8
chr13
41568286
41568395
−
—


 


hsa-mir-5004
MI0017870
7
chr6
33438331
33438437
+
—


 


hsa-mir-5003
MI0017869
12
chr5
172662165
172662263
+
—


 


hsa-mir-5002
MI0017868
9
chr3
124132929
124133025
+
—


 


hsa-mir-5001
MI0017867
—
chr2
232550474
232550573
−
✓


 


hsa-mir-5000
MI0017866
11
chr2
75090812
75090914
+
✓


 


hsa-mir-499b
MI0017396
6
chr20
34990400
34990472
−
—


 


hsa-mir-499a
MI0003183
382
chr20
34990376
34990497
+
✓


 


hsa-mir-4999
MI0017865
13
chr19
8389290
8389380
−
—


 


hsa-mir-498
MI0003142
54
chr19
53674197
53674320
+
—


 


hsa-mir-497
MI0003138
267
chr17
7017911
7018022
−
—


 


hsa-mir-496
MI0003136
—
chr14
101060573
101060674
+
—


 


hsa-mir-495
MI0003135
91
chr14
101033755
101033836
+
✓


 


hsa-mir-494
MI0003134
10
chr14
101029634
101029714
+
—


 


hsa-mir-493
MI0003132
10
chr14
100869060
100869148
+
✓


 


hsa-mir-492
MI0003131
—
chr12
94834398
94834513
+
—


 


hsa-mir-491
MI0003126
4
chr9
20716105
20716188
+
✓


 


hsa-mir-490
MI0003125
13
chr7
136903167
136903294
+
✓


 


hsa-mir-489
MI0003124
—
chr7
93483936
93484019
−
—


 


hsa-mir-488
MI0003123
16
chr1
177029363
177029445
−
✓


 


hsa-mir-487b
MI0003530
23
chr14
101046455
101046538
+
—


 


hsa-mir-487a
MI0002471
—
chr14
101052446
101052525
+
✓


 


hsa-mir-486-2
MI0023622
645
chr8
41660444
41660507
+
✓


 


hsa-mir-486-1
MI0002470
650
chr8
41660441
41660508
−
✓


 


hsa-mir-485
MI0002469
9
chr14
101055419
101055491
+
✓


 


hsa-mir-484
MI0002468
435
chr16
15643294
15643372
+
—


 


hsa-mir-483
MI0002467
83
chr11
2134134
2134209
−
✓


 


hsa-mir-4804
MI0017452
3
chr5
72878591
72878663
+
—


 


hsa-mir-4803
MI0017451
—
chr5
72169467
72169540
+
—


 


hsa-mir-4802
MI0017450
—
chr4
40502040
40502119
−
✓


 


hsa-mir-4801
MI0017449
—
chr4
37241910
37241991
−
—


 


hsa-mir-4800
MI0017448
4
chr4
2250077
2250156
−
—


 


hsa-mir-4799
MI0017446
—
chr4
147782595
147782668
+
—


 


hsa-mir-4798
MI0017445
—
chr4
7310450
7310524
+
—


 


hsa-mir-4797
MI0017444
—
chr3
197293878
197293948
−
—


 


hsa-mir-4796
MI0017443
—
chr3
114743445
114743525
−
—


 


hsa-mir-4795
MI0017442
—
chr3
87226189
87226277
−
—


 


hsa-mir-4794
MI0017441
—
chr1
64579847
64579923
+
—


 


hsa-mir-4793
MI0017440
8
chr3
48644194
48644280
−
—


 


hsa-mir-4791
MI0017438
3
chr3
19314848
19314931
−
—


 


hsa-mir-4790
MI0017437
52
chr3
5250177
5250255
−
—


 


hsa-mir-4789
MI0017436
—
chr3
175369540
175369621
+
—


 


hsa-mir-4788
MI0017435
4
chr3
134437827
134437906
+
—


 


hsa-mir-4787
MI0017434
4
chr3
50675080
50675163
+
—


 


hsa-mir-4786
MI0017433
—
chr2
239943015
239943094
−
✓


 


hsa-mir-4785
MI0017430
—
chr2
160407810
160407882
−
—


 


hsa-mir-4784
MI0017429
—
chr2
131491160
131491236
−
—


 


hsa-mir-4783
MI0017428
—
chr2
127423537
127423618
−
—


 


hsa-mir-4782
MI0017427
—
chr2
113721290
113721368
−
—


 


hsa-mir-4781
MI0017426
—
chr1
54054079
54054154
+
—


 


hsa-mir-4780
MI0017424
—
chr2
88082519
88082599
−
—


 


hsa-mir-4779
MI0017423
11
chr2
86193026
86193108
−
—


 


hsa-mir-4778
MI0017422
2
chr2
66358249
66358328
−
✓


 


hsa-mir-4777
MI0017421
—
chr2
231362708
231362793
+
—


 


hsa-mir-4776-2
MI0017420
114
chr2
212926257
212926336
−
—


 


hsa-mir-4776-1
MI0017419
8
chr2
212926257
212926336
+
—


 


hsa-mir-4775
MI0017418
—
chr2
207754807
207754881
+
—


 


hsa-mir-4774
MI0017417
—
chr2
168582943
168583018
+
—


 


hsa-mir-4773-2
MI0017416
18
chr2
151368334
151368411
−
—


 


hsa-mir-4773-1
MI0017415
17
chr2
151368334
151368411
+
—


 


hsa-mir-4772
MI0017414
1
chr2
102432289
102432366
+
—


 


hsa-mir-4771-2
MI0017413
—
chr2
111771061
111771134
−
—


 


hsa-mir-4771-1
MI0017412
—
chr2
87194786
87194859
+
—


 


hsa-mir-4770
MI0017411
—
chrX
6383906
6383963
−
—


 


hsa-mir-4769
MI0017410
—
chrX
47587429
47587505
+
—


 


hsa-mir-4768
MI0017409
—
chrX
17425881
17425954
+
—


 


hsa-mir-4767
MI0017408
2
chrX
7147860
7147937
+
—


 


hsa-mir-4766
MI0017407
1
chr22
40813883
40813958
−
—


 


hsa-mir-4765
MI0017406
—
chr2
32635255
32635331
+
—


 


hsa-mir-4764
MI0017405
—
chr22
33436582
33436669
−
—


 


hsa-mir-4763
MI0017404
5
chr22
46113566
46113657
+
—


 


hsa-mir-4762
MI0017403
—
chr22
45760524
45760598
+
—


 


hsa-mir-4761
MI0017402
8
chr22
19963753
19963834
+
✓


 


hsa-mir-4760
MI0017401
—
chr21
40212352
40212431
−
—


 


hsa-mir-4759
MI0017400
—
chr21
26953961
26954043
+
—


 


hsa-mir-4758
MI0017399
—
chr20
62332487
62332557
−
—


 


hsa-mir-4757
MI0017398
—
chr2
19348429
19348505
+
—


 


hsa-mir-4756
MI0017397
11
chr20
54068408
54068485
−
—


 


hsa-mir-4755
MI0017395
—
chr20
34049119
34049190
+
✓


 


hsa-mir-4754
MI0017394
—
chr19
58386770
58386858
−
—


 


hsa-mir-4753
MI0017392
1
chr1
235190034
235190116
−
—


 


hsa-mir-4752
MI0017391
—
chr19
54282109
54282180
+
—


 


hsa-mir-4751
MI0017390
—
chr19
49933064
49933137
+
—


 


hsa-mir-4750
MI0017389
—
chr19
49888175
49888230
+
—


 


hsa-mir-4749
MI0017388
9
chr19
49854591
49854651
+
—


 


hsa-mir-4748
MI0017387
—
chr19
10780254
10780335
+
—


 


hsa-mir-4747
MI0017386
—
chr19
4932687
4932740
+
—


 


hsa-mir-4746
MI0017385
—
chr19
4445978
4446048
+
—


 


hsa-mir-4745
MI0017384
—
chr19
804940
805001
+
—


 


hsa-mir-4744
MI0017382
—
chr18
49049687
49049768
−
—


 


hsa-mir-4743
MI0017381
—
chr18
48670600
48670668
+
—


 


hsa-mir-4742
MI0017380
—
chr1
224398227
224398311
−
—


 


hsa-mir-4741
MI0017379
—
chr18
22933349
22933438
+
—


 


hsa-mir-4740
MI0017378
26
chr17
81400716
81400778
−
—


 


hsa-mir-4739
MI0017377
166
chr17
79707176
79707249
−
—


 


hsa-mir-4738
MI0017376
3
chr17
75784521
75784607
−
—


 


hsa-mir-4737
MI0017374
—
chr17
60043025
60043105
−
—


 


hsa-mir-4736
MI0017373
282
chr17
58335976
58336022
−
—


 


hsa-mir-4735
MI0017372
75
chr1
196582413
196582481
−
—


 


hsa-mir-4734
MI0017371
2
chr17
38702262
38702331
−
—


 


hsa-mir-4733
MI0017370
—
chr17
31094350
31094425
−
—


 


hsa-mir-4732
MI0017369
1
chr17
28861655
28861730
−
—


 


hsa-mir-4731
MI0017368
—
chr17
15251627
15251696
−
—


 


hsa-mir-4730
MI0017367
8
chr17
80419418
80419493
+
—


 


hsa-mir-4729
MI0017366
—
chr17
59366083
59366154
+
—


 


hsa-mir-4728
MI0017365
—
chr17
39726495
39726561
+
—


 


hsa-mir-4727
MI0017364
—
chr17
38825838
38825892
+
—


 


hsa-mir-4726
MI0017363
—
chr17
38719691
38719748
+
—


 


hsa-mir-4725
MI0017362
24
chr17
31575269
31575358
+
—


 


hsa-mir-4724
MI0017361
2
chr17
31534883
31534971
+
—


 


hsa-mir-4723
MI0017359
1
chr17
28360654
28360734
+
—


 


hsa-mir-4722
MI0017357
11
chr16
88716278
88716337
−
—


 


hsa-mir-4721
MI0017356
—
chr16
28843919
28844007
−
—


 


hsa-mir-4720
MI0017355
—
chr16
81385018
81385093
+
—


 


hsa-mir-4719
MI0017354
41
chr16
76868936
76869019
+
—


 


hsa-mir-4718
MI0017353
—
chr16
12720321
12720371
+
—


 


hsa-mir-4717
MI0017352
—
chr16
2274620
2274691
+
—


 


hsa-mir-4716
MI0017350
5
chr15
49169070
49169153
−
—


 


hsa-mir-4715
MI0017349
—
chr15
25848747
25848825
−
—


 


hsa-mir-4714
MI0017348
—
chr15
98784426
98784502
+
—


 


hsa-mir-4713
MI0017347
4
chr15
51242190
51242264
+
—


 


hsa-mir-4712
MI0017346
—
chr15
50360329
50360410
+
—


 


hsa-mir-4711
MI0017345
—
chr1
59733227
59733296
−
—


 


hsa-mir-4710
MI0017344
2
chr14
104677694
104677749
−
—


 


hsa-mir-4709
MI0017342
4
chr14
74480133
74480204
−
—


 


hsa-mir-4708
MI0017341
8
chr14
65335117
65335183
−
—


 


hsa-mir-4707
MI0017340
—
chr14
22956950
22957029
−
—


 


hsa-mir-4706
MI0017339
8
chr14
65044688
65044769
+
—


 


hsa-mir-4705
MI0017338
—
chr13
102045934
102046004
−
—


 


hsa-mir-4704
MI0017337
—
chr13
66218250
66218324
+
—


 


hsa-mir-4703
MI0017336
—
chr13
51552589
51552667
+
—


 


hsa-mir-4701
MI0017334
—
chr12
48771975
48772037
−
—


 


hsa-mir-4700
MI0017333
—
chr12
120723193
120723266
+
—


 


hsa-mir-4699
MI0017332
29
chr12
81158388
81158461
+
—


 


hsa-mir-4698
MI0017331
—
chr12
47187812
47187891
+
—


 


hsa-mir-4697
MI0017330
—
chr11
133898504
133898581
−
—


 


hsa-mir-4696
MI0017329
—
chr11
74720268
74720337
−
—


 


hsa-mir-4695
MI0017328
2
chr1
18883202
18883275
−
—


 


hsa-mir-4694
MI0017327
75
chr11
19760004
19760083
−
—


 


hsa-mir-4693
MI0017326
5
chr11
103849906
103849980
+
—


 


hsa-mir-4692
MI0017325
—
chr11
72783530
72783592
+
—


 


hsa-mir-4691
MI0017324
9
chr11
68033897
68033981
+
—


 


hsa-mir-4690
MI0017323
—
chr11
65636310
65636369
+
—


 


hsa-mir-4689
MI0017322
30
chr1
5862672
5862741
−
—


 


hsa-mir-4688
MI0017321
50
chr11
46376402
46376484
+
—


 


hsa-mir-4687
MI0017319
5
chr11
3856062
3856141
+
—


 


hsa-mir-4686
MI0017318
18
chr11
2173063
2173138
+
—


 


hsa-mir-4685
MI0017317
26
chr10
98431292
98431360
−
—


 


hsa-mir-4684
MI0017316
2
chr1
22719517
22719598
+
—


 


hsa-mir-4683
MI0017315
1
chr10
35641172
35641252
−
—


 


hsa-mir-4682
MI0017314
—
chr10
119958513
119958592
+
—


 


hsa-mir-4681
MI0017313
19
chr10
119377972
119378043
+
—


 


hsa-mir-4680
MI0017312
—
chr10
110898090
110898155
+
—


 


hsa-mir-4679-2
MI0017311
4
chr10
89063335
89063411
−
—


 


hsa-mir-4679-1
MI0017310
4
chr10
89063336
89063410
+
—


 


hsa-mir-4678
MI0017309
—
chr10
87503881
87503954
+
—


 


hsa-mir-4677
MI0017308
1
chr1
243346176
243346255
+
✓


 


hsa-mir-4676
MI0017307
—
chr10
72721029
72721100
+
—


 


hsa-mir-4675
MI0017306
—
chr10
20551970
20552046
+
—


 


hsa-mir-4674
MI0017305
8
chr9
136546173
136546259
−
—


 


hsa-mir-4673
MI0017304
—
chr9
136519568
136519626
−
—


 


hsa-mir-4672
MI0017303
46
chr9
127869415
127869495
−
—


 


hsa-mir-4671
MI0017302
—
chr1
234306467
234306539
+
—


 


hsa-mir-4670
MI0017301
—
chr9
92527984
92528058
−
—


 


hsa-mir-4669
MI0017300
2
chr9
134379411
134379472
+
—


 


hsa-mir-4668
MI0017298
—
chr9
111932100
111932169
+
—


 


hsa-mir-4667
MI0017297
3
chr9
35608094
35608159
+
—


 


hsa-mir-4666b
MI0019299
—
chrX
29574278
29574358
+
—


 


hsa-mir-4666a
MI0017296
—
chr1
228462074
228462152
+
—


 


hsa-mir-4665
MI0017295
—
chr9
6007826
6007904
+
—


 


hsa-mir-4664
MI0017294
—
chr8
143733083
143733153
−
—


 


hsa-mir-4663
MI0017292
110
chr8
123215788
123215863
−
—


 


hsa-mir-4662b
MI0017293
1
chr8
124821978
124822058
−
—


 


hsa-mir-4662a
MI0017290
2
chr8
124821985
124822051
+
—


 


hsa-mir-4661
MI0017289
—
chr8
91205485
91205559
+
—


 


hsa-mir-4660
MI0017288
22
chr8
9048445
9048518
+
—


 


hsa-mir-466
MI0014157
13
chr3
31161704
31161787
−
—


 


hsa-mir-4659b
MI0017291
—
chr8
6745168
6745240
−
—


 


hsa-mir-4659a
MI0017287
2
chr8
6745164
6745244
+
—


 


hsa-mir-4658
MI0017286
—
chr7
100156605
100156669
−
—


 


hsa-mir-4657
MI0017285
—
chr7
44881748
44881800
−
—


 


hsa-mir-4656
MI0017284
11
chr7
4788565
4788639
−
—


 


hsa-mir-4655
MI0017283
2
chr7
1844180
1844253
−
—


 


hsa-mir-4654
MI0017282
—
chr1
162157107
162157182
+
—


 


hsa-mir-4653
MI0017281
—
chr7
101159473
101159555
+
—


 


hsa-mir-4652
MI0017280
—
chr7
93716928
93717005
+
—


 


hsa-mir-4651
MI0017279
5
chr7
75915197
75915269
+
—


 


hsa-mir-4650-2
MI0017278
—
chr7
72697903
72697978
+
—


 


hsa-mir-4650-1
MI0017277
—
chr7
67114322
67114397
−
—


 


hsa-mir-4649
MI0017276
—
chr7
44110849
44110912
+
—


 


hsa-mir-4648
MI0017275
—
chr7
2527074
2527145
+
—


 


hsa-mir-4647
MI0017274
5
chr6
44254206
44254285
−
—


 


hsa-mir-4646
MI0017273
—
chr6
31701029
31701091
−
—


 


hsa-mir-4645
MI0017272
—
chr6
2854031
2854107
−
—


 


hsa-mir-4644
MI0017271
5
chr6
170330761
170330844
+
—


 


hsa-mir-4643
MI0017270
—
chr6
91521660
91521737
+
—


 


hsa-mir-4642
MI0017269
—
chr6
44435641
44435722
+
—


 


hsa-mir-4641
MI0017268
33
chr6
41598723
41598788
+
—


 


hsa-mir-4640
MI0017267
3
chr6
30890883
30890972
+
—


 


hsa-mir-4639
MI0017266
—
chr6
16141556
16141624
+
—


 


hsa-mir-4638
MI0017265
1
chr5
181222566
181222633
−
—


 


hsa-mir-4637
MI0017264
—
chr5
14825929
14826012
−
—


 


hsa-mir-4636
MI0017263
—
chr5
9053816
9053895
−
—


 


hsa-mir-4635
MI0017262
2
chr5
1062896
1062974
−
—


 


hsa-mir-4634
MI0017261
—
chr5
174751734
174751787
+
—


 


hsa-mir-4633
MI0017260
—
chr5
129097688
129097766
+
—


 


hsa-mir-4632
MI0017259
—
chr1
12191713
12191773
+
—


 


hsa-mir-455
MI0003513
353
chr9
114209434
114209529
+
—


 


hsa-mir-4540
MI0016911
—
chr9
36864254
36864308
−
—


 


hsa-mir-454
MI0003820
207
chr17
59137758
59137872
−
✓


 


hsa-mir-4539
MI0016910
8
chr14
105857513
105857572
−
—


 


hsa-mir-4538
MI0016909
2
chr14
105858165
105858242
−
—


 


hsa-mir-4537
MI0016908
2
chr14
105859484
105859553
−
—


 


hsa-mir-4536-2
MI0019149
70
chrX
55451495
55451582
+
—


 


hsa-mir-4536-1
MI0016906
—
chrX
55451495
55451582
−
—


 


hsa-mir-4535
MI0016903
—
chr22
48780295
48780353
+
—


 


hsa-mir-4534
MI0016901
—
chr22
37988794
37988853
+
—


 


hsa-mir-4533
MI0016900
22
chr20
60478111
60478181
+
—


 


hsa-mir-4531
MI0016898
42
chr19
44653686
44653732
−
—


 


hsa-mir-4530
MI0016897
—
chr19
39409623
39409678
−
—


 


hsa-mir-4529
MI0016896
—
chr18
55479221
55479298
+
—


 


hsa-mir-4528
MI0016895
—
chr18
53237101
53237190
+
—


 


hsa-mir-4527
MI0016894
—
chr18
47380496
47380565
+
—


 


hsa-mir-4526
MI0016893
—
chr18
13611114
13611200
+
—


 


hsa-mir-4525
MI0016892
4
chr17
82668233
82668307
−
—


 


hsa-mir-4524b
MI0019114
45
chr17
69099542
69099656
+
—


 


hsa-mir-4524a
MI0016891
—
chr17
69099564
69099632
−
✓


 


hsa-mir-4523
MI0016890
—
chr17
29390662
29390730
+
—


 


hsa-mir-4522
MI0016889
—
chr17
27293910
27293996
−
—


 


hsa-mir-4521
MI0016887
654
chr17
8186945
8187004
+
—


 


hsa-mir-4520-2
MI0017358
—
chr17
6655449
6655502
+
—


 


hsa-mir-4520-1
MI0016886
—
chr17
6655440
6655509
−
—


 


hsa-mir-452
MI0001733
123
chrX
151959628
151959712
−
✓


 


hsa-mir-451b
MI0017360
—
chr17
28861371
28861438
+
—


 


hsa-mir-451a
MI0001729
3855
chr17
28861369
28861440
−
—


 


hsa-mir-4519
MI0016885
—
chr16
30875266
30875323
−
—


 


hsa-mir-4518
MI0016884
2
chr16
30503919
30504001
+
—


 


hsa-mir-4517
MI0016883
10
chr16
28958583
28958661
+
—


 


hsa-mir-4516
MI0016882
229
chr16
2133119
2133204
+
—


 


hsa-mir-4515
MI0016881
4
chr15
83067335
83067415
+
—


 


hsa-mir-4514
MI0016880
—
chr15
80997417
80997473
−
—


 


hsa-mir-4513
MI0016879
16
chr15
74788672
74788757
−
—


 


hsa-mir-4512
MI0016878
11
chr15
66496958
66497034
−
—


 


hsa-mir-4511
MI0016877
6
chr15
65719246
65719332
−
—


 


hsa-mir-4510
MI0016876
237
chr15
35926856
35926923
+
—


 


hsa-mir-450b
MI0005531
27
chrX
134540185
134540262
−
✓


 


hsa-mir-450a-2
MI0003187
275
chrX
134540508
134540607
−
✓


 


hsa-mir-450a-1
MI0001652
275
chrX
134540341
134540431
−
✓


 


hsa-mir-4509-3
MI0016875
—
chr15
28490752
28490845
−
—


 


hsa-mir-4509-2
MI0016874
—
chr15
28426491
28426584
+
—


 


hsa-mir-4509-1
MI0016873
—
chr15
23197827
23197920
+
—


 


hsa-mir-4508
MI0016872
31
chr15
23562062
23562131
−
—


 


hsa-mir-4507
MI0016871
—
chr14
105858124
105858175
−
—


 


hsa-mir-4506
MI0016869
—
chr14
93948226
93948302
−
—


 


hsa-mir-4505
MI0016868
—
chr14
73758747
73758819
+
—


 


hsa-mir-4504
MI0016867
—
chr14
50299855
50299946
−
—


 


hsa-mir-4503
MI0016866
119
chr14
36952309
36952391
−
—


 


hsa-mir-4502
MI0016865
—
chr13
114273828
114273908
+
—


 


hsa-mir-4501
MI0016864
—
chr13
96427229
96427292
+
—


 


hsa-mir-4500
MI0016863
106
chr13
87618665
87618740
−
—


 


hsa-mir-449c
MI0003823
9
chr5
55172262
55172353
−
—


 


hsa-mir-449b
MI0003673
120
chr5
55170646
55170742
−
—


 


hsa-mir-449a
MI0001648
102
chr5
55170532
55170622
−
—


 


hsa-mir-4499
MI0016862
—
chr13
20433778
20433846
−
—


 


hsa-mir-4498
MI0016860
46
chr12
120155434
120155499
−
—


 


hsa-mir-4497
MI0016859
96
chr12
109833348
109833436
+
—


 


hsa-mir-4496
MI0016858
—
chr12
108635810
108635870
+
—


 


hsa-mir-4495
MI0016857
—
chr12
97939056
97939121
−
—


 


hsa-mir-4494
MI0016856
—
chr12
47364186
47364269
−
—


 


hsa-mir-4493
MI0016855
23
chr11
123381440
123381512
−
—


 


hsa-mir-4492
MI0016854
12
chr11
118910708
118910787
+
—


 


hsa-mir-4491
MI0016853
—
chr11
111347757
111347824
+
—


 


hsa-mir-4490
MI0016852
—
chr11
90555774
90555857
−
—


 


hsa-mir-4489
MI0016850
—
chr11
65649192
65649253
+
—


 


hsa-mir-4488
MI0016849
67
chr11
61508596
61508657
+
—


 


hsa-mir-4487
MI0016848
—
chr11
47400970
47401042
+
✓


 


hsa-mir-4486
MI0016847
—
chr11
19575310
19575372
+
—


 


hsa-mir-4485
MI0016846
20
chr11
10508270
10508326
−
—


 


hsa-mir-4484
MI0016845
24
chr10
125819740
125819822
+
—


 


hsa-mir-4483
MI0016844
—
chr10
113777993
113778054
−
—


 


hsa-mir-4482
MI0016843
—
chr10
104268336
104268405
−
—


 


hsa-mir-4481
MI0016842
—
chr10
12653138
12653197
−
—


 


hsa-mir-4480
MI0016841
100
chr10
12578753
12578823
+
—


 


hsa-mir-448
MI0001637
—
chrX
114823454
114823564
+
—


 


hsa-mir-4479
MI0016838
—
chr9
136886733
136886803
+
—


 


hsa-mir-4478
MI0016831
—
chr9
122120082
122120167
−
—


 


hsa-mir-4477b
MI0016830
11
chr9
41233755
41233835
−
—


 


hsa-mir-4477a
MI0016829
3
chr9
41233755
41233835
+
—


 


hsa-mir-4476
MI0016828
—
chr9
36893462
36893531
−
—


 


hsa-mir-4475
MI0016827
—
chr9
36823539
36823599
−
—


 


hsa-mir-4474
MI0016826
—
chr9
20502265
20502342
−
—


 


hsa-mir-4473
MI0016825
—
chr9
20411148
20411238
−
—


 


hsa-mir-4472-2
MI0016824
65
chr12
116428252
116428318
−
—


 


hsa-mir-4472-1
MI0016823
24
chr8
142176339
142176418
+
—


 


hsa-mir-4471
MI0016822
—
chr8
100382763
100382845
+
—


 


hsa-mir-4470
MI0016821
—
chr8
61714788
61714859
+
—


 


hsa-mir-4469
MI0016820
—
chr8
42896197
42896275
−
—


 


hsa-mir-4468
MI0016819
—
chr7
138123758
138123821
+
—


 


hsa-mir-4467
MI0016818
—
chr7
102471469
102471531
+
—


 


hsa-mir-4466
MI0016817
1
chr6
156779678
156779731
−
—


 


hsa-mir-4465
MI0016816
—
chr6
140683814
140683883
+
—


 


hsa-mir-4464
MI0016812
—
chr6
90312742
90312833
+
—


 


hsa-mir-4463
MI0016811
—
chr6
75428407
75428473
+
—


 


hsa-mir-4462
MI0016810
—
chr6
37555365
37555422
−
—


 


hsa-mir-4460
MI0016806
—
chr5
129397062
129397147
−
—


 


hsa-mir-4458
MI0016804
—
chr5
8460925
8460999
+
—


 


hsa-mir-4457
MI0016803
—
chr5
1309310
1309377
−
—


 


hsa-mir-4456
MI0016802
—




—


 


hsa-mir-4455
MI0016801
30
chr4
184938383
184938440
−
—


 


hsa-mir-4454
MI0016800
457
chr4
163093574
163093628
−
—


 


hsa-mir-4453
MI0016799
—
chr4
152536428
152536516
+
—


 


hsa-mir-4452
MI0016798
7
chr4
86542482
86542552
−
—


 


hsa-mir-4451
MI0016797
13
chr4
85722468
85722533
+
—


 


hsa-mir-4450
MI0016795
—
chr4
76573568
76573632
+
—


 


hsa-mir-4449
MI0016792
943
chr4
52712682
52712747
+
—


 


hsa-mir-4448
MI0016791
59
chr3
183886800
183886885
+
—


 


hsa-mir-4447
MI0016790
—
chr3
116850277
116850367
−
—


 


hsa-mir-4446
MI0016789
1
chr3
113594876
113594942
+
—


 


hsa-mir-4445
MI0016788
100
chr3
109602828
109602897
+
—


 


hsa-mir-4444-2
MI0019111
9
chr3
75214476
75214549
+
—


 


hsa-mir-4444-1
MI0016787
9
chr2
177212726
177212799
+
—


 


hsa-mir-4443
MI0016786
872
chr3
48196564
48196616
+
—


 


hsa-mir-4442
MI0016785
6
chr3
25664873
25664939
−
—


 


hsa-mir-4441
MI0016784
—
chr2
239085827
239085926
−
—


 


hsa-mir-4440
MI0016783
—
chr2
239068817
239068914
−
—


 


hsa-mir-4439
MI0016782
—
chr2
225010461
225010540
−
—


 


hsa-mir-4438
MI0016781
138
chr2
213758067
213758159
+
—


 


hsa-mir-4437
MI0016778
—
chr2
181305593
181305652
−
—


 


hsa-mir-4436b-2
MI0019110
7
chr2
110284853
110284943
+
—


 


hsa-mir-4436b-1
MI0017425
7
chr2
110086433
110086523
−
—


 


hsa-mir-4436a
MI0016776
—
chr2
88812370
88812454
+
—


 


hsa-mir-4435-2
MI0016777
—
chr2
111321013
111321086
−
—


 


hsa-mir-4435-1
MI0016775
—
chr2
87629755
87629834
+
—


 


hsa-mir-4434
MI0016774
—
chr2
64525513
64525565
+
—


 


hsa-mir-4433b
MI0025511
—
chr2
64340747
64340848
−
—


 


hsa-mir-4433a
MI0016773
5
chr2
64340759
64340839
+
—


 


hsa-mir-4432
MI0016772
16
chr2
60387362
60387445
−
—


 


hsa-mir-4431
MI0016771
5
chr2
52702522
52702615
−
—


 


hsa-mir-4430
MI0016769
12
chr2
33418516
33418564
+
—


 


hsa-mir-4429
MI0016768
1989
chr2
11540605
11540677
−
—


 


hsa-mir-4428
MI0016767
—
chr1
237471119
237471191
+
—


 


hsa-mir-4427
MI0016766
—
chr1
233624152
233624219
+
—


 


hsa-mir-4426
MI0016765
—
chr1
192716328
192716390
+
—


 


hsa-mir-4425
MI0016764
14
chr1
25023503
25023586
+
—


 


hsa-mir-4424
MI0016763
—
chr1
178677749
178677834
+
—


 


hsa-mir-4423
MI0016760
10
chr1
85133794
85133873
+
✓


 


hsa-mir-4422
MI0016759
—
chr1
55225641
55225723
+
—


 


hsa-mir-4421
MI0016758
—
chr1
51059837
51059905
+
—


 


hsa-mir-4420
MI0016757
—
chr1
30739156
30739232
−
—


 


hsa-mir-4418
MI0016754
44
chr1
22266239
22266300
+
—


 


hsa-mir-4330
MI0015902
11
chrX
151168222
151168326
+
—


 


hsa-mir-433
MI0001723
2
chr14
100881886
100881978
+
✓


 


hsa-mir-4329
MI0015901
4
chrX
112780718
112780788
−
—


 


hsa-mir-4328
MI0015904
80
chrX
78901194
78901249
−
—


 


hsa-mir-4327
MI0015867
57
chr21
30375294
30375378
−
—


 


hsa-mir-4326
MI0015866
—
chr20
63286808
63286866
+
—


 


hsa-mir-4325
MI0015865
—
chr20
57321502
57321591
−
—


 


hsa-mir-4324
MI0015854
56
chr19
49308797
49308868
−
—


 


hsa-mir-4323
MI0015853
—
chr19
42133445
42133513
−
—


 


hsa-mir-4322
MI0015851
9
chr19
10230413
10230485
+
—


 


hsa-mir-4321
MI0015852
—
chr19
2250639
2250718
+
—


 


hsa-mir-4320
MI0015849
—
chr18
50126499
50126563
−
—


 


hsa-mir-432
MI0003133
55
chr14
100884483
100884576
+
—


 


hsa-mir-4319
MI0015848
—
chr18
44970082
44970166
−
—


 


hsa-mir-4318
MI0015847
62
chr18
37657135
37657215
+
—


 


hsa-mir-4317
MI0015850
1
chr18
6374361
6374425
−
—


 


hsa-mir-4316
MI0015845
209
chr17
77396984
77397054
−
—


 


hsa-mir-4315-2
MI0015983
—
chr17
64822030
64822102
−
—


 


hsa-mir-4315-1
MI0015844
—
chr17
45475363
45475435
−
—


 


hsa-mir-4314
MI0015846
150
chr17
8088056
8088147
+
—


 


hsa-mir-4313
MI0015843
28
chr15
75762215
75762315
−
—


 


hsa-mir-4312
MI0015842
57
chr15
68801850
68801925
−
—


 


hsa-mir-4311
MI0015841
56
chr15
66040233
66040332
+
—


 


hsa-mir-4310
MI0015840
77
chr15
41866495
41866551
−
—


 


hsa-mir-431
MI0001721
—
chr14
100881007
100881120
+
—


 


hsa-mir-4309
MI0015837
—
chr14
102539644
102539726
+
—


 


hsa-mir-4308
MI0015839
6
chr14
54878113
54878193
−
—


 


hsa-mir-4307
MI0015838
—
chr14
26908642
26908725
+
—


 


hsa-mir-4306
MI0015836
135
chr13
99643059
99643149
+
—


 


hsa-mir-4305
MI0015835
214
chr13
39664034
39664135
−
—


 


hsa-mir-4304
MI0015832
13
chr12
123010667
123010728
−
—


 


hsa-mir-4303
MI0015834
—
chr12
97995383
97995448
−
—


 


hsa-mir-4302
MI0015833
—
chr12
25874020
25874079
−
—


 


hsa-mir-4301
MI0015828
8
chr11
113450023
113450088
−
—


 


hsa-mir-4300
MI0015831
—
chr11
81890741
81890836
−
—


 


hsa-mir-4299
MI0015829
—
chr11
11656651
11656722
−
—


 


hsa-mir-4298
MI0015830
9
chr11
1859464
1859536
−
—


 


hsa-mir-4297
MI0015824
—
chr10
129843299
129843374
−
—


 


hsa-mir-4296
MI0015823
6
chr10
125032783
125032870
−
—


 


hsa-mir-4295
MI0015822
—
chr10
112634170
112634254
+
—


 


hsa-mir-4294
MI0015827
171
chr10
48985512
48985587
−
—


 


hsa-mir-4293
MI0015826
—
chr10
14383200
14383277
−
—


 


hsa-mir-4292
MI0015897
—
chr9
136830957
136831023
+
—


 


hsa-mir-4291
MI0015900
—
chr9
93819357
93819421
+
—


 


hsa-mir-4290
MI0015899
145
chr9
90023441
90023535
−
—


 


hsa-mir-429
MI0001641
381
chr1
1169005
1169087
+
—


 


hsa-mir-4289
MI0015898
852
chr9
88745836
88745905
−
—


 


hsa-mir-4288
MI0015896
8
chr8
28505116
28505182
−
—


 


hsa-mir-4287
MI0015895
—
chr8
27886039
27886116
−
—


 


hsa-mir-4286
MI0015894
310
chr8
10666978
10667070
+
—


 


hsa-mir-4285
MI0015891
7
chr7
102293103
102293187
+
—


 


hsa-mir-4284
MI0015893
22
chr7
73711317
73711397
+
—


 


hsa-mir-4283-2
MI0015982
—
chr7
63621090
63621169
+
—


 


hsa-mir-4283-1
MI0015892
—
chr7
56955785
56955864
−
—


 


hsa-mir-4282
MI0015890
—
chr6
72967687
72967753
−
—


 


hsa-mir-4281
MI0015885
11
chr5
176629439
176629500
−
—


 


hsa-mir-4280
MI0015889
—
chr5
87114879
87114954
−
—


 


hsa-mir-4279
MI0015887
13
chr5
31936102
31936159
−
—


 


hsa-mir-4278
MI0015888
—
chr5
6827853
6827921
−
—


 


hsa-mir-4277
MI0015886
—
chr5
1708785
1708868
−
—


 


hsa-mir-4276
MI0015882
—
chr4
174423795
174423864
+
—


 


hsa-mir-4275
MI0015883
266
chr4
28819582
28819668
+
—


 


hsa-mir-4274
MI0015884
33
chr4
7460028
7460118
+
—


 


hsa-mir-4273
MI0015881
—
chr3
75738280
75738363
+
—


 


hsa-mir-4272
MI0015880
—
chr3
67225464
67225527
+
—


 


hsa-mir-4271
MI0015879
24
chr3
49274120
49274186
+
—


 


hsa-mir-4270
MI0015878
14
chr3
15496239
15496308
−
—


 


hsa-mir-4269
MI0015875
—
chr2
239305462
239305545
+
—


 


hsa-mir-4268
MI0015874
—
chr2
219906502
219906565
−
—


 


hsa-mir-4267
MI0015871
—
chr2
110069961
110070042
−
—


 


hsa-mir-4266
MI0015870
—
chr2
109313571
109313625
−
—


 


hsa-mir-4265
MI0015869
5
chr2
109141490
109141588
−
—


 


hsa-mir-4264
MI0015877
877
chr2
79649294
79649359
−
—


 


hsa-mir-4263
MI0015876
—
chr2
27996367
27996449
+
—


 


hsa-mir-4262
MI0015872
—
chr2
11836933
11836986
−
—


 


hsa-mir-4261
MI0015868
—
chr2
10192614
10192671
−
—


 


hsa-mir-4260
MI0015859
350
chr1
209623444
209623510
−
—


 


hsa-mir-4259
MI0015858
10
chr1
159899979
159900079
−
—


 


hsa-mir-4258
MI0015857
9
chr1
154975693
154975783
+
—


 


hsa-mir-4257
MI0015856
12
chr1
150551929
150552014
+
—


 


hsa-mir-4256
MI0015855
—
chr1
112461770
112461833
−
—


 


hsa-mir-4255
MI0015863
—
chr1
37161563
37161634
+
—


 


hsa-mir-4254
MI0015862
—
chr1
31758660
31758735
−
—


 


hsa-mir-4253
MI0015860
—
chr1
22863159
22863226
−
—


 


hsa-mir-4252
MI0015864
—
chr1
6429834
6429896
−
—


 


hsa-mir-4251
MI0015861
—
chr1
3127975
3128035
+
—


 


hsa-mir-425
MI0001448
1131
chr3
49020148
49020234
−
✓


 


hsa-mir-424
MI0001446
3666
chrX
134546614
134546711
−
✓


 


hsa-mir-423
MI0001445
3706
chr17
30117079
30117172
+
✓


 


hsa-mir-422a
MI0001444
7
chr15
63870930
63871019
−
—


 


hsa-mir-421
MI0003685
120
chrX
74218377
74218461
−
✓


 


hsa-mir-412
MI0002464
—
chr14
101065447
101065537
+
—


 


hsa-mir-411
MI0003675
86
chr14
101023325
101023420
+
✓


 


hsa-mir-410
MI0002465
12
chr14
101065912
101065991
+
✓


 


hsa-mir-409
MI0001735
3
chr14
101065300
101065378
+
✓


 


hsa-mir-3978
MI0016996
—
chrX
110082118
110082218
+
—


 


hsa-mir-3977
MI0016995
—
chr5
82840155
82840224
+
—


 


hsa-mir-3976
MI0016994
—
chr18
5840695
5840833
+
—


 


hsa-mir-3975
MI0016993
100
chr18
35591737
35591806
+
—


 


hsa-mir-3974
MI0016992
—
chr12
17673299
17673394
+
—


 


hsa-mir-3973
MI0016991
200
chr11
36010098
36010204
+
—


 


hsa-mir-3972
MI0016990
122
chr1
17277889
17277975
+
—


 


hsa-mir-3960
MI0016964
95
chr9
127785833
127785923
+
—


 


hsa-mir-3945
MI0016602
—
chr4
184851013
184851110
−
—


 


hsa-mir-3944
MI0016601
10
chr10
133371556
133371663
−
✓


 


hsa-mir-3943
MI0016600
—
chr7
43150895
43150994
+
—


 


hsa-mir-3942
MI0016599
—
chr15
35372256
35372364
−
✓


 


hsa-mir-3941
MI0016598
—
chr10
122416965
122417067
+
—


 


hsa-mir-3940
MI0016597
—
chr19
6416410
6416511
−
—


 


hsa-mir-3939
MI0016596
—
chr6
166997807
166997912
−
—


 


hsa-mir-3938
MI0016594
—
chr3
55852492
55852594
−
—


 


hsa-mir-3937
MI0016593
—
chrX
39661216
39661321
+
—


 


hsa-mir-3936
MI0016592
—
chr5
132365490
132365599
−
—


 


hsa-mir-3935
MI0016591
3
chr16
56245520
56245623
+
—


 


hsa-mir-3934
MI0016590
1
chr6
33698128
33698234
+
—


 


hsa-mir-3929
MI0016439
238
chr18
35934088
35934142
−
—


 


hsa-mir-3928
MI0016438
1
chr22
31160062
31160119
−
—


 


hsa-mir-3927
MI0016435
—
chr9
109511475
109511545
−
—


 


hsa-mir-3926-2
MI0016437
6
chr8
12727237
12727299
+
—


 


hsa-mir-3926-1
MI0016434
5
chr8
12727232
12727304
−
—


 


hsa-mir-3925
MI0016433
—
chr6
36622436
36622512
−
—


 


hsa-mir-3924
MI0016432
100
chr10
57304479
57304559
−
—


 


hsa-mir-3923
MI0016430
—
chr3
79507887
79507969
+
—


 


hsa-mir-3922
MI0016429
—
chr12
104591633
104591716
+
—


 


hsa-mir-3921
MI0016428
4
chr3
99964314
99964398
−
—


 


hsa-mir-3920
MI0016427
—
chr11
101519820
101519905
−
—


 


hsa-mir-3919
MI0016425
—
chr3
159282646
159282734
+
—


 


hsa-mir-3918
MI0016424
—
chr6
158764661
158764753
−
—


 


hsa-mir-3917
MI0016423
3
chr1
25906362
25906454
−
—


 


hsa-mir-3916
MI0016422
14
chr1
247201967
247202060
−
—


 


hsa-mir-3915
MI0016420
—
chrX
32583656
32583752
−
—


 


hsa-mir-3914-2
MI0016421
—
chr7
71307674
71307768
+
—


 


hsa-mir-3914-1
MI0016419
—
chr7
71307672
71307770
−
—


 


hsa-mir-3913-2
MI0016418
4
chr12
69584723
69584822
+
—


 


hsa-mir-3913-1
MI0016417
2
chr12
69584722
69584823
−
—


 


hsa-mir-3912
MI0016416
—
chr5
171386656
171386760
−
—


 


hsa-mir-3911
MI0016415
1
chr9
127690687
127690795
−
—


 


hsa-mir-3910-2
MI0016431
2
chr9
91636264
91636345
−
—


 


hsa-mir-3910-1
MI0016414
2
chr9
91636251
91636361
+
—


 


hsa-mir-3909
MI0016413
—
chr22
35335640
35335758
+
✓


 


hsa-mir-3908
MI0016412
72
chr12
123536409
123536534
+
—


 


hsa-mir-3907
MI0016410
36
chr7
151433489
151433639
−
—


 


hsa-mir-384
MI0001145
—
chrX
76919273
76919360
−
—


 


hsa-mir-383
MI0000791
8
chr8
14853438
14853510
−
—


 


hsa-mir-382
MI0000790
23
chr14
101054306
101054381
+
✓


 


hsa-mir-381
MI0000789
22
chr14
101045920
101045994
+
✓


 


hsa-mir-380
MI0000788
1
chr14
101025017
101025077
+
✓


 


hsa-mir-379
MI0000787
263
chr14
101022066
101022132
+
✓


 


hsa-mir-378j
MI0021273
7
chr17
37614931
37615039
−
—


 


hsa-mir-3781
MI0016902
5084
chr22
41923222
41923297
−
—


 


hsa-mir-378h
MI0016808
3791
chr5
154829458
154829540
+
—


 


hsa-mir-378g
MI0016761
4642




—


 


hsa-mir-378f
MI0016756
4762
chr1
23929070
23929147
+
—


 


hsa-mir-378e
MI0016750
3130
chr5
170028488
170028566
+
—


 


hsa-mir-378d-2
MI0003840
4789
chr8
93916022
93916119
−
—


 


hsa-mir-378d-1
MI0016749
3415
chr4
5923275
5923328
−
—


 


hsa-mir-378c
MI0015825
4978
chr10
130962588
130962668
−
—


 


hsa-mir-378b
MI0014154
3955
chr3
10330229
10330285
+
—


 


hsa-mir-378a
MI0000786
6009
chr5
149732825
149732890
+
✓


 


hsa-mir-377
MI0000785
15
chr14
101062050
101062118
+
✓


 


hsa-mir-376c
MI0000776
214
chr14
101039690
101039755
+
✓


 


hsa-mir-376b
MI0002466
115
chr14
101040436
101040535
+
✓


 


hsa-mir-376a-2
MI0003529
119
chr14
101040069
101040148
+
✓


 


hsa-mir-376a-1
MI0000784
120
chr14
101040782
101040849
+
✓


 


hsa-mir-375
MI0000783
3418
chr2
219001645
219001708
−
✓


 


hsa-mir-374c
MI0016684
8
chrX
74218549
74218618
+
—


 


hsa-mir-374b
MI0005566
1108
chrX
74218547
74218618
−
✓


 


hsa-mir-374a
MI0000782
1271
chrX
74287286
74287357
−
✓


 


hsa-mir-373
MI0000781
2
chr19
53788705
53788773
+
—


 


hsa-mir-372
MI0000780
91
chr19
53787890
53787956
+
—


 


hsa-mir-371b
MI0017393
23
chr19
53787677
53787742
−
—


 


hsa-mir-371a
MI0000779
—
chr19
53787675
53787741
+
—


 


hsa-mir-3714
MI0016135
5
chr3
16933196
16933260
+
—


 


hsa-mir-3713
MI0016134
—




—


 


hsa-mir-370
MI0000778
9
chr14
100911139
100911213
+
✓


 


hsa-mir-3692
MI0016093
—
chr6
157529132
157529200
+
—


 


hsa-mir-3691
MI0016092
—
chr6
5148233
5148322
−
—


 


hsa-mir-3690-2
MI0023561
—
chrY
1293918
1293992
+
—


 


hsa-mir-3690-1
MI0016091
—
chrX
1293918
1293992
+
—


 


hsa-mir-369
MI0000777
53
chr14
101065598
101065667
+
✓


 


hsa-mir-3689f
MI0016837
25
chr9
134850742
134850807
−
—


 


hsa-mir-3689e
MI0016836
—
chr9
134850570
134850641
−
—


 


hsa-mir-3689d-2
MI0016835
—
chr9
134850277
134850356
−
—


 


hsa-mir-3689d-1
MI0016834
—
chr9
134849609
134849682
−
—


 


hsa-mir-3689c
MI0016832
—
chr9
134849298
134849369
−
—


 


hsa-mir-3689b
MI0016411
—
chr9
134850125
134850272
−
—


 


hsa-mir-3689a
MI0016090
—
chr9
134849487
134849564
−
—


 


hsa-mir-3688-2
MI0017447
3
chr4
159128805
159128891
+
—


 


hsa-mir-3688-1
MI0016089
73
chr4
159128802
159128894
−
—


 


hsa-mir-3686
MI0016087
—
chr8
129484057
129484142
−
—


 


hsa-mir-3685
MI0016086
—
chr12
95309923
95309984
+
—


 


hsa-mir-3684
MI0016085
—
chr4
98997387
98997460
+
—


 


hsa-mir-3683
MI0016084
—
chr7
7066964
7067045
−
—


 


hsa-mir-3682
MI0016083
4
chr2
53849122
53849205
−
—


 


hsa-mir-3681
MI0016082
—
chr2
12199130
12199201
+
—


 


hsa-mir-3680-2
MI0019113
3
chr16
29599179
29599265
−
—


 


hsa-mir-3680-1
MI0016081
3
chr16
21506049
21506135
−
—


 


hsa-mir-3679
MI0016080
—
chr2
134127125
134127192
+
—


 


hsa-mir-3678
MI0016079
10
chr17
75406069
75406162
+
—


 


hsa-mir-3677
MI0016078
8
chr16
2270713
2270772
+
✓


 


hsa-mir-3675
MI0016076
—
chr1
16858949
16859021
−
—


 


hsa-mir-3674
MI0016075
—
chr8
1801125
1801192
+
—


 


hsa-mir-3672
MI0016073
—
chrX
121370972
121371053
+
—


 


hsa-mir-3671
MI0016072
—
chr1
65057755
65057842
−
—


 


hsa-mir-3670-4
MI0031514
—
chr16
18488301
18488365
−
—


 


hsa-mir-3670-3
MI0031513
—
chr16
18405698
18405762
−
—


 


hsa-mir-3670-2
MI0019112
—
chr16
16306370
16306434
+
—


 


hsa-mir-3670-1
MI0016071
—
chr16
14907717
14907781
+
—


 


hsa-mir-367
MI0000775
9
chr4
112647874
112647941
−
—


 


hsa-mir-3668
MI0016069
—
chr6
140205252
140205326
+
—


 


hsa-mir-3667
MI0016068
—
chr22
49543393
49543466
−
—


 


hsa-mir-3666
MI0016067
—
chr7
114653345
114653455
+
—


 


hsa-mir-3665
MI0016066
63
chr13
77698012
77698116
−
—


 


hsa-mir-3664
MI0016065
—
chr11
70872270
70872368
−
—


 


hsa-mir-3663
MI0016064
38
chr10
117167678
117167774
−
—


 


hsa-mir-3662
MI0016063
—
chr6
134979338
134979432
−
—


 


hsa-mir-3661
MI0016062
5
chr5
134225757
134225852
+
—


 


hsa-mir-3660
MI0016061
29
chr5
90016621
90016720
−
—


 


hsa-mir-365b
MI0000769
1025
chr17
31575411
31575521
+
✓


 


hsa-mir-365a
MI0000767
1023
chr16
14309285
14309371
+
✓


 


hsa-mir-3659
MI0016060
—
chr1
38089231
38089329
+
—


 


hsa-mir-3658
MI0016058
3
chr1
165907921
165907976
+
—


 


hsa-mir-3657
MI0016057
—
chr12
112037599
112037715
−
✓


 


hsa-mir-3655
MI0016055
—
chr5
140647844
140647926
+
—


 


hsa-mir-3654
MI0016054
—
chr7
133034860
133034915
−
—


 


hsa-mir-3652
MI0016052
5
chr12
103930425
103930555
+
—


 


hsa-mir-3651
MI0016051
3
chr9
92292458
92292547
−
—


 


hsa-mir-3650
MI0016050
—
chr5
38557502
38557561
−
—


 


hsa-mir-3649
MI0016049
28
chr12
1660315
1660380
−
—


 


hsa-mir-3648-2
MI0031512
8
chr21
8986999
8987178
+
—


 


hsa-mir-3648-1
MI0016048
8
chr21
8208473
8208652
+
—


 


hsa-mir-3646
MI0016046
18
chr20
44408120
44408203
+
—


 


hsa-mir-363
MI0000764
757
chrX
134169378
134169452
−
✓


 


hsa-mir-3622b
MI0016014
6
chr8
27701673
27701767
−
—


 


hsa-mir-3622a
MI0016013
5
chr8
27701677
27701759
+
—


 


hsa-mir-3621
MI0016012
—
chr9
137169186
137169270
−
—


 


hsa-mir-3620
MI0016011
—
chr1
228097263
228097341
+
—


 


hsa-mir-362
MI0000762
61
chrX
50008964
50009028
+
✓


 


hsa-mir-3619
MI0016009
2
chr22
46091044
46091126
+
—


 


hsa-mir-3618
MI0016008
8
chr22
20085746
20085833
+
—


 


hsa-mir-3617
MI0016007
—
chr20
45705102
45705180
−
—


 


hsa-mir-3616
MI0016006
2
chr20
47166967
47167058
+
✓


 


hsa-mir-3615
MI0016005
11
chr17
74748613
74748699
+
—


 


hsa-mir-3614
MI0016004
19
chr17
56891270
56891355
−
✓


 


hsa-mir-3613
MI0016003
321
chr13
49996415
49996501
−
✓


 


hsa-mir-3612
MI0016002
—
chr12
128294092
128294178
+
—


 


hsa-mir-3611
MI0016001
4
chr10
35079598
35079680
−
✓


 


hsa-mir-3610
MI0016000
13
chr8
116874728
116874800
−
—


 


hsa-mir-361
MI0000760
866
chrX
85903636
85903707
−
✓


 


hsa-mir-3609
MI0015999
73
chr7
98881650
98881729
+
—


 


hsa-mir-3606
MI0015996
9
chr2
188995630
188995692
+
—


 


hsa-mir-3605
MI0015995
1
chr1
33332393
33332492
−
—


 


hsa-mir-3529
MI0017351
—
chr15
88611847
88611924
−
—


 


hsa-mir-34c
MI0000743
379
chr11
111513439
111513515
+
✓


 


hsa-mir-34b
MI0000742
43
chr11
111512938
111513021
+
✓


 


hsa-mir-34a
MI0000268
269
chr1
9151668
9151777
−
✓


 


hsa-mir-346
MI0000826
3
chr10
86264694
86264788
−
—


 


hsa-mir-345
MI0000825
123
chr14
100307859
100307956
+
✓


 


hsa-mir-342
MI0000805
776
chr14
100109655
100109753
+
✓


 


hsa-mir-340
MI0000802
913
chr5
180015303
180015397
−
✓


 


hsa-mir-33b
MI0003646
27
chr17
17813836
17813931
−
✓


 


hsa-mir-33a
MI0000091
256
chr22
41900944
41901012
+
✓


 


hsa-mir-339
MI0000815
238
chr7
1022933
1023026
−
✓


 


hsa-mir-338
MI0000814
97
chr17
81125883
81125949
−
✓


 


hsa-mir-337
MI0000806
21
chr14
100874493
100874585
+
✓


 


hsa-mir-335
MI0000816
3777
chr7
130496111
130496204
+
✓


 


hsa-mir-331
MI0000812
226
chr12
95308420
95308513
+
—


 


hsa-mir-330
MI0000803
70
chr19
45638994
45639087
−
✓


 


hsa-mir-329-2
MI0001726
—
chr14
101027100
101027183
+
✓


 


hsa-mir-329-1
MI0001725
—
chr14
101026785
101026864
+
✓


 


hsa-mir-328
MI0000804
31
chr16
67202321
67202395
−
—


 


hsa-mir-326
MI0000808
14
chr11
75335092
75335186
−
—


 


hsa-mir-325
MI0000824
—
chrX
77005404
77005501
−
—


 


hsa-mir-324
MI0000813
210
chr17
7223297
7223379
−
✓


 


hsa-mir-323b
MI0014206
—
chr14
101056219
101056300
+
—


 


hsa-mir-323a
MI0000807
1
chr14
101025732
101025817
+
✓


 


hsa-mir-320e
MI0014234
4717
chr19
46709282
46709354
−
—


 


hsa-mir-320d-2
MI0008192
2985
chrX
140926160
140926231
−
—


 


hsa-mir-320d-1
MI0008190
4526
chr13
40727816
40727887
−
—


 


hsa-mir-320c-2
MI0008191
4285
chr18
24321675
24321746
+
—


 


hsa-mir-320c-1
MI0003778
3570
chr18
21683518
21683589
+
—


 


hsa-mir-320b-2
MI0003839
5249
chr1
224257040
224257110
−
—


 


hsa-mir-320b-1
MI0003776
5177
chr1
116671746
116671817
+
—


 


hsa-mir-320a
MI0000542
5331
chr8
22244966
22245037
−
✓


 


hsa-mir-3202-2
MI0014253
3
chrX
153981098
153981176
−
—


 


hsa-mir-3202-1
MI0014252
1
chrX
153981097
153981177
+
—


 


hsa-mir-3201
MI0014250
—
chr22
48274364
48274415
+
—


 


hsa-mir-3200
MI0014249
3
chr22
30731557
30731641
+
✓


 


hsa-mir-32
MI0000090
222
chr9
109046229
109046298
−
✓


 


hsa-mir-3199-2
MI0014248
—
chr22
27920526
27920611
+
—


 


hsa-mir-3199-1
MI0014247
—
chr22
27920525
27920612
−
—


 


hsa-mir-3198-2
MI0017335
—
chr12
54231397
54231476
−
—


 


hsa-mir-3198-1
MI0014246
—
chr22
17764180
17764259
−
—


 


hsa-mir-3197
MI0014245
—
chr21
41167557
41167629
+
—


 


hsa-mir-3196
MI0014241
12
chr20
63238779
63238842
+
—


 


hsa-mir-3195
MI0014240
27
chr20
62064802
62064885
+
—


 


hsa-mir-3194
MI0014239
—
chr20
51452905
51452977
−
—


 


hsa-mir-3193
MI0014238
—
chr20
31607186
31607240
+
—


 


hsa-mir-3192
MI0014237
457
chr20
18470615
18470691
+
—


 


hsa-mir-3191
MI0014236
—
chr19
47226944
47227019
−
—


 


hsa-mir-3190
MI0014235
12
chr19
47226942
47227021
+
—


 


hsa-mir-3189
MI0014233
2
chr19
18386562
18386634
+
—


 


hsa-mir-3188
MI0014232
1
chr19
18282077
18282161
+
—


 


hsa-mir-3187
MI0014231
2
chr19
813584
813653
+
—


 


hsa-mir-3186
MI0014229
—
chr17
81451104
81451188
−
—


 


hsa-mir-3185
MI0014227
6
chr17
48724408
48724475
−
—


 


hsa-mir-3184
MI0014226
—
chr17
30117086
30117160
−
—


 


hsa-mir-3183
MI0014225
—
chr17
1022476
1022559
−
—


 


hsa-mir-3182
MI0014224
37
chr16
83508346
83508408
+
—


 


hsa-mir-3181
MI0014223
10
chr16
50742305
50742377
+
—


 


hsa-mir-3180-5
MI0016409
15
chr16
2135977
2136129
−
—


 


hsa-mir-3180-4
MI0016408
14
chr16
15154850
15155002
−
—


 


hsa-mir-3180-3
MI0014217
13
chr16
18402178
18402271
−
✓


 


hsa-mir-3180-2
MI0014215
13
chr16
16309879
16309966
+
✓


 


hsa-mir-3180-1
MI0014214
13
chr16
14911220
14911313
+
✓


 


hsa-mir-3179-4
MI0031510
—
chr16
18494493
18494576
−
—


 


hsa-mir-3179-3
MI0014221
—
chr16
18411894
18411977
−
—


 


hsa-mir-3179-2
MI0014216
—
chr16
16300159
16300242
+
—


 


hsa-mir-3179-1
MI0014213
—
chr16
14901508
14901591
+
—


 


hsa-mir-3178
MI0014212
37
chr16
2531922
2532005
−
—


 


hsa-mir-3177
MI0014211
—
chr16
1734985
1735066
+
—


 


hsa-mir-3176
MI0014210
51
chr16
543277
543366
+
—


 


hsa-mir-3175
MI0014209
1
chr15
92904399
92904475
+
—


 


hsa-mir-3174
MI0014208
—
chr15
90006755
90006841
+
—


 


hsa-mir-3173
MI0014204
7
chr14
95137919
95137986
−
✓


 


hsa-mir-3171
MI0014202
—
chr14
27633205
27633278
−
✓


 


hsa-mir-3170
MI0014201
—
chr13
98208524
98208600
+
—


 


hsa-mir-3169
MI0014200
—
chr13
61199798
61199880
−
—


 


hsa-mir-3168
MI0014199
98
chr13
41101019
41101100
−
—


 


hsa-mir-3167
MI0014198
—
chr11
126988458
126988542
−
—


 


hsa-mir-3166
MI0014196
—
chr11
88176502
88176593
+
—


 


hsa-mir-3165
MI0014195
1
chr11
72072228
72072302
−
—


 


hsa-mir-3164
MI0014194
—
chr11
69083176
69083258
+
—


 


hsa-mir-3163
MI0014193
4
chr11
66934434
66934506
−
—


 


hsa-mir-3162
MI0014192
1
chr11
59595077
59595158
−
—


 


hsa-mir-3161
MI0014191
—
chr11
48096782
48096858
+
—


 


hsa-mir-3160-2
MI0014190
1
chr11
46451807
46451887
+
—


 


hsa-mir-3160-1
MI0014189
—
chr11
46451805
46451889
−
—


 


hsa-mir-3159
MI0014188
638
chr11
18387787
18387860
+
—


 


hsa-mir-3158-2
MI0014187
2
chr10
101601417
101601497
−
✓


 


hsa-mir-3158-1
MI0014186
2
chr10
101601417
101601497
+
✓


 


hsa-mir-3157
MI0014185
—
chr10
96064315
96064399
−
✓


 


hsa-mir-3156-3
MI0014242
—
chr21
13406384
13406460
−
—


 


hsa-mir-3156-2
MI0014230
—
chr18
14830166
14830242
+
—


 


hsa-mir-3156-1
MI0014184
—
chr10
45164014
45164088
+
—


 


hsa-mir-3155b
MI0016839
22
chr10
6152207
6152262
−
—


 


hsa-mir-3155a
MI0014183
2
chr10
6152196
6152277
+
✓


 


hsa-mir-3154
MI0014182
1
chr9
128244947
128245030
−
—


 


hsa-mir-3153
MI0014180
—
chr9
89312225
89312306
+
—


 


hsa-mir-3152
MI0014179
19
chr9
18573306
18573379
+
—


 


hsa-mir-3151
MI0014178
—
chr8
103154614
103154689
+
—


 


hsa-mir-3150b
MI0016426
5
chr8
95072911
95072996
−
—


 


hsa-mir-3150a
MI0014177
24
chr8
95072914
95072993
+
—


 


hsa-mir-3149
MI0014176
130
chr8
76966768
76966850
−
—


 


hsa-mir-3148
MI0014175
—
chr8
29957272
29957348
−
—


 


hsa-mir-3147
MI0014173
—
chr7
57405025
57405090
+
—


 


hsa-mir-3146
MI0014172
—
chr7
19705358
19705436
−
—


 


hsa-mir-3145
MI0014170
—
chr6
138435213
138435294
−
—


 


hsa-mir-3144
MI0014169
3
chr6
120015179
120015257
+
✓


 


hsa-mir-3143
MI0014167
172
chr6
27147626
27147688
+
—


 


hsa-mir-3142
MI0014166
—
chr5
160474402
160474483
+
—


 


hsa-mir-3141
MI0014165
1
chr5
154596012
154596072
−
—


 


hsa-mir-3140
MI0014163
—
chr4
152489327
152489416
−
✓


 


hsa-mir-3139
MI0014162
48
chr4
143343460
143343535
+
—


 


hsa-mir-3138
MI0014161
1
chr4
10078611
10078692
−
—


 


hsa-mir-3137
MI0014160
28
chr3
195134506
195134580
−
—


 


hsa-mir-3136
MI0014158
—
chr3
69048958
69049035
−
—


 


hsa-mir-3135b
MI0016809
71
chr6
32749912
32749979
−
—


 


hsa-mir-3135a
MI0014156
367
chr3
20137565
20137641
+
—


 


hsa-mir-3134
MI0014155
14
chr3
15697298
15697371
−
—


 


hsa-mir-3133
MI0014153
—
chr2
241477905
241477982
+
—


 


hsa-mir-3132
MI0014152
—
chr2
219549073
219549147
−
—


 


hsa-mir-3131
MI0014151
—
chr2
219058688
219058750
−
—


 


hsa-mir-3130-2
MI0014148
3
chr2
206783234
206783308
+
✓


 


hsa-mir-3130-1
MI0014147
3
chr2
206783234
206783308
−
✓


 


hsa-mir-3129
MI0014146
—
chr2
189133036
189133111
−
—


 


hsa-mir-3128
MI0014145
—
chr2
177255945
177256010
−
—


 


hsa-mir-3127
MI0014144
—
chr2
96798278
96798353
+
—


 


hsa-mir-3126
MI0014143
—
chr2
69103682
69103755
+
✓


 


hsa-mir-3125
MI0014142
9
chr2
12737367
12737444
+
—


 


hsa-mir-3124
MI0014140
—
chr1
248826377
248826443
+
—


 


hsa-mir-3123
MI0014139
46
chr1
241132272
241132346
+
—


 


hsa-mir-3122
MI0014138
—
chr1
212077613
212077685
+
—


 


hsa-mir-3121
MI0014137
—
chr1
180438314
180438390
−
—


 


hsa-mir-3120
MI0014136
—
chr1
172138808
172138888
+
—


 


hsa-mir-3119-2
MI0014135
—
chr1
170151378
170151462
+
—


 


hsa-mir-3119-1
MI0014134
—
chr1
170151378
170151462
−
—


 


hsa-mir-3118-4
MI0014207
—
chr15
21843750
21843824
+
—


 


hsa-mir-3118-3
MI0014133
—
chr15
21406385
21406459
+
—


 


hsa-mir-3118-2
MI0014132
—
chr15
20832795
20832869
+
—


 


hsa-mir-3118-1
MI0014131
—
chr21
13644775
13644850
−
—


 


hsa-mir-3117
MI0014130
—
chr1
66628440
66628517
+
—


 


hsa-mir-3116-2
MI0014129
—
chr1
62078789
62078856
−
—


 


hsa-mir-3116-1
MI0014128
—
chr1
62078786
62078859
+
—


 


hsa-mir-3115
MI0014127
29
chr1
23044305
23044372
+
—


 


hsa-mir-31
MI0000089
1263
chr9
21512115
21512185
−
✓


 


hsa-mir-30e
MI0000749
10738
chr1
40754355
40754446
+
✓


 


hsa-mir-30d
MI0000255
11691
chr8
134804876
134804945
−
✓


 


hsa-mir-30c-2
MI0000254
3223
chr6
71376960
71377031
−
✓


 


hsa-mir-30c-1
MI0000736
3205
chr1
40757284
40757372
+
✓


 


hsa-mir-30b
MI0000441
2245
chr8
134800520
134800607
−
✓


 


hsa-mir-30a
MI0000088
15057
chr6
71403551
71403621
−
✓


 


hsa-mir-3085
MI0039500
—
chr10
97875813
97875897
−
—


 


hsa-mir-3074
MI0014181
4
chr9
95086014
95086094
−
—


 


hsa-mir-3065
MI0014228
39
chr17
81125877
81125955
+
✓


 


hsa-mir-3064
MI0017375
—
chr17
64500774
64500839
−
—


 


hsa-mir-3059
MI0039499
8
chr12
86745068
86745149
−
✓


 


hsa-mir-302f
MI0006418
—
chr18
30298910
30298960
+
—


 


hsa-mir-302e
MI0006417
—
chr11
7234766
7234837
+
—


 


hsa-mir-302d
MI0000774
10
chr4
112648004
112648071
−
—


 


hsa-mir-302c
MI0000773
—
chr4
112648363
112648430
−
—


 


hsa-mir-302b
MI0000772
—
chr4
112648485
112648557
−
—


 


hsa-mir-302a
MI0000738
42
chr4
112648183
112648251
−
✓


 


hsa-mir-301b
MI0005568
140
chr22
21652981
21653058
+
✓


 


hsa-mir-301a
MI0000745
227
chr17
59151136
59151221
−
✓


 


hsa-mir-300
MI0005525
—
chr14
101041363
101041445
+
—


 


hsa-mir-29c
MI0000735
11179
chr1
207801852
207801939
−
✓


 


hsa-mir-29b-2
MI0000107
8561
chr1
207802443
207802523
−
✓


 


hsa-mir-29b-1
MI0000105
8558
chr7
130877459
130877539
−
✓


 


hsa-mir-29a
MI0000087
11796
chr7
130876747
130876810
−
✓


 


hsa-mir-299
MI0000744
14
chr14
101023794
101023856
+
✓


 


hsa-mir-298
MI0005523
—
chr20
58818226
58818313
−
—


 


hsa-mir-297
MI0005775
3
chr4
110860582
110860647
−
—


 


hsa-mir-296
MI0000747
12
chr20
58817615
58817694
−
✓


 


hsa-mir-2909
MI0013083
—
chr17
37033745
37033813
+
—


 


hsa-mir-2861
MI0013006
9
chr9
127785918
127786007
+
—


 


hsa-mir-28
MI0000086
3607
chr3
188688781
188688866
+
✓


 


hsa-mir-27b
MI0000440
14864
chr9
95085445
95085541
+
✓


 


hsa-mir-27a
MI0000085
8588
chr19
13836440
13836517
−
✓


 


hsa-mir-26b
MI0000084
17403
chr2
218402646
218402722
+
✓


 


hsa-mir-26a-2
MI0000750
36460
chr12
57824609
57824692
−
✓


 


hsa-mir-26a-1
MI0000083
26870
chr3
37969404
37969480
+
✓


 


hsa-mir-2682
MI0012063
—
chr1
98045242
98045351
−
—


 


hsa-mir-2681
MI0012062
—
chr13
101967642
101967746
−
—


 


hsa-mir-25
MI0000082
3122
chr7
100093560
100093643
−
✓


 


hsa-mir-2467
MI0017432
—
chr2
239351724
239351804
−
✓


 


hsa-mir-24-2
MI0000081
3749
chr19
13836287
13836359
−
✓


 


hsa-mir-24-1
MI0000080
3719
chr9
95086021
95086088
+
✓


 


hsa-mir-23c
MI0016010
5962
chrX
20017088
20017187
−
—


 


hsa-mir-23b
MI0000439
11091
chr9
95085208
95085304
+
—


 


hsa-mir-23a
MI0000079
10137
chr19
13836587
13836659
−
✓


 


hsa-mir-2392
MI0016870
—
chr14
100814491
100814574
+
—


 


hsa-mir-2355
MI0015873
10
chr2
207109987
207110073
−
—


 


hsa-mir-2278
MI0011285
3
chr9
94809962
94810057
+
—


 


hsa-mir-2277
MI0011284
—
chr5
93620696
93620788
−
✓


 


hsa-mir-2276
MI0011282
—
chr13
24162416
24162504
+
✓


 


hsa-mir-224
MI0000301
237
chrX
151958578
151958658
−
✓


 


hsa-mir-223
MI0000300
1044
chrX
66018870
66018979
+
✓


 


hsa-mir-222
MI0000299
3517
chrX
45747015
45747124
−
✓


 


hsa-mir-221
MI0000298
4317
chrX
45746157
45746266
−
✓


 


hsa-mir-22
MI0000078
3830
chr17
1713903
1713987
−
✓


 


hsa-mir-219b
MI0017299
—
chr9
128392621
128392708
+
✓


 


hsa-mir-219a-2
MI0000740
141
chr9
128392618
128392714
−
✓


 


hsa-mir-219a-1
MI0000296
—
chr6
33207835
33207944
+
✓


 


hsa-mir-218-2
MI0000295
1077
chr5
168768146
168768255
−
✓


 


hsa-mir-218-1
MI0000294
1151
chr4
20528275
20528384
+
✓


 


hsa-mir-217
MI0000293
120
chr2
55982967
55983076
−
—


 


hsa-mir-216b
MI0005569
5
chr2
56000714
56000795
−
—


 


hsa-mir-216a
MI0000292
413
chr2
55988950
55989059
−
✓


 


hsa-mir-215
MI0000291
686
chr1
220117853
220117962
−
—


 


hsa-mir-214
MI0000290
459
chr1
172138798
172138907
−
✓


 


hsa-mir-212
MI0000288
28
chr17
2050271
2050380
−
✓


 


hsa-mir-2117
MI0010636
—
chr17
43444806
43444885
+
—


 


hsa-mir-2116
MI0010635
—
chr15
59171183
59171262
−
✓


 


hsa-mir-2115
MI0010634
1
chr3
48316360
48316459
−
✓


 


hsa-mir-2114
MI0010633
—
chrX
150228004
150228083
+
✓


 


hsa-mir-2113
MI0003939
8
chr6
98024531
98024621
+
—


 


hsa-mir-2110
MI0010629
11
chr10
114174105
114174179
−
✓


 


hsa-mir-211
MI0000287
500
chr15
31065032
31065141
−
✓


 


hsa-mir-210
MI0000286
160
chr11
568089
568198
−
✓


 


hsa-mir-21
MI0000077
99440
chr17
59841266
59841337
+
✓


 


hsa-mir-20b
MI0001519
544
chrX
134169809
134169877
−
✓


 


hsa-mir-20a
MI0000076
7477
chr13
91351065
91351135
+
✓


 


hsa-mir-208b
MI0005570
49
chr14
23417987
23418063
−
✓


 


hsa-mir-208a
MI0000251
90
chr14
23388596
23388666
−
✓


 


hsa-mir-206
MI0000490
375
chr6
52144349
52144434
+
—


 


hsa-mir-2054
MI0010488
—
chr4
125507259
125507307
+
—


 


hsa-mir-2053
MI0010487
—
chr8
112643493
112643583
+
—


 


hsa-mir-2052
MI0010486
—
chr8
74705693
74705747
+
—


 


hsa-mir-205
MI0000285
6213
chr1
209432133
209432242
+
✓


 


hsa-mir-204
MI0000284
400
chr9
70809975
70810084
−
✓


 


hsa-mir-203b
MI0017343
—
chr14
104117418
104117503
−
—


 


hsa-mir-203a
MI0000283
524
chr14
104117405
104117514
+
✓


 


hsa-mir-202
MI0003130
190
chr10
133247511
133247620
−
✓


 


hsa-mir-200c
MI0000650
4878
chr12
6963699
6963766
+
✓


 


hsa-mir-200b
MI0000342
3832
chr1
1167104
1167198
+
✓


 


hsa-mir-200a
MI0000737
2312
chr1
1167863
1167952
+
✓


 


hsa-mir-19b-2
MI0000075
11771
chrX
134169671
134169766
−
✓


 


hsa-mir-19b-1
MI0000074
11311
chr13
91351192
91351278
+
✓


 


hsa-mir-19a
MI0000073
10618
chr13
91350891
91350972
+
✓


 


hsa-mir-199b
MI0000282
9394
chr9
128244721
128244830
−
✓


 


hsa-mir-199a-2
MI0000281
10237
chr1
172144535
172144644
−
✓


 


hsa-mir-199a-1
MI0000242
10013
chr19
10817426
10817496
−
✓


 


hsa-mir-198
MI0000240
7
chr3
120395668
120395729
−
—


 


hsa-mir-1976
MI0009986
1
chr1
26554542
26554593
+
—


 


hsa-mir-1973
MI0009983
5




—


 


hsa-mir-1972-2
MI0015977
94
chr16
70030346
70030422
+
—


 


hsa-mir-1972-1
MI0009982
94
chr16
15010321
15010397
−
—


 


hsa-mir-197
MI0000239
195
chr1
109598893
109598967
+
✓


 


hsa-mir-196b
MI0001150
1662
chr7
27169480
27169563
−
✓


 


hsa-mir-196a-2
MI0000279
1231
chr12
53991738
53991847
+
✓


 


hsa-mir-196a-1
MI0000238
1430
chr17
48632490
48632559
−
✓


 


hsa-mir-195
MI0000489
2034
chr17
7017615
7017701
−
✓


 


hsa-mir-194-2
MI0000732
155
chr11
64891355
64891439
−
✓


 


hsa-mir-194-1
MI0000488
167
chr1
220118157
220118241
−
—


 


hsa-mir-193b
MI0003137
657
chr16
14303967
14304049
+
✓


 


hsa-mir-193a
MI0000487
540
chr17
31559996
31560083
+
✓


 


hsa-mir-192
MI0000234
781
chr11
64891137
64891246
−
✓


 


hsa-mir-1915
MI0008336
3
chr10
21496562
21496641
−
—


 


hsa-mir-1914
MI0008335
—
chr20
63941465
63941544
−
—


 


hsa-mir-1913
MI0008334
2
chr6
166509354
166509433
−
—


 


hsa-mir-1912
MI0008333
8
chrX
114651544
114651623
+
✓


 


hsa-mir-1911
MI0008332
—
chrX
114763184
114763263
+
—


 


hsa-mir-1910
MI0008331
5
chr16
85741621
85741700
−
—


 


hsa-mir-191
MI0000465
8868
chr3
49020618
49020709
−
✓


 


hsa-mir-190b
MI0005545
8
chr1
154193665
154193743
−
—


 


hsa-mir-190a
MI0000486
15
chr15
62823957
62824041
+
✓


 


hsa-mir-1909
MI0008330
2
chr19
1816159
1816238
−
—


 


hsa-mir-1908
MI0008329
20
chr11
61815161
61815240
−
—


 


hsa-mir-18b
MI0001518
2965
chrX
134170041
134170111
−
✓


 


hsa-mir-18a
MI0000072
3057
chr13
91350751
91350821
+
✓


 


hsa-mir-188
MI0000484
28
chrX
50003503
50003588
+
✓


 


hsa-mir-187
MI0000274
111
chr18
35904818
35904926
−
✓


 


hsa-mir-186
MI0000483
4207
chr1
71067631
71067716
−
✓


 


hsa-mir-185
MI0000482
1871
chr22
20033139
20033220
+
✓


 


hsa-mir-1843
MI0032314
7
chr1
175968370
175968479
−
—


 


hsa-mir-184
MI0000481
127
chr15
79209788
79209871
+
✓


 


hsa-mir-183
MI0000273
490
chr7
129774905
129775014
−
✓


 


hsa-mir-1827
MI0008195
29
chr12
100189884
100189949
+
—


 


hsa-mir-1825
MI0008193
—
chr20
32237795
32237847
+
—


 


hsa-mir-182
MI0000272
1322
chr7
129770383
129770492
−
✓


 


hsa-mir-181d
MI0003139
896
chr19
13874875
13875011
+
✓


 


hsa-mir-181c
MI0000271
180
chr19
13874699
13874808
+
✓


 


hsa-mir-181b-2
MI0000683
894
chr9
124693710
124693798
+
✓


 


hsa-mir-181b-1
MI0000270
893
chr1
198858873
198858982
−
✓


 


hsa-mir-181a-2
MI0000269
3589
chr9
124692442
124692551
+
✓


 


hsa-mir-181a-1
MI0000289
3606
chr1
198859044
198859153
−
✓


 


hsa-mir-17
MI0000071
9521
chr13
91350605
91350688
+
✓


 


hsa-mir-16-2
MI0000115
10438
chr3
160404745
160404825
+
✓


 


hsa-mir-16-1
MI0000070
10589
chr13
50048973
50049061
−
✓


 


hsa-mir-15b
MI0000438
6198
chr3
160404588
160404685
+
✓


 


hsa-mir-15a
MI0000069
4045
chr13
50049119
50049201
−
✓


 


hsa-mir-1587
MI0016905
—
chrX
39837561
39837613
+
—


 


hsa-mir-155
MI0000681
355
chr21
25573980
25574044
+
✓


 


hsa-mir-154
MI0000480
2
chr14
101059755
101059838
+
✓


 


hsa-mir-1539
MI0007260
—
chr18
49487373
49487422
+
—


 


hsa-mir-1538
MI0007259
—
chr16
69565808
69565868
−
—


 


hsa-mir-1537
MI0007258
2
chr1
235853000
235853060
−
—


 


hsa-mir-153-2
MI0000464
75
chr7
157574336
157574422
−
✓


 


hsa-mir-153-1
MI0000463
69
chr2
219294111
219294200
−
✓


 


hsa-mir-152
MI0000462
2307
chr17
48037161
48037247
−
✓


 


hsa-mir-151b
MI0003772
2711
chr14
100109419
100109514
−
—


 


hsa-mir-151a
MI0000809
4889
chr8
140732564
140732653
−
—


 


hsa-mir-150
MI0000479
699
chr19
49500785
49500868
−
✓


 


hsa-mir-149
MI0000478
130
chr2
240456001
240456089
+
✓


 


hsa-mir-148b
MI0000811
2746
chr12
54337216
54337314
+
✓


 


hsa-mir-148a
MI0000253
15136
chr7
25949919
25949986
−
✓


 


hsa-mir-147b
MI0005544
32
chr15
45433050
45433129
+
✓


 


hsa-mir-147a
MI0000262
44
chr9
120244979
120245050
−
—


 


hsa-mir-1471
MI0007076
—
chr2
231892242
231892298
−
—


 


hsa-mir-1470
MI0007075
—
chr19
15449548
15449608
+
—


 


hsa-mir-146b
MI0003129
2237
chr10
102436512
102436584
+
✓


 


hsa-mir-146a
MI0000477
2369
chr5
160485352
160485450
+
✓


 


hsa-mir-1469
MI0007074
10
chr15
96333261
96333307
+
—


 


hsa-mir-1468
MI0003782
—
chrX
63786002
63786087
−
—


 


hsa-mir-145
MI0000461
29896
chr5
149430646
149430733
+
✓


 


hsa-mir-144
MI0000460
640
chr17
28861533
28861618
−
✓


 


hsa-mir-143
MI0000459
104879
chr5
149428918
149429023
+
✓


 


hsa-mir-142
MI0000458
3620
chr17
58331232
58331318
−
✓


 


hsa-mir-141
MI0000457
1700
chr12
6964097
6964191
+
✓


 


hsa-mir-140
MI0000456
5403
chr16
69933081
69933180
+
✓


 


hsa-mir-139
MI0000261
195
chr11
72615063
72615130
−
✓


 


hsa-mir-138-2
MI0000455
159
chr16
56858518
56858601
+
✓


 


hsa-mir-138-1
MI0000476
180
chr3
44114212
44114310
+
✓


 


hsa-mir-137
MI0000454
246
chr1
98046070
98046171
−
✓


 


hsa-mir-136
MI0000475
77
chr14
100884702
100884783
+
✓


 


hsa-mir-135b
MI0000810
189
chr1
205448302
205448398
−
✓


 


hsa-mir-135a-2
MI0000453
444
chr12
97563812
97563911
+
✓


 


hsa-mir-135a-1
MI0000452
590
chr3
52294219
52294308
−
✓


 


hsa-mir-1343
MI0017320
—
chr11
34941837
34941920
+
✓


 


hsa-mir-134
MI0000474
118
chr14
101054687
101054759
+
✓


 


hsa-mir-133b
MI0000822
224
chr6
52148923
52149041
+
✓


 


hsa-mir-133a-2
MI0000451
543
chr20
62564912
62565013
+
✓


 


hsa-mir-133a-1
MI0000450
543
chr18
21825698
21825785
−
✓


 


hsa-mir-1324
MI0006657
—
chr3
75630763
75630858
+
—


 


hsa-mir-1323
MI0003786
7
chr19
53671968
53672040
+
—


 


hsa-mir-1322
MI0006653
—
chr8
10825373
10825443
−
—


 


hsa-mir-1321
MI0006652
—
chrX
85835780
85835858
+
—


 


hsa-mir-132
MI0000449
751
chr17
2049908
2050008
−
✓


 


hsa-mir-130b
MI0000748
869
chr22
21653304
21653385
+
✓


 


hsa-mir-130a
MI0000448
1813
chr11
57641198
57641286
+
✓


 


hsa-mir-1307
MI0006444
249
chr10
103394253
103394401
−
✓


 


hsa-mir-1306
MI0006443
6
chr22
20086058
20086142
+
✓


 


hsa-mir-1305
MI0006372
—
chr4
182169293
182169378
+
—


 


hsa-mir-1304
MI0006371
51
chr11
93733674
93733764
−
✓


 


hsa-mir-1303
MI0006370
48
chr5
154685776
154685861
+
—


 


hsa-mir-1302-9
MI0015978
2
chr9
30144
30281
+
—


 


hsa-mir-1302-8
MI0006369
—
chr9
97363554
97363681
−
—


 


hsa-mir-1302-7
MI0006368
29
chr8
141786242
141786313
−
—


 


hsa-mir-1302-6
MI0006367
—
chr7
18127220
18127309
−
—


 


hsa-mir-1302-5
MI0006366
—
chr20
50614636
50614785
−
—


 


hsa-mir-1302-4
MI0006365
1
chr2
207269275
207269424
−
—


 


hsa-mir-1302-3
MI0006364
2
chr2
113582959
113583096
−
—


 


hsa-mir-1302-2
MI0006363
2
chr1
30366
30503
+
—


 


hsa-mir-1302-11
MI0015980
2
chr19
71973
72110
+
—


 


hsa-mir-1302-10
MI0015979
2
chr15
101960459
101960596
−
—


 


hsa-mir-1302-1
MI0006362
—
chr12
112695034
112695176
−
—


 


hsa-mir-1301
MI0003815
50
chr2
25328640
25328721
−
✓


 


hsa-mir-1299
MI0006359
6
chr9
40929010
40929092
−
—


 


hsa-mir-1298
MI0003938
2
chrX
114715233
114715344
+
✓


 


hsa-mir-1297
MI0006358
—
chr13
54311972
54312048
−
—


 


hsa-mir-1296
MI0003780
27
chr10
63372957
63373048
−
✓


 


hsa-mir-1295b
MI0019146
—
chr1
171101739
171101798
+
—


 


hsa-mir-1295a
MI0006357
1
chr1
171101728
171101806
−
—


 


hsa-mir-1294
MI0006356
6
chr5
154347106
154347247
+
✓


 


hsa-mir-1293
MI0006355
4
chr12
50234142
50234212
−
—


 


hsa-mir-1292
MI0006433
—
chr20
2652777
2652842
+
—


 


hsa-mir-1291
MI0006353
8
chr12
48654444
48654530
−
—


 


hsa-mir-1290
MI0006352
798
chr1
18897071
18897148
−
—


 


hsa-mir-129-2
MI0000473
68
chr11
43581394
43581483
+
✓


 


hsa-mir-129-1
MI0000252
69
chr7
128207872
128207943
+
✓


 


hsa-mir-1289-2
MI0006351
41
chr5
133427596
133427706
−
—


 


hsa-mir-1289-1
MI0006350
146
chr20
35453954
35454097
−
—


 


hsa-mir-1288
MI0006432
—
chr17
16282014
16282088
+
—


 


hsa-mir-1287
MI0006349
7
chr10
98395218
98395307
−
✓


 


hsa-mir-1286
MI0006348
—
chr22
20249134
20249211
−
—


 


hsa-mir-1285-2
MI0006347
17
chr2
70252918
70253005
−
—


 


hsa-mir-1285-1
MI0006346
392
chr7
92204015
92204098
−
✓


 


hsa-mir-1284
MI0006431
—
chr3
71541970
71542089
−
✓


 


hsa-mir-1283-2
MI0006430
102
chr19
53758232
53758318
+
—


 


hsa-mir-1283-1
MI0003832
114
chr19
53688481
53688567
+
—


 


hsa-mir-1282
MI0006429
115
chr15
43793659
43793759
−
—


 


hsa-mir-1281
MI0006428
—
chr22
41092513
41092566
+
—


 


hsa-mir-128-2
MI0000727
690
chr3
35744476
35744559
+
✓


 


hsa-mir-128-1
MI0000447
693
chr2
135665397
135665478
+
✓


 


hsa-mir-1279
MI0006426
—
chr12
69273157
69273218
−
—


 


hsa-mir-1278
MI0006425
—
chr1
193136503
193136583
+
✓


 


hsa-mir-1277
MI0006419
41
chrX
118386394
118386471
+
✓


 


hsa-mir-1276
MI0006416
—
chr15
85770496
85770578
−
—


 


hsa-mir-1275
MI0006415
241
chr6
33999972
34000051
−
—


 


hsa-mir-1273h
MI0025512
23
chr16
24203116
24203231
+
—


 


hsa-mir-1273c
MI0014171
12
chr6
154853360
154853436
+
✓


 


hsa-mir-1272
MI0006408
67
chr15
64762387
64762515
−
—


 


hsa-mir-1271
MI0003814
36
chr5
176367946
176368031
+
✓


 


hsa-mir-1270
MI0006407
—
chr19
20399272
20399354
−
—


 


hsa-mir-127
MI0000472
395
chr14
100882979
100883075
+
✓


 


hsa-mir-1269b
MI0016888
77
chr17
12917268
12917342
−
—


 


hsa-mir-1269a
MI0006406
82
chr4
66276824
66276928
+
✓


 


hsa-mir-1268b
MI0016748
53
chr17
80098828
80098877
+
—


 


hsa-mir-1268a
MI0006405
95
chr15
22225278
22225329
−
—


 


hsa-mir-1267
MI0006404
3
chr13
107531171
107531248
−
—


 


hsa-mir-1266
MI0006403
—
chr15
52277117
52277200
−
—


 


hsa-mir-1265
MI0006401
—
chr10
14436576
14436661
+
—


 


hsa-mir-1264
MI0003758
1
chrX
114652655
114652723
+
—


 


hsa-mir-1263
MI0006398
—
chr3
164171471
164171556
−
—


 


hsa-mir-1262
MI0006397
—
chr1
68183518
68183610
−
—


 


hsa-mir-1261
MI0006396
64
chr11
90869121
90869202
−
—


 


hsa-mir-1260b
MI0014197
913
chr11
96341438
96341526
+
—


 


hsa-mir-1260a
MI0006394
898
chr14
77266218
77266290
+
—


 


hsa-mir-126
MI0000471
4481
chr9
136670602
136670686
+
✓


 


hsa-mir-125b-2
MI0000470
11409
chr21
16590237
16590325
+
✓


 


hsa-mir-125b-1
MI0000446
13830
chr11
122099757
122099844
−
✓


 


hsa-mir-125a
MI0000469
3993
chr19
51693254
51693339
+
✓


 


hsa-mir-1258
MI0006392
—
chr2
179860836
179860908
−
—


 


hsa-mir-1257
MI0006391
8
chr20
61953546
61953662
−
—


 


hsa-mir-1256
MI0006390
—
chr1
20988314
20988432
−
✓


 


hsa-mir-1255b-2
MI0006436
4
chr1
167998660
167998726
+
—


 


hsa-mir-1255b-1
MI0006435
4
chr4
36426366
36426428
−
—


 


hsa-mir-1255a
MI0006389
6
chr4
101330302
101330414
−
✓


 


hsa-mir-1253
MI0006387
—
chr17
2748078
2748182
−
—


 


hsa-mir-1252
MI0006434
—
chr12
79419257
79419321
+
—


 


hsa-mir-1251
MI0006386
—
chr12
97491909
97491978
+
—


 


hsa-mir-1250
MI0006385
41
chr17
81133196
81133308
−
—


 


hsa-mir-1249
MI0006384
9
chr22
45200954
45201019
−
✓


 


hsa-mir-1248
MI0006383
7
chr3
186786672
186786777
+
—


 


hsa-mir-1247
MI0006382
25
chr14
101560287
101560422
−
✓


 


hsa-mir-1246
MI0006381
7472
chr2
176600980
176601052
−
—


 


hsa-mir-1245b
MI0017431
—
chr2
188978093
188978161
−
—


 


hsa-mir-1245a
MI0006380
—
chr2
188978092
188978161
+
—


 


hsa-mir-1244-4
MI0031511
—
chr12
12111952
12112036
+
—


 


hsa-mir-1244-3
MI0015975
—
chr12
9239467
9239551
−
—


 


hsa-mir-1244-2
MI0015974
—
chr5
118974586
118974670
+
—


 


hsa-mir-1244-1
MI0006379
—
chr2
231713314
231713398
+
—


 


hsa-mir-1243
MI0006373
—
chr4
113106863
113106955
+
—


 


hsa-mir-124-3
MI0000445
256
chr20
63178500
63178586
+
✓


 


hsa-mir-124-2
MI0000444
247
chr8
64379149
64379257
+
✓


 


hsa-mir-124-1
MI0000443
254
chr8
9903388
9903472
−
✓


 


hsa-mir-1238
MI0006328
—
chr19
10552122
10552204
+
—


 


hsa-mir-1237
MI0006327
—
chr11
64368602
64368703
+
—


 


hsa-mir-1236
MI0006326
—
chr6
31956839
31956940
−
—


 


hsa-mir-1234
MI0006324
1236
chr8
144400086
144400165
−
—


 


hsa-mir-1233-2
MI0015973
2
chr15
34528290
34528371
−
—


 


hsa-mir-1233-1
MI0006323
2
chr15
34382069
34382150
−
—


 


hsa-mir-1231
MI0006321
—
chr1
201808611
201808702
+
—


 


hsa-mir-122b
MI0017383
136
chr18
58451080
58451152
−
✓


 


hsa-mir-1229
MI0006319
3
chr5
179798278
179798346
−
—


 


hsa-mir-1228
MI0006318
4
chr12
57194504
57194576
+
—


 


hsa-mir-1227
MI0006316
—
chr19
2234062
2234149
−
—


 


hsa-mir-1226
MI0006313
5
chr3
47849555
47849629
+
—


 


hsa-mir-1225
MI0006311
—
chr16
2090195
2090284
−
—


 


hsa-mir-1224
MI0003764
18
chr3
184241405
184241489
+
—


 


hsa-mir-122
MI0000442
1201
chr18
58451074
58451158
+
✓


 


hsa-mir-12136
MI0039740
457
chr1
632615
632685
−
—


 


hsa-mir-12135
MI0039739
225
chr15
73299910
73299979
+
—


 


hsa-mir-12133
MI0039735
—
chr1
94543702
94543779
+
—


 


hsa-mir-12132
MI0039734
—
chr1
61846075
61846176
+
—


 


hsa-mir-12131
MI0039733
—
chr6
151102675
151102776
+
—


 


hsa-mir-12130
MI0039732
—
chr5
116255743
116255834
+
—


 


hsa-mir-12129
MI0039731
—
chrX
153335462
153335537
+
—


 


hsa-mir-12128
MI0039730
55
chr16
85207796
85207895
+
—


 


hsa-mir-12127
MI0039729
—
chr3
10280351
10280452
+
—


 


hsa-mir-12126
MI0039728
18
chr9
130241387
130241488
+
—


 


hsa-mir-12125
MI0039727
—
chr5
168165845
168165929
+
—


 


hsa-mir-12124
MI0039726
6
chr3
128846808
128846908
+
—


 


hsa-mir-12123
MI0039725
—
chr8
79332188
79332274
+
—


 


hsa-mir-12122
MI0039724
—
chr20
49863859
49863953
+
—


 


hsa-mir-12121
MI0039723
35
chr14
104865314
104865411
+
—


 


hsa-mir-12120
MI0039722
—
chrY
13479177
13479266
+
—


 


hsa-mir-12119
MI0039721
—
chr7
99409172
99409264
+
—


 


hsa-mir-12118
MI0039720
—
chr5
173303730
173303817
+
—


 


hsa-mir-12117
MI0039719
12
chr9
33108692
33108765
+
—


 


hsa-mir-12116
MI0039718
59
chr1
179803924
179804025
+
—


 


hsa-mir-12115
MI0039717
100
chr4
22515312
22515385
+
—


 


hsa-mir-12114
MI0039716
13
chr22
50407442
50407524
+
—


 


hsa-mir-12113
MI0039715
—
chr4
11783178
11783248
+
—


 


hsa-mir-1208
MI0006341
—
chr8
128150116
128150188
+
—


 


hsa-mir-1207
MI0006340
—
chr8
128049152
128049238
+
—


 


hsa-mir-1206
MI0006339
—
chr8
128008898
128008956
+
—


 


hsa-mir-1205
MI0006338
—
chr8
127960633
127960695
+
—


 


hsa-mir-1204
MI0006337
—
chr8
127795962
127796028
+
—


 


hsa-mir-1203
MI0006335
—
chr17
48156427
48156511
−
—


 


hsa-mir-1202
MI0006334
28
chr6
155946797
155946879
+
—


 


hsa-mir-1200
MI0006332
—
chr7
36919357
36919432
−
—


 


hsa-mir-1199
MI0020340
—
chr19
14073361
14073479
+
—


 


hsa-mir-1197
MI0006656
—
chr14
101025564
101025651
+
—


 


hsa-mir-1193
MI0014205
52
chr14
101030052
101030129
+
—


 


hsa-mir-1185-2
MI0003821
2
chr14
101044198
101044283
+
✓


 


hsa-mir-1185-1
MI0003844
2
chr14
101042977
101043062
+
✓


 


hsa-mir-1184-3
MI0015972
180
chrX
155457517
155457615
+
—


 


hsa-mir-1184-2
MI0015971
180
chrX
155383100
155383198
−
—


 


hsa-mir-1184-1
MI0006277
180
chrX
154887360
154887458
−
—


 


hsa-mir-1183
MI0006276
—
chr7
21471058
21471146
+
—


 


hsa-mir-1182
MI0006275
—
chr1
231019828
231019924
−
—


 


hsa-mir-1181
MI0006274
3
chr19
10403458
10403538
−
—


 


hsa-mir-1180
MI0006273
103
chr17
19344506
19344574
−
✓


 


hsa-mir-1179
MI0006272
—
chr15
88608107
88608197
+
—


 


hsa-mir-1178
MI0006271
—
chr12
119713634
119713724
−
—


 


hsa-mir-11401
MI0036560
8
chr16
87853896
87853964
+
—


 


hsa-mir-11400
MI0036559
12
chr7
142474527
142474603
+
—


 


hsa-mir-11399
MI0036558
—
chr1
112882050
112882129
+
—


 


hsa-mir-11181
MI0035972
—
chr15
87946206
87946268
−
—


 


hsa-mir-10b
MI0000267
7790
chr2
176150303
176150412
+
—


 


hsa-mir-10a
MI0000266
5181
chr17
48579838
48579947
−
✓


 


hsa-mir-107
MI0000114
11858
chr10
89592747
89592827
−
✓


 


hsa-mir-106b
MI0000734
1841
chr7
100093993
100094074
−
✓


 


hsa-mir-106a
MI0000113
7942
chrX
134170198
134170278
−
✓


 


hsa-mir-10527
MI0033674
2
chr12
63823663
63823727
+
—


 


hsa-mir-10526
MI0033673
—
chr11
122152103
122152156
−
—


 


hsa-mir-10525
MI0033672
—
chr7
74595968
74596026
+
—


 


hsa-mir-10524
MI0033671
12
chr6
78539263
78539309
−
—


 


hsa-mir-10523
MI0033669
36
chr5
172915083
172915158
+
—


 


hsa-mir-10522
MI0033668
—
chr5
17155930
17155992
−
—


 


hsa-mir-105-2
MI0000112
15
chrX
152394412
152394492
−
✓


 


hsa-mir-105-1
MI0000111
15
chrX
152392219
152392299
−
✓


 


hsa-mir-10401
MI0033425
156
chr21
8206563
8206618
+
—


 


hsa-mir-10400
MI0033424
16
chr8
144462485
144462539
−
—


 


hsa-mir-103b-2
MI0007262
—
chr20
3917502
3917563
−
—


 


hsa-mir-103b-1
MI0007261
233
chr5
168560904
168560965
+
—


 


hsa-mir-103a-2
MI0000108
12432
chr20
3917494
3917571
+
✓


 


hsa-mir-103a-1
MI0000109
12432
chr5
168560896
168560973
−
✓


 


hsa-mir-10399
MI0033423
19
chr7
139044100
139044157
−
—


 


hsa-mir-10398
MI0033422
8
chr6
41733554
41733610
−
—


 


hsa-mir-10397
MI0033421
—
chr5
10402364
10402431
+
—


 


hsa-mir-10396b
MI0033426
—
chr21
8436324
8436374
+
✓


 


hsa-mir-10396a
MI0033420
—
chr21
8987617
8987665
+
—


 


hsa-mir-10395
MI0033419
—
chr19
12703601
12703664
−
—


 


hsa-mir-10394
MI0033418
—
chr19
58393364
58393446
+
—


 


hsa-mir-10393
MI0033417
—
chr15
44717844
44717893
+
—


 


hsa-mir-10392
MI0033416
8
chr11
64878523
64878609
−
—


 


hsa-mir-10226
MI0033056
169
chr17
48032882
48032951
+
—


 


hsa-mir-101-2
MI0000739
6084
chr9
4850297
4850375
+
✓


 


hsa-mir-101-1
MI0000103
6056
chr1
65058434
65058508
−
✓


 


hsa-mir-100
MI0000102
10013
chr11
122152229
122152308
−
✓


 


hsa-mir-1-2
MI0000437
13316
chr18
21829004
21829088
−
—


 


hsa-mir-1-1
MI0000651
6511
chr20
62554306
62554376
+
✓


 


hsa-let-7i
MI0000434
17040
chr12
62603686
62603769
+
✓


 


hsa-let-7g
MI0000433
102832
chr3
52268278
52268361
−
✓


 


hsa-let-7f-2
MI0000068
118700
chrX
53557192
53557274
−
✓


 


hsa-let-7f-1
MI0000067
109279
chr9
94176347
94176433
+
✓


 


hsa-let-7e
MI0000066
50938
chr19
51692786
51692864
+
✓


 


hsa-let-7d
MI0000065
4649
chr9
94178834
94178920
+
✓


 


hsa-let-7c
MI0000064
135622
chr21
16539828
16539911
+
✓


 


hsa-let-7b
MI0000063
83403
chr22
46113686
46113768
+
✓


 


hsa-let-7a-3
MI0000062
142757
chr22
46112749
46112822
+
✓


 


hsa-let-7a-2
MI0000061
142652
chr11
122146522
122146593
−
✓


 


hsa-let-7a-1
MI0000060
145261
chr9
94175957
94176036
+
✓









AAV

Recombinant AAV genomes of the disclosure comprise a disclosed nucleic acid molecule and one or more AAV ITRs flanking a nucleic acid molecule. AAV DNA in the rAAV genomes may be from any AAV serotype for which a recombinant virus can be derived including, but not limited to, AAV serotypes AAV-B1, AAVrh.74, AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-7, AAV-8, AAV-9, AAV-10, AAV-11, AAV-12 and AAV-13. Production of pseudotyped rAAV is disclosed in, for example, WO 01/83692. Other types of rAAV variants, for example rAAV with capsid mutations, are also contemplated. See, for example, Marsic et al., Molecular Therapy, 22(11): 1900-1909 (2014). As noted in the Background section above, the nucleotide sequences of the genomes of various AAV serotypes are known in the art. To promote skeletal muscle specific expression, AAV1, AAV5, AAV6, AAV8 or AAV9 may be used.
Self-complementary AAV (scAAV) vectors are also contemplated for use in the present disclosure. scAAV vectors are generated by reducing the vector size to approximately 2500 base pairs, which comprise 2200 base pairs of unique transgene sequence plus two copies of the 145 base pair ITR packaged as a dimer. The scAAV have the ability to re-fold into double stranded DNA templates for expression. McCarthy, Mol. Therap. 16(10): 1648-1656, 2008.
DNA plasmids of the disclosure comprise a rAAV genome. The DNA plasmids are transferred to cells permissible for infection with a helper virus of AAV (e.g., adenovirus, El-deleted adenovirus or herpesvirus) for assembly of the rAAV genome into infectious viral particles. Techniques to produce rAAV particles, in which an AAV genome to be packaged, rep and cap genes, and helper virus functions are provided to a cell, are known in the art. Production of rAAV requires that the following components are present within a single cell (denoted herein as a packaging cell): a rAAV genome, AAV rep and cap genes separate from (i.e., not in) the rAAV genome, and helper virus functions. The AAV rep and cap genes may be from any AAV serotype for which recombinant virus can be derived and may be from a different AAV serotype than the rAAV genome ITRs, including, but not limited to, AAV serotypes AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-7, AAV-8, AAV-9, AAV-10, AAV-11, AAV-12 and AAV-13. Production of pseudotyped rAAV is disclosed in, for example, WO 01/83692 which is incorporated by reference herein in its entirety.
A method of generating a packaging cell is to create a cell line that stably expresses all the necessary components for AAV particle production. For example, a plasmid (or multiple plasmids) comprising a rAAV genome lacking AAV rep and cap genes, AAV rep and cap genes separate from the rAAV genome, and a selectable marker, such as a neomycin resistance gene, are integrated into the genome of a cell. AAV genomes have been introduced into bacterial plasmids by procedures such as GC tailing (Samulski et al., 1982, Proc. Natl. Acad. S6. USA, 79:2077-2081), addition of synthetic linkers containing restriction endonuclease cleavage sites (Laughlin et al., 1983, Gene, 23:65-73) or by direct, blunt-end ligation (Senapathy & Carter, 1984, J. Biol. Chem., 259:4661-4666). The packaging cell line is then infected with a helper virus such as adenovirus. The advantages of this method are that the cells are selectable and are suitable for large-scale production of rAAV. Other examples of suitable methods employ adenovirus or baculovirus rather than plasmids to introduce rAAV genomes and/or rep and cap genes into packaging cells.
General principles of rAAV production are reviewed in, for example, Carter, 1992, Current Opinions in Biotechnology, 1533-539; and Muzyczka, 1992, Curr. Topics in Microbial. and Immunol., 158:97-129). Various approaches are described in Ratschin et al., Mol. Cell. Biol. 4:2072 (1984); Hermonat et al., Proc. Natl. Acad. Sci. USA, 81:6466 (1984); Tratschin et al., Mol. Cell. Biol. 5:3251 (1985); McLaughlin et al., J. Virol., 62:1963 (1988); and Lebkowski et al., 1988 Mol. Cell. Biol., 7:349 (1988). Samulski et al. (1989, J. Virol., 63:3822-3828); U.S. Pat. No. 5,173,414; WO 95/13365 and corresponding U.S. Pat. No. 5,658.776; WO 95/13392; WO 96/17947; PCT/US98/18600; WO 97/09441 (PCT/US96/14423); WO 97/08298 (PCT/US96/13872); WO 97/21825 (PCT/US96/20777); WO 97/06243 (PCT/FR96/01064); WO 99/11764; Perrin et al. (1995) Vaccine 13:1244-1250; Paul et al. (1993) Human Gene Therapy 4:609-615; Clark et al. (1996) Gene Therapy 3:1124-1132; U.S. Patent. No. 5,786,211; U.S. Patent No. 5,871,982; and U.S. Pat No. 6,258,595. The foregoing documents are hereby incorporated by reference in their entirety herein, with particular emphasis on those sections of the documents relating to rAAV production.
The disclosure thus provides packaging cells that produce infectious rAAV. In one embodiment packaging cells may be stably transformed cancer cells such as HeLa cells, 293 cells and PerC.6 cells (a cognate 293 line). In another embodiment, packaging cells are cells that are not transformed cancer cells, such as low passage 293 cells (human fetal kidney cells transformed with El of adenovirus), MRC-5 cells (human fetal fibroblasts), WI-38 cells (human fetal fibroblasts), Vero cells (monkey kidney cells) and FRhL-2 cells (rhesus fetal lung cells).
Recombinant AAV (i.e., infectious encapsidated rAAV particles) of the disclosure comprise a rAAV genome. Embodiments include, but are not limited to, the rAAV named “AAV.miDUX4.405” including a genome encoding the DUX4 miRNA hDux.mi405 (encoded by the DNA set out in SEQ ID NO: 1 and the rAAV named “AAV.miDUX4.1155” including a genome encoding the DUX4 miRNA hDux.mil155 (encoded by the DNA set out in SEQ ID NO: 2). In exemplary embodiments, the genomes of both rAAV lack AAV rep and cap DNA, that is, there is no AAV rep or cap DNA between the ITRs of the genomes. Examples of rAAV that may be constructed to comprise the nucleic acid molecules of the disclosure are set out in International Patent Application No. PCT/US2012/047999 (WO 2013/016352) incorporated by reference herein in its entirety.
The rAAV may be purified by methods such as by column chromatography or cesium chloride gradients. Methods for purifying rAAV vectors from helper virus are known in the art and include methods disclosed in, for example, Clark et al., Hum. Gene Ther., 10(6): 1031-1039 (1999); Schenpp and Clark, Methods Mol. Med., 69 427-443 (2002); U.S. Pat. No. 6,566,118 and WO 98/09657.
In another embodiment, the disclosure contemplates compositions comprising a disclosed rAAV. Compositions of the disclosure comprise rAAV in a pharmaceutically acceptable carrier. The compositions may also comprise other ingredients such as diluents and adjuvants. Acceptable carriers, diluents and adjuvants are nontoxic to recipients and are preferably inert at the dosages and concentrations employed, and include buffers such as phosphate, citrate, or other organic acids; antioxidants such as ascorbic acid; low molecular weight polypeptides; proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, arginine or lysine; monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugar alcohols such as mannitol or sorbitol; salt-forming counterions such as sodium; and/or nonionic surfactants such as Tween, pluronics or polyethylene glycol (PEG).
Titers of rAAV to be administered in methods of the disclosure will vary depending, for example, on the particular rAAV, the mode of administration, the treatment goal, the individual, and the cell type(s) being targeted, and may be determined by methods known in the art. Titers of rAAV may range from about 1×106, about 1×107, about 1×108, about 1×109, about 1×1010, about 1×1011, about 1×1012, about 1×1013 to about 1×1014 or more DNase resistant particles (DRP) per ml. Dosages may also be expressed in units of viral genomes (vg).
Methods of transducing a target cell with rAAV, in vivo or in vitro, are contemplated by the disclosure. The in vivo methods comprise the step of administering an effective dose, or effective multiple doses, of a composition comprising a rAAV of the disclosure to an animal (including a human being) in need thereof. If the dose is administered prior to development of a disorder/disease, the administration is prophylactic. If the dose is administered after the development of a disorder/disease, the administration is therapeutic. In embodiments of the disclosure, an effective dose is a dose that alleviates (eliminates or reduces) at least one symptom associated with the disorder/disease state being treated, that slows or prevents progression to a disorder/disease state, that slows or prevents progression of a disorder/disease state, that diminishes the extent of disease, that results in remission (partial or total) of disease, and/or that prolongs survival. An example of a disease contemplated for prevention or treatment with the disclosed methods is FSHD.
Combination therapies are also contemplated by the disclosure. Combination as used herein includes both simultaneous treatment and sequential treatments. Combinations of methods of the disclosure with standard medical treatments (e.g., corticosteroids) are specifically contemplated, as are combinations with novel therapies.
Administration of an effective dose of the compositions may be by routes known in the art including, but not limited to, intramuscular, parenteral, intravenous, oral, buccal, nasal, pulmonary, intracranial, intraosseous, intraocular, rectal, or vaginal. Route(s) of administration and serotype(s) of AAV components of the rAAV (in particular, the AAV ITRs and capsid protein) of the disclosure may be chosen and/or matched by those skilled in the art taking into account the infection and/or disease state being treated and the target cells/tissue(s) that are to express the DUX4 miRNAs.
The disclosure provides for local administration and systemic administration of an effective dose of recombinant AAV and compositions of the disclosure. For example, systemic administration is administration into the circulatory system so that the entire body is affected. Systemic administration includes enteral administration such as absorption through the gastrointestinal tract and parental administration through injection, infusion or implantation.
In particular, actual administration of rAAV of the present disclosure may be accomplished by using any physical method that will transport the rAAV recombinant vector into the target tissue of an animal. Administration according to the disclosure includes, but is not limited to, injection into muscle, the bloodstream and/or directly into the liver. Simply resuspending a rAAV in phosphate buffered saline has been demonstrated to be sufficient to provide a vehicle useful for muscle tissue expression, and there are no known restrictions on the carriers or other components that can be co-administered with the rAAV (although compositions that degrade DNA should be avoided in the normal manner with rAAV). Capsid proteins of a rAAV may be modified so that the rAAV is targeted to a particular target tissue of interest such as muscle. See, for example, WO 02/053703, the disclosure of which is incorporated by reference herein. Pharmaceutical compositions can be prepared as injectable formulations or as topical formulations to be delivered to the muscles by transdermal transport. Numerous formulations for both intramuscular injection and transdermal transport have been previously developed and can be used in the practice of the disclosed methods and compositions. The rAAV can be used with any pharmaceutically acceptable carrier for ease of administration and handling.
For purposes of intramuscular injection, solutions in an adjuvant such as sesame or peanut oil or in aqueous propylene glycol can be employed, as well as sterile aqueous solutions. Such aqueous solutions can be buffered, if desired, and the liquid diluent first rendered isotonic with saline or glucose. Solutions of rAAV as a free acid (DNA contains acidic phosphate groups) or a pharmacologically acceptable salt can be prepared in water suitably mixed with a surfactant such as hydroxpropylcellulose. A dispersion of rAAV can also be prepared in glycerol, liquid polyethylene glycols and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms. In this connection, the sterile aqueous media employed are all readily obtainable by techniques known to those skilled in the art.
The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating actions of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, liquid polyethylene glycol and the like), suitable mixtures thereof, and vegetable oils. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of a dispersion and by the use of surfactants. The prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal and the like. In many cases it will be preferable to include isotonic agents, for example, sugars or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by use of agents delaying absorption, for example, aluminum monostearate and gelatin.
Sterile injectable solutions are prepared by incorporating rAAV in the required amount in the appropriate solvent with various other ingredients enumerated above, as required, followed by filter sterilization. Generally, dispersions are prepared by incorporating the sterilized active ingredient into a sterile vehicle which contains the basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and the freeze drying technique that yield a powder of the active ingredient plus any additional desired ingredient from the previously sterile-filtered solution thereof.
Transduction with rAAV may also be carried out in vitro. In one embodiment, desired target muscle cells are removed from the subject, transduced with rAAV and reintroduced into the subject. Alternatively, syngeneic or xenogeneic muscle cells can be used where those cells will not generate an inappropriate immune response in the subject.
Suitable methods for the transduction and reintroduction of transduced cells into a subject are known in the art. In one embodiment, cells can be transduced in vitro by combining rAAV with muscle cells, e.g., in appropriate media, and screening for those cells harboring the DNA of interest using techniques such as Southern blots and/or PCR, or by using selectable markers. Transduced cells can then be formulated into pharmaceutical compositions, and the composition introduced into the subject by various techniques, such as by intramuscular, intravenous, subcutaneous and intraperitoneal injection, or by injection into smooth and cardiac muscle, using e.g., a catheter.
Transduction of cells with rAAV of the disclosure results in sustained expression of DUX4 miRNAs. The present disclosure thus provides methods of administering/delivering rAAV which express DUX4 miRNAs to an animal, preferably a human being. These methods include transducing tissues (including, but not limited to, tissues such as muscle, organs such as liver and brain, and glands such as salivary glands) with one or more disclosed rAAV. Transduction may be carried out with gene cassettes comprising tissue specific control elements. For example, one embodiment provides methods of transducing muscle cells and muscle tissues directed by muscle specific control elements, including, but not limited to, those derived from the actin and myosin gene families, such as from the myoD gene family [See Weintraub et al., Science, 251: 761-766 (1991)], the myocyte-specific enhancer binding factor MEF-2 [Cserjesi and Olson, Mol Cell Biol 11: 4854-4862 (1991)], control elements derived from the human skeletal actin gene [Muscat et al., Mol Cell Biol, 7: 4089-4099 (1987)], the cardiac actin gene, muscle creatine kinase sequence elements [See Johnson et al., Mol Cell Biol, 9:3393-3399 (1989)] and the murine creatine kinase enhancer (mCK) element, control elements derived from the skeletal fast-twitch troponin C gene, the slow-twitch cardiac troponin C gene and the slow-twitch troponin I gene: hypoxia-inducible nuclear factors (Semenza et al., Proc Natl Acad Sci USA, 88: 5680-5684 (1991)), steroid-inducible elements and promoters including the glucocorticoid response element (GRE) (See Mader and White, Proc. Nall. Acad. Sci. USA 90: 5603-5607 (1993)), and other control elements.
Muscle tissue is an attractive target for in vivo DNA delivery, because it is not a vital organ and is easy to access. The disclosure contemplates sustained expression of miRNAs from transduced myofibers.
By “muscle cell” or “muscle tissue” is meant a cell or group of cells derived from muscle of any kind (for example, skeletal muscle and smooth muscle, e.g. from the digestive tract, urinary bladder, blood vessels or cardiac tissue). Such muscle cells may be differentiated or undifferentiated, such as myoblasts, myocytes, myotubes, cardiomyocytes and cardiomyoblasts.
The term “transduction” is used to refer to the administration/delivery of DUX4 miRNAs to a recipient cell either in vivo or in vitro, via a replication-deficient rAAV resulting in expression of a DUX4 miRNA by the recipient cell.
Thus, the disclosure provides methods of administering an effective dose (or doses, administered essentially simultaneously or doses given at intervals) of rAAV that encode DUX4 miRNAs to a patient in need thereof.
DUX4 and Facioscapulohumeral Muscular Dystrophy
Muscular dystrophies (MDs) are a group of genetic diseases. The group is characterized by progressive weakness and degeneration of the skeletal muscles that control movement or breathing. Some forms of MD develop in infancy or childhood, while others may not appear until middle age or later. The disorders differ in terms of the distribution and extent of muscle weakness (some forms of MD also affect cardiac muscle), the age of onset, the rate of progression, and the pattern of inheritance.
Facioscapulohumeral muscular dystrophy (FSHD) is a complex autosomal dominant disorder characterized by progressive and asymmetric weakness of facial, shoulder and limb muscles. Symptoms typically arise in adulthood with most patients showing clinical features before age thirty. About five percent of patients develop symptoms as infants or juveniles and these are generally more severely affected. Clinical presentation can vary from mild (some limited muscle weakness) to severe (wheelchair dependence). Historically, FSHD was classified as the third most common MD, affecting one in 20,000 individuals worldwide. However, recent data indicate FSHD is the most common MD in Europe, suggesting its worldwide incidence could be as high as 1 in 8,333.
Typical FSHD cases (FSHD1A, heretofore referred to as FSHD) are linked to heterozygous chromosomal deletions that decrease the copy number of 3.3 kilobase (kb) D4Z4 repeats on human chromosome 4q35. Simplistically, normal individuals have 11-100 tandemly-repeated D4Z4 copies on both 4q35 alleles, while patients with FSHD have one normal and one contracted allele containing 1-10 repeats. In addition, FSHD-associated D4Z4 contractions must occur on specific disease-permissive chromosome 4q35 backgrounds (called 4qA). Importantly, no genes are completely lost or structurally mutated as a result of FSHD-associated deletions. Instead, genetic changes associated with FSHD give rise to expression of the toxic DUX4 gene, which is damaging to muscle. FSHD2 (also known as FSHD1B) is phenotypically identical to FSHD1, is associated with DUX4 expression, and requires the 4qA chromosomal background. FSHD2 is not associated with D4Z4 repeat contraction, but is instead caused by mutation in the SMCHD1 gene, which is a chromatin regulator normally involved in repressing the DUX4 locus at 4qA. Mutated SMCHD1 proteins fail to participate in adding heterochromatin to the 4qA DUX4 allele, thereby allowing DUX4 gene expression.
In the leading FSHD pathogenesis model, D4Z4 contractions are proposed to cause epigenetic changes that permit expression of the DUX4 gene. As a result, the aberrant over-expression of otherwise silent or near-silent DUX4 gene, and the genes it regulates, may ultimately cause FSHD. This model is consistent with data showing normal 4q35 D4Z4 repeats have heterochromatin characteristics, while FSHD-linked D4Z4 repeats contain marks more indicative of actively transcribed euchromatin. These transcription-permissive epigenetic changes, coupled with the observation that complete monosomic D4Z4 deletions (i.e., zero repeats) do not cause FSHD, support the hypothesis that D4Z4 repeats harbor potentially myopathic open reading frames (ORFs), which are abnormally expressed in FSHD muscles. This notion was initially considered in 1994, when a D4Z4-localized ORF, called DUX4, was first identified. However, the locus had some characteristics of an unexpressed pseudogene and DUX4 was therefore summarily dismissed as an FSHD candidate. For many years thereafter, the search for FSHD-related genes was mainly focused outside the D4Z4 repeats, and although some intriguing candidates emerged from these studies, no single gene had been conclusively linked to FSHD development. This slow progress led to the re-emergence of DUX4 as an FSHD candidate in 2007. Even as of 2010 though, researchers continued to highlight other genes as candidates. See, for example, Wuebbles et al., Int. J. Clin. Exp. Pathol., 3(4): 386-400 (2010) highlighting the FSHD region gene 1 (frg1). In contrast, Wallace et al., Mol. Ther., 17(Suppl. 1): S151 (2009); Wei et al., Mol. Ther., 17(Suppl. 1): S200 (2009); and the Lemmers et al. report from the Sciencexpress issue of Aug. 19, 2010 highlight DUX4. Neguembor and Gabellini, Epigenomics, 2(2): 271-287 (2010) is a recent review article regarding FSHD.
The role of DUX4 in FSHD pathogenesis can be explained as follows. First, D4Z4 repeats contain identical DUX4 coding regions, and D4Z4 repeats also harbor smaller sense and antisense transcripts, including some resembling microRNAs. Over-expressed DUX4 transcripts and a ˜50 kDa full-length DUX4 protein are found in biopsies and cell lines from FSHD patients. These data are consistent with a transcriptional de-repression model of FSHD pathogenesis. In addition, unlike pseudogenes, D4Z4 repeats and DUX4 likely have functional importance, since tandemly-arrayed D4Z4 repeats are conserved in at least eleven different placental mammalian species (non-placental animals lack D4Z4 repeats), with the greatest sequence conservation occurring within the DUX4 ORF. Second, over-expressed DUX4 is toxic to tissue culture cells and embryonic progenitors of developing lower organisms in vivo. This toxicity occurs at least partly through a pro-apoptotic mechanism, indicated by Caspase-3 activation in DUX4 transfected cells, and presence of TUNEL-positive nuclei in developmentally arrested Xenopus embryos injected with DUX4 mRNA at the two-cell stage. These findings are consistent with studies showing some pro-apoptotic proteins, including Caspase-3, are present in FSHD patient muscles. In addition to stimulating apoptosis, DUX4 may negatively regulate myogenesis. Human DUX4 inhibits differentiation of mouse C2C12 myoblasts in vitro, potentially by interfering with PAX3 and/or PAX7, and causes developmental arrest and reduced staining of some muscle markers when delivered to progenitor cells of zebrafish or Xenopus embryos. Finally, aberrant DUX4 function is directly associated with potentially important molecular changes seen in FSHD patient muscles. Specifically, full-length human DUX4 encodes an approximately 50 kDa double homeodomain transcription factor, and DUX4 targets can be found at elevated levels in FSHD patient muscles. These data support that DUX4 catalyzes numerous downstream molecular changes that are incompatible with maintaining normal muscle integrity.



BRIEF DESCRIPTION OF THE DRAWING
FIG. 1 shows the wild type U6-1 promoter (SEQ ID NO: 3)
FIGS. 2A and 2B set out sequences of DUX4 targeted miRNAs. In each panel, the top sequences indicate the DNA templates from which each respective miRNA is transcribed. In the top panel, the DNA template miDUX4.405 (miDUX4-1 or mi405) is SEQ ID NO: 1. In the bottom panel, the DNA template miDUX4.1155 (miDUX4-2; or mi1155) is SEQ ID NO: 2. The folded miRNA transcripts are shown as hairpin structures. The miDUX4.405 folded miRNAs is SEQ ID NO: 8. The miDUX4.1155 folded miRNA is SEQ ID NO: 9. The mature miDUX4.405 and miDUX4.1155 sequences arise following processing in target cells by host miRNA processing machinery (including Drosha, DGCR8, Dicer, and Exportin-5). Sequences shaded in gray indicate restriction sites used for cloning each miRNA into the U6T6 vector. CTCGAG is an XhoI site and ACTAGT is a SpeI site (CUCGAG and ACUAGU in RNA, where the U is a uracil base). The red sequence indicates the mature miRNA antisense guide strand that ultimately helps catalyze cleavage of the DUX4 target mRNA. This sequence is also underlined in the miRNA hairpin portions of this diagram. The gray and black arrowheads indicate Drosha- and Dicer- catalyzed cleavage sites, respectively. The numbers 13, 35, 53, and 75 are provided for orientation. The sequences between (and including) positions 35-53 are derived from the natural human mir-30a sequence, except the A at position 39, which is a G is the normal mir-30a sequence. This nucleotide was changed to an A to facilitate folding of the miRNA loop, based on in silico RNA folding models. The base of the stem (5′ of position 13 and 3′ of position 75) is also derived from mir-30a structure and sequence with some modifications depending on the primary sequence of the guide strand. Specifically, the nucleotide at position 13 can vary to help facilitate a required mismatched between the position 13 and 75 nucleotides. This bulged structure is hypothesized to facilitate proper Drosha cleavage.
FIG. 3 shows the strategy for de-targeting miDUX4 in heart and liver. The perfect binding sites for mir-122 (liver) and mir-208 (heart) are indicated in the figure. Evidence that mir-122-modified miDUX4 is functional against a DUX4-luciferase target, and that liver cells expressing mir-122 can inhibit miDUX4 silencing when mir-122 binding sites are included in the miDUX4 sequence.
FIG. 4 shows the human DUX4 DNA sequence (SEQ ID NO: 7).



SEQUENCES
SEQ ID NO: 1 (miDUX4.405 or miDUX4-1)
SEQ ID NO: 2 (miDUX4.1155 or miDUX4-2)
SEQ ID NOS: 10-10912, 10971, 10972: Exemplary miRNA mature guide strand nucleotide sequences
SEQ ID NO: 3 wild type U6-1 promoter
SEQ ID NO: 4 weakened U6-1 promoter with mutations within the PSE region.
SEQ ID NO: 5 Binding site for miR-122 (5′ TATTTAGTGTGAT AATGGTGTTT 3′)
SEQ ID NO: 6—Binding site for miRNA-208 (5′ ACGAGCcTTTT GCTCGTCTTAT 3′)
SEQ ID NO: 8—miDUX4.405 (miDUX4-1) folded miRNA
SEQ ID NO: 9—miDUX4.1155 (miDUX4-2) folded miRNA
SEQ ID NO: 7—DUX4 gene sequence
SEQ ID NOS: 10913-10968 Exemplary nucleic acid sequences comprising the mature guide strand of miDUX4 and a binding site for miR-122 or miR-208 (also shown in Table 1)
SEQ ID NO: 10969—Binding site for miR-122 (5′ UAUUUAGU GUGAUAAUGGUGUUU 3′)
SEQ ID NO: 10970—Binding site for miR-208 (5′ ACGAGCcUUUU GCUCGUCUUAU 3′)
SEQ ID NO: 10973—miDUX4.405 (miDUX4-1) mature guide strand nucleotide sequence
SEQ ID NO: 10974—miDUX4.1155 (miDUX4-2) mature guide strand nucleotide sequence
When mature guide stand sequences are presented as DNA sequences herein, one of skill in the art understands that this DNA sequence serves as a template for transcription to RNA wherein the thymidine bases are converted to uridine bases.
Thus, aspects and embodiments of the disclosure are illustrated by the following examples. Example 1 describes the liver and heart detargeted, U6 promoter system. Example 2 describes the luciferase assay for determining the effect of the miRNAs expression of DUX4 miRNAs. Example 3 describes rAAV vectors encoding DUX4 miRNAs.
EXAMPLE 1
Liver and Heart De-Targeted, U6 Promoter System
AAV vectors expressing the miDUX4.405 sequence (SEQ ID NO: 1) from the wild-type U6 promoter were generated. Muscles were co-injected with AAV.DUX4. The wild-type U6 system protected muscles from damage resulting from high DUX4 expression.
One option for skeletal muscle specific expression is to use the AAV6 vector, as it primarily transduces skeletal muscle, liver, and heart following vascular delivery, and significantly less in other tissues. To avoid expression in liver and heart, the U6 promoter system detargets miDUX4 in those tissues. To do this, binding sites for mir-122 and mir-208) (liver- and heart-specific natural microRNAs) are incorporated at various locations within the miDUX4 transcript as shown in FIG. 3. The de-targeted miDUX4 transcripts expression are measured, using the DUX4-luciferase target described below in Example 2.
EXAMPLE 2
Luciferase Assay for Effect of Expression of DUX4 miRNAs
Expression of the DUX4 target sequence in the presence of the DUX4 miRNAs is assayed. A lipofectamine 2000 transfection is done in 293 cells in a 96-well, white-walled assay plate. 140,000 cells are transfected with 20 ng of a Renilla-firefly plasmid containing the DUX4 target sequence and 180 ng of various DUX4 miRNA-encoding vectors, including U6T6-driven miDux4.405 or miDux4.1155 vectors from Example 1. A luciferase assay is performed 24 hours later.
The media is removed from the cells and 20 μl of lysis buffer was added per well. The plate is put on a shaker for 15 minutes at room temperature before adding 50 μl of luciferase substrate. The first reading is taken 10 minutes later. Next, 50 μl of Stop and Glo luciferase substrate is added and the second reading is taken 10 minutes later. The Renilla expression is divided by the firefly expression to calculate the relative expression. The relative expression is then normalized to the expression of cells that were transfected with a control miRNA that targets eGFP. The DUX4 miRNAs miDUX4.405 and miDUX4.1155 are the most effective at reducing luciferase protein expression in transfected cells. The de-targeted miDUX4 transcripts are destroyed by mir-122 and mir-208 RISC complexes in the liver and heart, respectively, using the DUX4-luciferase target described below in Example 1.
EXAMPLE 3
Production of rAAV Encoding DUX4 MicroRNAs
Vector is produced by co-transfection in HEK293 cells of three plasmids (pAdhelper, AAV helper, and the rAAV genome containing miDUX4; described in detail below), followed by cell-harvesting, vector purification, titration, and quality control assays.
Plasmids: pAdhelper contains the adenovirus genes E2A, E4 ORF6, and VA I/II; AAV helper plasmids contain AAV rep2 and cap6 (for example, for an AAV serotype 6 preparation, the capsid gene would be called cap6); the rAAV plasmid contains AAV inverted terminal repeat (ITRs) sequences flanking the genetic elements to be packaged into the vector. For the AAV.miDUX4, this includes the U6.miDUX4 cloned upstream of the CMV.eGFP reporter gene.
Transfection: Plasmids are transfected into 293 cells (Corning 10-Stack) using CaPO4 at a 4:4:1 ratio (20 μg pAd helper: 20 μg AAV helper: 5 ug rAAV vector plasmid per plate.
Cell harvesting: Forty-eight hr post-transfection, cells are harvested and resuspended in 20 mM Tris (pH 8.0), 1 mM MgCl2 and 150 mM NaCl (T20M1N150) at a density of 5×106 cells/ml. Cells are lysed by four sequential freeze/thaw cycles and Benzonase nuclease (AIC, Stock: 250 U/μ1) added to a final concentration of 90 U/ml before cell lysate clarification.
Vector Purification and Titration: Clarified lysates are subjected to iodixanol step gradient purification as previously described (Xiao, X, et al. J. Virol 72:2224-32). The 40% iodixanol layer (containing rAAV) is diluted 5-fold with a no-salt dilution buffer (pH varying depending on serotype) and applied to a Hi-Trap HP-Q/S column. Upon elution with a NaCl salt gradient, peak 1 ml fractions (typically 3-5) are pooled, dialyzed with T20M1N200 (pH 8.0), then sterile filtered and supplemented with 0.001% Pluronic F68. Vectors are stored at −80° C. Purified virus was titered for vg using Q-PCR as previously described (Schnepp and Clark, Methods Mol. Med., 69:427-443 (2002)).